Avaliação de tetraplóides de bananeira cultivados em área infestada por mal-do-Panamá: segundo ciclo.

O mal-do-Panamá uma das mais importantes doenças da bananeira, causada pelo fungo Fusarium oxysporum f.sp. cubense, provoca elevadas perdas na produção. Esse trabalho teve como objetivo, avaliar, no segundo ciclo, as características agronômicas e a reação ao Fusarium de genótipos de bananeira. Foram avaliadas nove cultivares usando a Maçã como testemunha (suscetível), em delineamento inteiramente casualizado, com 10 repetições de uma planta. Avaliaram-se oito características agronômicas e a incidência de Fusarium. Há uma ampla variação genética para todos os caracteres agronômicos avaliados. Os híbridos de Prata Anã são superiores em produção aos de Pacovan. Os genótipos Porp, P.Jussara, Fhia-01 e PA94-01 são resistentes ao mal-do-Panamá.pdf 218

Evaluation of Musa spp. for resistance to moko disease (Ralstonia solanacearum, race 2).

The objetive this paper was to evaluate the reaction of 31 diploid (AA) genotypes to the inoculation with R. solanacearum, race 2, aiming at selecting resistant ones to be used as male parents in the banana breeding programme under conduction at the Embrapa Cassava and Fruit Crops (EMBRAPA-CNPMF), in the Manaus-AM (Brazil)

Computational Modelling and Sustainable Synthesis of a Highly Selective Electrochemical MIP-Based Sensor for Citalopram Detection

A novel molecularly imprinted polymer (MIP) has been developed based on a simple and sustainable strategy for the selective determination of citalopram (CTL) using screen-printed carbon electrodes (SPCEs). The MIP layer was prepared by electrochemical in situ polymerization of the 3-amino-4 hydroxybenzoic acid (AHBA) functional monomer and CTL as a template molecule. To simulate the polymerization mixture and predict the most suitable ratio between the template and functional monomer, computational studies, namely molecular dynamics (MD) simulations, were carried out. During the experimental preparation process, essential parameters controlling the performance of the MIP sensor, including CTL:AHBA concentration, number of polymerization cycles, and square wave voltammetry (SWV) frequency were investigated and optimized. The electrochemical characteristics of the prepared MIP sensor were evaluated by both cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) techniques. Based on the optimal conditions, a linear electrochemical response of the sensor was obtained by SWV measurements from 0.1 to 1.25 µmol L−1 with a limit of detection (LOD) of 0.162 µmol L−1 (S/N = 3). Moreover, the MIP sensor revealed excellent CTL selectivity against very close analogues, as well as high imprinting factor of 22. Its applicability in spiked river water samples demonstrated its potential for adequate monitoring of CTL. This sensor offers a facile strategy to achieve portability while expressing a willingness to care for the environmentPatrícia Rebelo (SFRH/BD/132384/2017) and João Pacheco (SFRH/BPD/101419/2014) are grateful to Fundação para a Ciência e a Tecnologia (FCT) and European Union (EU) for their grants, financed by POPH-QREN-Tipologia 4.1-Formação Avançada, funded by Fundo Social Europeu (FSE) and Ministério da Ciência, Tecnologia e Ensino Superior (MCTES). Isabel Seguro is grateful to the project Farmasense (39957) that was funded by Sistema de Incentivos à Investigação e Desenvolvimento Tecnológico de Portugal 2020, through the Programa Operacional do Norte (NORTE 2020) and the Fundo Europeu de Desenvolvimento Regional (FEDER). This work was further supported by UID/QUI/50006/2020 (LAQV-REQUIMTE) and the project PTDC/QUI-QAN/3899/2021 with funding from FCT/MCTES through national funds. The research was funded also by FCT and BiodivRestore Joint Call 2020–2021-European Union’s Horizon 2020 research and innovation programme under grant agreement No 101003777-BiodivRestore-406/DivRestore/0002/2020-BioReset-“Biodiversity restoration and conservation of inland water ecosystems for environmental and human well-being”info:eu-repo/semantics/publishedVersio

