2,796 research outputs found

    Regulation of cAMP responses by the G12/13 pathway converges on adenylyl cyclase VII

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    Regulation of intracellular cyclic adenosine 3’, 5’-monophosphate (cAMP) by multiple pathways enables differential function of this ubiquitous second messenger in a context dependent manner. Modulation of Gs-stimulated intracellular cAMP has long been known to be modulated by the Gi and Gq/Ca2+ pathways. Recently, the G13 pathway was also shown to facilitate cAMP responses in murine macrophage cells. We report here that this synergistic regulation of cAMP synthesis by the Gs and G13 pathways is mediated by a specific isoform of adenylyl cyclase, AC7. Furthermore, this signaling paradigm exists in several hematopoietic lineages and can be recapitulated by exogenous expression of AC7 in HEK 293 cells. Mechanistic characterization of this synergistic interaction indicates that it occurs downstream of receptor activation and it can be mediated by the alpha subunit of either G12 or G13. Our results demonstrate that AC7 is a specific downstream effector of the G12/13 pathway

    Scaled Physical Prototyping of Construction Processes Using 3D Printing

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    3D printing and Building Information Modelling (BIM) are two technologies that have become increasingly popular and changed a wide variety of applications in a significant manner. 3D printing enables the design, prototyping and manufacture of items that were previously impossible or infeasible to do. Similarly, BIM is changing the way building and civil infrastructure projects are conceived, designed, delivered and maintained with a push towards a digital, wholly collaborative construction environment.This paper examines how 3D printing and BIM can be used in a complimentary manner to help visualise and communicate important aspects of a project to the relevant stakeholders and personnel involved. By using the BIM model that has been developed for a specific project and segmenting key components of this model we can then use 3D printers to create a scaled model of the job and educate the concerned parties in the construction methodology, main risk areas, and ongoing concerns for maintenance and end-of-life deconstruction.

    Impact of the Algebra I End of Course Examination on African American Students Obtaining a Standard High School Diploma

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    The state of Florida requires all students complete Algebra I and pass the End of Course Examination (EOCE) to graduate with a standard high school diploma. Algebra I EOCE results indicate that many African American students do not pass the examination. This research sought to determine if there is a relationship between African American students’ failure to pass the Algebra I EOCE and graduate with a standard diploma. Four hypotheses, null and alternative were tested. Two ninth-grade cohorts, 2013-2014 and 2014-2015 comprised the sample. Data were analyzed using t test and one-way analyses of variance (ANOVA). Results indicate a significant relationship between African American students’ failure to pass the Algebra I EOCE and graduation with a standard high school diploma

    Hard diffraction from parton rescattering in QCD

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    We analyze the QCD dynamics of diffractive deep inelastic scattering. The presence of a rapidity gap between the target and diffractive system requires that the target remnant emerges in a color singlet state, which we show is made possible by the soft rescattering of the struck quark. This rescattering is described by the path-ordered exponential (Wilson line) in the expression for the parton distribution function of the target. The multiple scattering of the struck parton via instantaneous interactions in the target generates dominantly imaginary diffractive amplitudes, giving rise to an "effective pomeron" exchange. The pomeron is not an intrinsic part of the proton but a dynamical effect of the interaction. This picture also applies to diffraction in hadron-initiated processes. Due to the different color environment the rescattering is different in virtual photon- and hadron-induced processes, explaining the observed non-universality of diffractive parton distributions. This framework provides a theoretical basis for the phenomenologically successful Soft Color Interaction model which includes rescattering effects and thus generates a variety of final states with rapidity gaps. We discuss developments of the SCI model to account for the color coherence features of the underlying subprocesses.Comment: 12 pages, 9 figures, REVTeX4. Somewhat expanded and modified version, two new subsections added. To appear in PR

    Quantum Key Distribution in a Multi-User Network at Gigahertz Clock rates

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    In recent years quantum information research has lead to the discovery of a number of remarkable new paradigms for information processing and communication. These developments include quantum cryptography schemes that offer unconditionally secure information transport guaranteed by quantum-mechanical laws. Such potentially disruptive security technologies could be of high strategic and economic value in the future. Two major issues confronting researchers in this field are the transmission range (typically <100km) and the key exchange rate, which can be as low as a few bits per second at long optical fiber distances. This paper describes further research of an approach to significantly enhance the key exchange rate in an optical fiber system at distances in the range of 1-20km. We will present results on a number of application scenarios, including point-to-point links and multi-user networks. Quantum key distribution systems have been developed, which use standard telecommunications optical fiber, and which are capable of operating at clock rates of up to 2GHz. They implement a polarization-encoded version of the B92 protocol and employ vertical-cavity surface-emitting lasers with emission wavelengths of 850 nm as weak coherent light sources, as well as silicon single-photon avalanche diodes as the single photon detectors. The point-to-point quantum key distribution system exhibited a quantum bit error rate of 1.4%, and an estimated net bit rate greater than 100,000 bits- per second for a 4.2 km transmission range.Comment: Presented at SPIE Symposium on Microtechnologies for the New Millennium, Sevilla, Spain (May 2005
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