Multiplicação in vitro de gemas axilares de acácia-negra (Acacia mearnsii De Wild.) In vitro multiplication of black watlle (Acacia mearnsii De Wild.) axillary buds

A acácia-negra (Acacia mearnsii De Wild.) é uma espécie de difícil micropropagação devido à pequena capacidade de multiplicação e desenvolvimento de gemas. O presente estudo visou determinar a influência de diferentes citocininas na proliferação de gemas axilares em segmentos nodais de A. mearnsii. Plântulas germinadas in vitro forneceram explantes que foram inoculadas em meio básico B5 (GAMBORG et al., 1968). Testaram-se as citocininas: BAP (6-benzilaminopurina), BA (6-benziladenosina), 2iP (gama,gama-isopenteniladenina) e cinetina (6-furfuralamino-purina). Diferentes concentrações desses reguladores de crescimento foram empregadas: 1 mgL-1, 2 mgL-1 e 3 mgL-1. Utilizou-se o delineamento de blocos casualizados, em arranjo fatorial, com seis repetições e cinco plantas por parcela. As avaliações foram feitas aos 30 dias, através da contagem de gemas alongadas e da presença de calos. A utilização de BAP a 2 mgL-1 promoveu a maior taxa de multiplicação de gemas (3,5 brotos/explante).<br>Black wattle (Acacia mearnsii de Wild.) is difficult to micropropagate due to the low ability of multiplication and development of shoots. Thus, the present study aimed at determining the influence of various cytokinins on axillary bud proliferation in nodal segments of A. mearnsii. Explants from in vitro germinated seedlings were inoculated on B5 (Gamborg et al., 1968) basal medium. BAP (6-benzylaminopurine), BA (6-benzyladenine), 2iP (gamma,gamma-dimethylallylamino-purine) and Kinetin (6-furfurylaminopurine) were tested at the concentrations 1 mgL-1, 2 mgL-1, and 3 mgL-1. A randomized block design, in factorial arrangement with 6 replications, and 5 plants per plot was used. The assessments were made after 30 days, by counting the elongated shoots and the presence of callus. The use of BAP at 2 mgL-1 promoted the highest rate of bud multiplication (3,5 shoots/explant)

Do disease specific characteristics add to the explanation of mobility limitations in patients with different chronic diseases? A study in The Netherlands.

STUDY OBJECTIVES: To determine whether disease specific characteristics, reflecting clinical disease severity, add to the explanation of mobility limitations in patients with specific chronic diseases. DESIGN AND SETTING: Cross sectional study of survey data from community dwelling elderly people, aged 55-85 years, in the Netherlands. PARTICIPANTS AND METHODS: The additional explanation of mobility limitations by disease specific characteristics was examined by logistic regression analyses on data from 2830 community dwelling elderly people. MAIN RESULTS: In the total sample, chronic non-specific lung disease, cardiac disease, peripheral atherosclerosis, diabetes mellitus, stroke, arthritis and cancer (the index diseases), were all independently associated with mobility limitations. Adjusted for age, sex, comorbidity, and medical treatment disease specific characteristics that explain the association between disease and mobility mostly reflect decreased endurance capacity (shortness of breath and disturbed night rest in chronic non-specific lung disease, angina pectoris and congestive heart failure in cardiac disease), or are directly related to mobility function (stiffness and lower body complaints in arthritis). For atherosclerosis and diabetes mellitus, disease specific characteristics did not add to the explanation of mobility limitations. CONCLUSIONS: The results provide evidence that, to obtain more detailed information about the differential impact of chronic diseases on mobility, disease specific characteristics are important to take into account

Family characteristics of Indian parasuicide patients : a controlled study

In this article a controlled study of the family structure of a South African Indian parasuicide population is described. Twenty subjects from a local general hospital were scored on the Suicidal Intent Scale (SIS) and the Family Assessment Device (FAD). Twenty matched medical patients without a history of parasuicide were selected from the same hospital as a control group. They were matched for age, sex, educational standard, ethnic group and socio-economic status. All subjects were scored on the Family Assessment Device (FAD). Subjects from both groups were re-tested between six to eight weeks after the initial assessment. Analyses of variance indicated significant differences between the two groups on indicators of family interactional pathology. This seems to be compounded by family stresses emanating from socio-cultural transition. The results of the study support the view that family therapy should be implemented in the treatment of parasuicide. The unique population under study provides cross-cultural data relevant to parasuicide research and invites possibilities for further investigationPeer reviewe