Integrated polarizers based on tapered highly birefringent photonic crystal fibers

COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPFINANCIADORA DE ESTUDOS E PROJETOS - FINEPThis paper proposes and demonstrates the creation of sections with a high polarization dependent loss (PDL) in a commercial highly birefringent (polarization maintaining) photonic crystal fiber (PCF), via tapering with pressure applied to the holes. The tapers had a 1-cm-long uniform section with a 66% scale reduction, in which the original microstructure aspect ratio was kept by the pressure application. The resulting waveguides show polarizing action across the entire tested wavelength range, 1510-1600 nm, with a peak PDL of 35.3 dB/cm (c.f. ∼1 dB/cm for a typical commercial polarizing fiber). The resulting structure, as well as its production, is extremely simple, and enable a small section with a high PDL to be obtained in a polarization maintaining PCF, meaning that the polarization axes in the polarizing and polarization maintaining sections are automatically aligned. © 2014 Optical Society of America.This paper proposes and demonstrates the creation of sections with a high polarization dependent loss (PDL) in a commercial highly birefringent (polarization maintaining) photonic crystal fiber (PCF), via tapering with pressure applied to the holes. The tapers had a 1-cm-long uniform section with a 66% scale reduction, in which the original microstructure aspect ratio was kept by the pressure application. The resulting waveguides show polarizing action across the entire tested wavelength range, 1510-1600 nm, with a peak PDL of 35.3 dB/cm (c.f. similar to 1 dB/cm for a typical commercial polarizing fiber). The resulting structure, as well as its production, is extremely simple, and enable a small section with a high PDL to be obtained in a polarization maintaining PCF, meaning that the polarization axes in the polarizing and polarization maintaining sections are automatically aligned.22151776917775COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPFINANCIADORA DE ESTUDOS E PROJETOS - FINEPCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPFINANCIADORA DE ESTUDOS E PROJETOS - FINEPSem informaçãoSem informaçãoSem informação0.1.06.1177.00Chraplyvy, A.R., High-capacity lightwave transmission experiments (1999) Bell Labs Tech. J., 4 (1), pp. 230-245Bock, W.J., Chen, J., Eftimov, T., Urbanczyk, W., A photonic crystal fiber sensor for pressure measurements (2006) IEEE Trans. Instrum. Meas., 55 (4), pp. 1119-1123Bergh, R.A., Lefevre, H.C., Shaw, H.J., An overview of fiber-optic gyroscopes (1984) J. Lightwave Technol., 2 (2), pp. 91-107Jacobsen, W., Mayfield, J., Fournier, P., Bolte, D., Elmaola, H., Wang, C.H., Drenzek, G., Soufiane, A., (2013) Single-polarization Fiber, , Verrillon Inc., US Patent 8, 369, 672 B2Nolan, D.A., Berkey, G.E., Li, M.J., Chen, X., Wood, W.A., Zenteno, L.A., Single-polarization fiber with a high extinction ratio (2004) Opt. Lett., 29 (16), pp. 1855-1857Russell, P., Photonic crystal fibers (2006) J. Lightwave