Continuation of research in software for space operations support

Software technologies relevant to workstation executives are discussed. Evaluations of problems, potential or otherwise, seen with IBM's Workstation Executive (WEX) 2.5 preliminary design and applicable portions of the 2.5 critical design are presented. Diverse graphics requirements of the Johnson Space Center's Mission Control Center Upgrade (MCCU) are also discussed. The key is to use tools that are portable, compatible with the X window system, and best suited to the requirements of the associated application. This will include a User Interface Language (UIL), an interactive display builder, and a graphic plotting/modeling system. Work sheets are provided for POSIX 1003.4 real-time extensions and the requirements for the Center's automated information systems security plan, referred to as POSIX 1003.6, are discussed

Research into software executives for space operations support

Research concepts pertaining to a software (workstation) executive which will support a distributed processing command and control system characterized by high-performance graphics workstations used as computing nodes are presented. Although a workstation-based distributed processing environment offers many advantages, it also introduces a number of new concerns. In order to solve these problems, allow the environment to function as an integrated system, and present a functional development environment to application programmers, it is necessary to develop an additional layer of software. This 'executive' software integrates the system, provides real-time capabilities, and provides the tools necessary to support the application requirements

Abundance of conserved CRISPR-Cas9 target sites within the highly polymorphic genomes of Anopheles and Aedes mosquitoes.

A number of recent papers report that standing genetic variation in natural populations includes ubiquitous polymorphisms within target sites for Cas9-based gene drive (CGD) and that these "drive resistant alleles" (DRA) preclude the successful application of CGD for managing these populations. Here we report the results of a survey of 1280 genomes of the mosquitoes Anopheles gambiae, An. coluzzii, and Aedes aegypti in which we determine that ~90% of all protein-encoding CGD target genes in natural populations include at least one target site with no DRAs at a frequency of ≥1.0%. We conclude that the abundance of conserved target sites in mosquito genomes and the inherent flexibility in CGD design obviates the concern that DRAs present in the standing genetic variation of mosquito populations will be detrimental to the deployment of this technology for population modification strategies

Assessing the Market for Poultry Litter in Georgia: Are Subsidies Needed to Protect Water Quality?

Concerns about nutrient loads into our waters have focused attention on poultry litter applications. Like many states with a large poultry industry, Georgia recently designed a subsidy program to facilitate the transportation of poultry litter out of vulnerable watersheds. This paper uses a transportation model to examine the necessity of a poultry litter subsidy to achieve water protection goals in Georgia. We also demonstrate the relationship between diesel and synthetic fertilizer prices and the value of poultry litter. Results suggest that a well functioning market would be able to remove excess litter from vulnerable watersheds in the absence of a subsidy.fertilizer, phosphorous, poultry litter, subsidy, transportation model, water quality, Environmental Economics and Policy, Marketing, Q12, Q13, Q25, Q53,

Continuation of research into software for space operations support: Conversion of the display manager to X Windows/Motif, volume 2

NASA is currently using a set of applications called the Display Builder and Display Manager. They run on Concurrent systems and heavily depend on the Graphic Kernel System (GKS). At this time however, these two applications would more appropriately be developed in X Windows, in which a low X is used for all actual text and graphics display and a standard widget set (such as Motif) is used for the user interface. Use of the X Windows will increase performance, improve the user interface, enhance portability, and improve reliability. Prototype of X Window/Motif based Display Manager provides the following advantages over a GKS based application: improved performance by using a low level X Windows, display of graphic and text will be more efficient; improved user interface by using Motif; Improved portability by operating on both Concurrent and Sun workstations; and Improved reliability

Continuation of research into software for space operations support, volume 1

A prototype workstation executive called the Hardware Independent Software Development Environment (HISDE) was developed. Software technologies relevant to workstation executives were researched and evaluated and HISDE was used as a test bed for prototyping efforts. New X Windows software concepts and technology were introduced into workstation executives and related applications. The four research efforts performed included: (1) Research into the usability and efficiency of Motif (an X Windows based graphic user interface) which consisted of converting the existing Athena widget based HISDE user interface to Motif demonstrating the usability of Motif and providing insight into the level of effort required to translate an application from widget to another; (2) Prototype a real time data display widget which consisted of research methods for and prototyping the selected method of displaying textual values in an efficient manner; (3) X Windows performance evaluation which consisted of a series of performance measurements which demonstrated the ability of low level X Windows to display textural information; (4) Convert the Display Manager to X Window/Motif which is the application used by NASA for data display during operational mode

A standardised sampling protocol for robust assessment of reach-scale fish community diversity in wadeable New Zealand streams

