Growth and population dynamics of crayfish Paranephrops planifrons in streams within native forest and pastoral land uses

Population dynamics of crayfish (Paranephrops planifrons White) in streams draining native forest and pastoral catchments, Waikato, New Zealand, were investigated from September 1996 to July 1998. Crayfish densities were generally greater in native forest streams because of high recruitment over summer, but varied greatly between streams in both land uses. Peak densities in summer were 9 crayfish m-2 in native forest and 6 crayfish m-2 in pasture streams, but peak biomass in summer was much greater in pasture streams. Mark-recapture data showed that crayfish, particularly juveniles, in pasture streams grew faster than in native forest streams, through both greater moult frequency and larger moult increments. Females reached reproductive size at c. 20 mm orbit-carapace length (OCL) after their first year in pasture streams, but after 2 years in native forest streams. Annual degree days >10°C appeared to explain the differences in the timing of life cycles. Estimates of annual crayfish production (range = 0.8-3.4 g dry weight m-2 year-1) were similar in both land uses, and P/B ratios were between 0.95 and 1.2. Despite deforestation and conversion to pasture, crayfish in these Waikato hill-country streams have maintained similar levels of annual production to those in native forest streams, although juvenile growth rates have increased and longevity has decreased

Evaluating techniques for sampling stream crayfish (paranephrops planifrons)

We evaluated several capture and analysis techniques for estimating abundance and size structure of freshwater crayfish (Paranephrops planifrons) (koura) from a forested North Island, New Zealand stream to provide a methodological basis for future population studies. Direct observation at night and collecting with baited traps were not considered useful. A quadrat sampler was highly biased toward collecting small individuals. Handnetting at night and estimating abundances using the depletion method were not as efficient as handnetting on different dates and analysing by a mark-recapture technique. Electrofishing was effective in collecting koura from different habitats and resulted in the highest abundance estimates, and mark-recapture estimates appeared to be more precise than depletion estimates, especially if multiple recaptures were made. Handnetting captured more large crayfish relative to electrofishing or the quadrat sampler

Wild record of an apple snail in the Waikato River, Hamilton, New Zealand and their incidence in freshwater aquaria

We report the discovery of a single specimen of a live apple snail Pomacea diffusa Blume 1957 (Ampullariidae: Prosobranchia), from the Waikato River, Hamilton city, central North Island, New Zealand. This species, along with the congeneric P. insularum, is imported for the aquarium trade, and its occurrence in the river likely stemmed from an aquarium release. A survey of 55 aquaria belonging to 43 hobbyists revealed 27 apple snails, with one owner having 22 snails. Assessment of environmental tolerances and impacts of P. diffusa, based largely on studies of the closely related and commonly confused congener P. bridgesii, suggests that direct habitat impacts by this species are likely to be minor. However, there could be indirect influences on native biodiversity through predation on eggs or competition for food supplies with other detritivorous species if densities were to become high. Water temperatures in the Waikato River below Hamilton (10-23˚C in 2009) may enable released individuals to persist for an extended period, and over summer may exceed the threshold required to enable breeding. However, population establishment would be most likely in locations where water is heated through geothermal influences or industrial cooling water discharges

UV Spectroscopy of AB Doradus with the Hubble Space Telescope. Impulsive flares and bimodal profiles of the CIV 1549 line in a young star

We observed AB Doradus, a young and active late type star (K0 - K2 IV-V, P= 0.514 d) with the Goddard High Resolution Spectrograph of the post-COSTAR Hubble Space Telescope with the time and spectral resolutions of 27 s and 15 km, respectively. The wavelength band (1531 - 1565 A) included the strong CIV doublet (1548.202 and 1550.774, formed in the transition region at 100 000 K). The mean quiescent CIV flux state was close to the saturated value and 100 times the solar one. The line profile (after removing the rotational and instrumental profiles) is bimodal consisting of two Gaussians, narrow (FWHM = 70 km/s) and broad (FWHM =330km/s). This bimodality is probably due to two separate broadening mechanisms and velocity fields at the coronal base. It is possible that TR transient events (random multiple velocities), with a large surface coverage, give rise to the broadening of the narrow component,while true microflaring is responsible for the broad one. The transition region was observed to flare frequently on different time scales and magnitudes. The largest impulsive flare seen in the CIV 1549 emission reached in less than one minute the peak differential emission measure (10**51.2 cm-3) and returned exponentially in 5 minutes to the 7 times lower quiescent level.The 3 min average line profile of the flare was blue-shifted (-190 km/s) and broadened (FWHM = 800 km/s). This impulsive flare could have been due to a chromospheric heating and subsequent evaporation by an electron beam, accelerated (by reconnection) at the apex of a coronal loop.Comment: to be published in AJ (April 98), 3 tables and 7 figures as separate PS-files, print Table 2 as a landscap