Technol., 24 (12), pp. 4729-4749Saitoh, K., Koshiba, M., Single-polarization single-mode photonic crystal fibers (2003) IEEE Photon. Technol. Lett., 15 (10), pp. 1384-1386Ju, J., Jin, W., Demokan, M.S., Design of single-polarization single-mode photonic crystal fiber at 1.30 and 1.55 μm (2006) J. Lightwave Technol., 24 (2), pp. 825-830Zhang, F., Zhang, M., Liu, X., Ye, P., Design of wideband single-polarization single-mode photonic crystal fiber (2007) J. Lightwave Technol., 25 (5), pp. 1184-1189Chen, M.Y., Sun, B., Zhang, Y.K., Broadband single-polarization operation in square-lattice photonic crystal fibers (2010) J. Lightwave Technol., 28 (10), pp. 1443-1446Serrão, V.A., Franco, M.A.R., A new approach to obtain single-polarization hollow-core photonic bandgap fiber (2013) Proc. SPIE 8794, Fifth European Workshop on Optical Fibre Sensors, 879428Kubota, H., Kawanishi, S., Koyanagi, S., Tanaka, M., Yamaguchi, S., Absolutely single polarization photonic crystal fiber (2004) IEEE Photon. Technol. Lett., 16 (1), pp. 182-184Statkiewicz-Barabach, G., Olszewski, J., Napiorkowski, M., Golojuch, G., Martynkien, T., Tarnowski, K., Urbanczyk, W., Thienpont, H., Polarizing photonic crystal fiber with lowindex inclusion in the core (2010) J. Opt., 12 (7), p. 075402Zheng, X., Liu, Y., Wang, Z., Han, T., Tai, B., Tunable single-polarization single mode photonic crystal fiber based on liquid infiltrating (2011) IEEE Photon. Technol. Lett., 23, pp. 709-711Espinel, A.V.Y., Franco, M.A.R., Cordeiro, C.M.B., Tunable single-polarization single-mode microstructure polymer optical fiber (2011) J. Lightwave Technol., 29 (16), pp. 2372-2378Qian, W., Zhao, C.L., Wang, Y., Chan, C.C., Liu, S., Jin, W., Partially liquid-filled hollow-core photonic crystal fiber polarizer (2011) Opt. Lett., 36 (16), pp. 3296-3298Sodré Jr., A.C., Nascimento Jr., A.R., Franco, M.A.R., Oliveira, I., Serrão, V.A., Fragnito, H.L., Numerical and experimental analysis of polarization properties from hybrid PCFs across different photonic bandgaps (2012) Opt. Fiber Technol., 18 (6), pp. 462-469Romagnoli, P., Biazoli, C.R., Franco, M.A.R., Cordeiro, C.M.B., De Matos, C.J.S., Generation of polarizing sections in highly birefringent photonic crystal fibers via post-processing (2013) CLEO:2013, , Optical Society of America, paper JTu4A.12Birks, T.A., Li, Y.W., The shape of fiber tapers (1992) J. Lightwave Technol., 10 (4), pp. 432-438Ju, J., Jin, W., Yang, Y., Introduction of birefringence into photonic crystal fibers (2011) Proc. SPIE 7753, 21st International Conference on Optical Fiber Sensors, 77536JMalitson, I.H., Interspecimen comparison of the refractive index of fused silica (1965) J. Opt. Soc. Am., 55 (10), pp. 1205-1209Gerosa, R.M., Spadoti, D.H., Menezes, L.S., De Matos, C.J.S., In-fiber modal Mach-Zehnder interferometer based on the locally post-processed core of a photonic crystal fiber (2011) Opt. Express, 19 (4), pp. 3124-3129The authors thank CAPES, Mackpesquisa, INCT Fotonicom (CNPq and FAPESP) and FINEP (SIA Project- proc. 0.1.06.1177.00) for financial support