The New Zealand fish fauna contains species that are affected not only by river system connectivity, but also by catchment and local-scale changes in landcover, water quality and habitat quality. Consequently, native fish have potential as multi-scale bioindicators of human pressure on stream ecosystems, yet no standardised, repeatable and scientifically defensible methods currently exist for effectively quantifying their abundance or diversity in New Zealand stream reaches. Here we report on the testing of a back-pack electrofishing method, modified from that used by the United States Environmental Protection Agency, on a wide variety of wadeable stream reaches throughout New Zealand. Seventy-three first- to third-order stream reaches were fished with a single pass over 150-345 m length. Time taken to sample a reach using single-pass electrofishing ranged from 1-8 h. Species accumulation curves indicated that, irrespective of location, continuous sampling of 150 stream metres is required to accurately describe reach-scale fish species richness using this approach. Additional species detection beyond 150 m was rare (<10%) with a single additional species detected at only two out of the 17 reaches sampled beyond this distance. A positive relationship was also evident between species detection and area fished, although stream length rather than area appeared to be the better predictor. The method tested provides a standardised and repeatable approach for regional and/or national reporting on the state of New Zealand's freshwater fish communities and trends in richness and abundance over time

Novel pathogenic COX20 variants causing dysarthria, ataxia, and sensory neuropathy.

COX20/FAM36A encodes a mitochondrial complex IV assembly factor important for COX2 activation. Only one homozygous COX20 missense mutation has been previously described in two separate consanguineous families. We report four subjects with features that include childhood hypotonia, areflexia, ataxia, dysarthria, dystonia, and sensory neuropathy. Exome sequencing in all four subjects identified the same novel COX20 variants. One variant affected the splice donor site of intron-one (c.41A&gt;G), while the other variant (c.157+3G&gt;C) affected the splice donor site of intron-two. cDNA and protein analysis indicated that no full-length cDNA or protein was generated. These subjects expand the phenotype associated with COX20 deficiency

The coastal environment and human health : microbial indicators, pathogens, sentinels and reservoirs

© 2008 Author et al. This is an open access article distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Environmental Health 7 (2008): S3, doi:10.1186/1476-069X-7-S2-S3.Innovative research relating oceans and human health is advancing our understanding of disease-causing organisms in coastal ecosystems. Novel techniques are elucidating the loading, transport and fate of pathogens in coastal ecosystems, and identifying sources of contamination. This research is facilitating improved risk assessments for seafood consumers and those who use the oceans for recreation. A number of challenges still remain and define future directions of research and public policy. Sample processing and molecular detection techniques need to be advanced to allow rapid and specific identification of microbes of public health concern from complex environmental samples. Water quality standards need to be updated to more accurately reflect health risks and to provide managers with improved tools for decision-making. Greater discrimination of virulent versus harmless microbes is needed to identify environmental reservoirs of pathogens and factors leading to human infections. Investigations must include examination of microbial community dynamics that may be important from a human health perspective. Further research is needed to evaluate the ecology of non-enteric water-transmitted diseases. Sentinels should also be established and monitored, providing early warning of dangers to ecosystem health. Taken together, this effort will provide more reliable information about public health risks associated with beaches and seafood consumption, and how human activities can affect their exposure to disease-causing organisms from the oceans.The Oceans and Human Health Initiative research described within this paper is supported by the National Science Foundation, The National Institute for Environmental Health Sciences and the National Oceanic and Atmospheric Administration. Grant numbers are: NIEHS P50 ES012742 and NSF OCE- 043072 (RJG, LAA-Z, MFP), NSF OCE04-32479 and NIEHS P50 ES012740 (RSF), NSF OCE-0432368 and NIEHS P50 ES12736 (HMS-G), NIEHS P50 ES012762 and NSF OCE-0434087 (JSM)

Controversies Surrounding Renal Denervation:Lessons Learned From Real-World Experience in Two United Kingdom Centers

Renal denervation (RDN) is a therapy that targets treatment‐resistant hypertension (TRH). The Renal Denervation in Patients With Uncontrolled Hypertension (Symplicity) HTN‐1 and Symplicity HTN‐2 trials reported response rates of >80%; however, sham‐controlled Symplicity HTN‐3 failed to reach its primary blood pressure (BP) outcome. The authors address the current controversies surrounding RDN, illustrated with real‐world data from two centers in the United Kingdom. In this cohort, 52% of patients responded to RDN, with a 13±32 mm Hg reduction in office systolic BP (SBP) at 6 months (n=29, P=.03). Baseline office SBP and number of ablations correlated with office SBP reduction (R=−0.47, P=.01; R=−0.56, P=.002). RDN appears to be an effective treatment for some patients with TRH; however, individual responses are highly variable. Selecting patients for RDN is challenging, with only 10% (33 of 321) of the screened patients eligible for the study. Medication alterations and nonadherence confound outcomes. Adequate ablation is critical and should impact future catheter design/training. Markers of procedural success and improved patient selection parameters remain key research aims