HIV-positiivsete inimeste antiretroviirusravi soostumus ja seda mõjutavad tegurid

http://www.ester.ee/record=b4683587*es

Revue industrielle : revue mensuelle technique et économique

19071907 (A38,N1)- (A38,N52)

Additional file 2: Table S1. of Transcriptome-wide analysis supports environmental adaptations of two Pinus pinaster populations from contrasting habitats

Limma DE gene table of the spots with foreground different to background. Table S2. EdgeR DE gene table. Table S3. Comparison between PINARRA3 and RNA-Seq data. Table S4. Mapman categories from DE genes. Table S5. Contig ID for Reference transcriptome. Table S6. Primers used for qPCR. (XLSX 4413 kb

Virtual prototype of mechatronic system

This thesis deals with modeling of delta 3D printer virtual prototype and its co-simulation using software ADAMS and MATLAB/SIMULINK. The first part consists of virtual model creation and its kinematic analysis. Then a model of electric motors is created. The final part consists of co-simulation and control design

Additional file 8: Figure S4. of Establishing gene models from the Pinus pinaster genome using gene capture and BAC sequencing

Alignment of the PAT gene promoter [GenBank:HE866755], to the gene capture PAT gene 5´upstream region. (TIF 332 kb

Cellulose and lignin content in <i>PpDof5</i> transgenic poplar.

<p>(A) Determination of cellulose and lignin content in 10-week-old poplar plants grown in a growth-chamber. Values are expressed as mg/g FW. The determination was performed in triplicate in three independent biological samples. Asterisks indicate significant differences between controls and lines by the <i>t</i>-test (P<0.05). (B) Determination of cellulose and lignin content in poplar trees grown in the field. Values are expressed as mg/g FW. The determination is the mean of three independent biological samples measured in triplicate.</p

Field trial of transgenic poplar overexpressing <i>PpDof5</i>.

<p>(A) Diagram of the ground for the field trial experiment. (B) General overview of ground-field trees in the field trial. (C) Confirmation of the presence of the transgene in field-growing trees. PCR analysis was performed using primers specific for <i>PpDof5</i> on genomic DNA from poplar. As a control, the internal poplar gene <i>PtGS2</i> was amplified. Two kinds of samples from years 2012 and 2013, the two growing periods considered, were analyzed. (D) The mRNA level of <i>PpDof5</i> was determined by qPCR in leaves of field-growing trees in 2012 and 2013. Determinations were made in triplicate. (E) Content of chlorophyll a and b in controls and transgenic poplar was determined in the upper (UL) and basal (BL) leaves of trees during the growing period of 2012 and in leaves and branches during the growing period of 2013. White bars correspond to control samples, gray bars are L2 and black bars are L6 samples.</p

Expression analysis of <i>PAL1</i> and <i>GS1</i>.<i>3</i>.

<p>(A) Diagram of the location in the pathway of the two enzymes involved in the synthesis of phenylalanine for lignin synthesis. The ammonium flux involving the two enzymatic reactions is highlighted in black. (B) Expression analysis by qPCR of <i>PAL1</i> and <i>GS1</i>.<i>3</i> genes in the basal section of the stem in control and transgenic poplar trees. Measurements were performed in triplicate in three independent biological replicas. Asterisks indicate that the difference between the control and transgenic poplars is significant by the <i>t</i>-test (P<0.05).</p

Analysis of the transgenic poplar lines.

<p>(A) Diagram of the construct used to transform hybrid poplar clone INRA 7171-B <i>(P</i>. <i>tremula x P</i>. <i>alba)</i>: the pBI121 vector containing the <i>PpDof5</i> sequence under the control of a 35S gene promoter (CaMV35S) was used for the transformation. (B) Determination of copy number in the L2 and L6 transgenic lines using quantitative PCR. The poplar single copy gene FAD-dependent oxidoreductase (Potri.018G112600) was used as the standard. The ratio of the amplicons derived from the integrated <i>PpDof5</i> and the internal control is indicated at the bottom of each line. (C) Expression analysis of <i>PpDof5</i> by real-time qPCR in controls (white bar) and two transgenic lines: L2 (gray bar) and L6 (black bar). The expression data were normalized using ubiquitin as the reference gene. (D) Representative picture of 10- week-old untransformed control and L2 and L6 transgenics. (E) Root volume of control and transgenic poplar plants. (F) Length of the stem in cm of the plants (five plants were used for each determination).</p

¹⁵N uptake in hydroponic culture.

<p>(A) Plants acclimatized in transparent plastic pots as described in Materials and Methods and grown in KNO₃ as the sole N source were incubated for 2 h in the same solution but containing ¹⁵N-label as 1 mM K¹⁵NO₃. δ¹⁵N (‰) values were determined in roots using an elemental analyzer coupled to a Delta V Advantage isotope ratio mass spectrometer. Values are in triplicate, and asterisks indicate that the difference between the control and transgenic poplars is significant by the <i>t</i>-test (P<0.05).(B) The same as in (A), except that plants were grown under NH₄Cl as the sole N source.</p