Avaliação de genótipos de bananeira quanto à resistência ao mal-do-Panamá em condições de campo.

A banana (Musa spp.) é a fruta mais consumida no mundo. Dentre as doenças que ocorre na cultura, o mal-do-Panamá, causado por Fusarium oxysporum f. sp. cubense ? Foc, é altamente destrutivo. A medida de controle mais efetiva é o uso de variedades resistentes. Contudo, a maioria das cultivares plantadas no Brasil são suscetíveis ao patógeno, fato que conduz a severas perdas no rendimento da cultura

Avaliação de genótipos de bananeira quanto a resistência ao mal-do-Panamá em condições de campo.

Considerando que a grande maioria das variedades cultivadas no Brasil é suscetível ao mal-do-Panamá e as perdas no rendimento podem alcançar 100%, o uso de variedades resistentes é a única medida eficiente de controle dessa doença. O trabalho teve como objetivo avaliar genótipos oriundos do programa de melhoramento genético da bananeira da Embrapa Mandioca e Fruticultura quanto à resistência a mal-do-Panamá em condições de campo artificialmente infestado com Fusarium oxysporum f. sp. Cubense

Soil organic matter fractions as a short-term indicator of carbon turnover under agricultural systems.

Integrated crop-livestock-forest (ICLF) systems has been considered as an important strategy to enhance C sequestration. However, changes in soil C due to agricultural management practices, in general, are observed in long-term experiments. Our objective was to investigate short-term impacts on soil organic matter under different management systems in the Cerrado/Amazonia region. Treatments corresponded to ICLF, No-tillage, Pasture and Eucalyptus plantation. Soil was sampled in 2014, in the 0-5, 5-10 and 10-30 cm layers for quantification of light- and heavy-fraction C stocks as well as their C isotopic composition (DELTA 13C). After three years established, all evaluated treatments showed similar values of light- and heavy-fraction C stocks to those observed under Native Forest in the 0-30 cm layer. Under Pasture and ILCF treatments, we observed that most of C of the light-fraction was substituted by pasturederived C in the uppermost soil layer (0-5 cm). Despite no differences in heavyand light-fraction C, the application of 13C stable isotope technique seems to be an important tool to evaluate short-term C turnover under agricultural systems

Soil organic matter fractions as a short-term indicator of carbon turnover under agricultural systems.

Integrated crop-livestock-forest (ICLF) systems has been considered as an important strategy to enhance C sequestration. However, changes in soil C due to agricultural management practices, in general, are observed in long-term experiments. Our objective was to investigate short-term impacts on soil organic matter under different management systems in the Cerrado/Amazonia region. Treatments corresponded to ICLF, No-tillage, Pasture and Eucalyptus plantation. Soil was sampled in 2014, in the 0-5, 5-10 and 10-30 cm layers for quantification of light- and heavy-fraction C stocks as well as their C isotopic composition (DELTA 13C). After three years established, all evaluated treatments showed similar values of light- and heavy-fraction C stocks to those observed under Native Forest in the 0-30 cm layer. Under Pasture and ILCF treatments, we observed that most of C of the light-fraction was substituted by pasturederived C in the uppermost soil layer (0-5 cm). Despite no differences in heavyand light-fraction C, the application of 13C stable isotope technique seems to be an important tool to evaluate short-term C turnover under agricultural systems

X-linked Adrenal Hypoplasia Congenita: report of two families and a new NR0B1 mutation

Background: X-linked Adrenal Hypoplasia Congenita (X-linked AHC) tipically manifests as adrenal insufficiency in a bimodal clinical presentation (5–60 days and 2–13 years) and, at pubertal age, hypogonadotropic hypogonadism (HH) in males. It is caused by mutations in NR0B1 (present in Xp21.2), a gene with a critical role in the development of adrenals and hypothalamic-pituitary-gonadal (HPG) axis. It represents ~1% of all causes of primary adrenal insufficiency under age 18. We present two kindreds with NR0B1 mutations, one with a previously unreported mutation. Clinical case - family A: The proband presented with adrenal crisis at day 18 of life. Family history was relevant for an uncle death as a newborn. Hormonal assays revealed elevated ACTH (34,5 pmol/L, reference: T, leading to a premature stop codon, p.Lys362*, in exon 1. His mother and sister were both asymptomatic carriers for this mutation. This mutation, not described previously, leads probably to a non-functional truncated protein. Clinical case - family B: Kindred B had two males who presented with adrenal crisis at the newborn age (proband: 14 days; brother: 16 days). At 6 months of age, the proband had elevated ACTH (193.2 pmol/L, reference: G; p.Tyr81*, in exon 1, present in the two affected males. His mother and one sister were also asymptomatic carriers. Conclusion: Our kindreds highlights the clinical aspects of the rare X-linked AHC and its challenging differential diagnosis. The diagnosis was based on …. Completar … and an apparently X-linked mode of inheritance. Molecular analysis of the NR0B1 gene allowed the identification of the molecular defect in both families. Two different nonsense mutation in exon 1 were identified, giving rise most probably to truncated non-functional proteins, confirming the disease severity or the clinical phenotype of the affected boys. Mutation identification was relevant for genetic counseling of the parents, as well as for the patient’s sisters