Williopsis saturnus yeast killer toxin does not kill Streptococcus pneumoniae

Streptococcus pneumoniae is an important human bacterial pathogen, and the increase in antibiotic resistance demands the development of new antimicrobial compounds. Several reports have suggested that yeast killer toxins show activity against bacteria and we therefore investigated the activity of K9 killer toxin from the yeast Williopsis saturnus var. mrakii NCYC 500 against S. pneumoniae. However, no inhibition of bacterial growth was observed with concentrated K9 preparations in agar diffusion assays and in liquid culture. Although cell morphology was slightly affected by K9 treatment, no effect on cellular viability was detectable, and K9 had no stimulatory effect on cell lysis induced by β-lactams or Triton X-100. This indicated that K9 did not contribute to cell wall damage. Moreover, flow cytometry was used as a sensitive assessment of integrity of cells exposed to killer toxin. No significant damage of S. pneumoniae cells was evident, although minor changes in fluorescence suggested that K9 killer toxin may interact with bacterial surface components

Pattern formation by lateral inhibition with feedback: a mathematical model of Delta-Notch intercellular signalling

In many developing tissues, adjacent cells diverge in character so as to create a fine-grained pattern of cells in contrasting states of differentiation. It has been proposed that such patterns can be generated through lateral inhibition—a type cells–cell interaction whereby a cell that adopts a particular fate inhibits its immediate neighbours from doing likewise. Lateral inhibition is well documented in flies, worms and vertebrates. In all of these organisms, the transmembrane proteins Notch and Delta (or their homologues) have been identified as mediators of the interaction—Notch as receptor, Delta as its ligand on adjacent cells. However, it is not clear under precisely what conditions the Delta-Notch mechanism of lateral inhibition can generate the observed types of pattern, or indeed whether this mechanism is capable of generating such patterns by itself. Here we construct and analyse a simple and general mathematical model of such contact-mediated lateral inhibition. In accordance with experimental data, the model postulates that receipt of inhibition (i.e. activation of Notch) diminishes the ability to deliver inhibition (i.e. to produce active Delta). This gives rise to a feedback loop that can amplify differences between adjacent cells. We investigate the pattern-forming potential and temporal behavior of this model both analytically and through numerical simulation. Inhomogeneities are self-amplifying and develop without need of any other machinery, provided the feedback is sufficiently strong. For a wide range of initial and boundary conditions, the model generates fine-grained patterns similar to those observed in living systems

Rotationally Modulated X-ray Emission from T Tauri Stars

We have modelled the rotational modulation of X-ray emission from T Tauri stars assuming that they have isothermal, magnetically confined coronae. By extrapolating surface magnetograms we find that T Tauri coronae are compact and clumpy, such that rotational modulation arises from X-ray emitting regions being eclipsed as the star rotates. Emitting regions are close to the stellar surface and inhomogeneously distributed about the star. However some regions of the stellar surface, which contain wind bearing open field lines, are dark in X-rays. From simulated X-ray light curves, obtained using stellar parameters from the Chandra Orion Ultradeep Project, we calculate X-ray periods and make comparisons with optically determined rotation periods. We find that X-ray periods are typically equal to, or are half of, the optical periods. Further, we find that X-ray periods are dependent upon the stellar inclination, but that the ratio of X-ray to optical period is independent of stellar mass and radius.Comment: 10 pages, 8 figures, accepted for publication in MNRA

Bistable molecular conductors with a field-switchable dipole group

A class of bistable "stator-rotor" molecules is proposed, where a stationary bridge (stator) connects the two electrodes and facilitates electron transport between them. The rotor part, which has a large dipole moment, is attached to an atom of the stator via a single sigma bond. Hydrogen bonds formed between the rotor and stator make the symmetric orientation of the dipole unstable. The rotor has two potential minima with equal energy for rotation about the sigma bond. The dipole orientation, which determines the conduction state of the molecule, can be switched by an external electric field that changes the relative energy of the two potential minima. Both orientation of the rotor correspond to asymmetric current-voltage characteristics that are the reverse of each other, so they are distinguishable electrically. Such bistable stator-rotor molecules could potentially be used as parts of molecular electronic devices.Comment: 8 pages, 7 figure