1,724 research outputs found

    Investigating the potential regulation of Asic2 by microRNAs

    Get PDF
    Multiple Sclerosis (MS) is an inflammatory disease which causes neurodegeneration. It is a disease which affects mainly young adults, and symptoms become more aggressive over time. It is thought that mutations in a variety of genes may be a large contributor to the development of MS. Many Linkage and Genome Wide Association Studies have been performed to find genes which may be mis-expressed among different sections of the world’s population. Through one of these Genome Wide Associations a Single Nucleotide Polymorphism (SNP) was found in the 3’ UTR of the ACCN1 gene. This gene codes for Acid Sensing Ion Channel 2, a major ion channel predominantly found in cells within the Central Nervous System (CNS). As this channel is closely connected to systems in the CNS which are known to be affected in patients with MS, it was thought that the SNP should be investigated further. As the SNP was found in the 3’ UTR it was suggested that miRNAs may play a role. This study took on this suggestion and investigated the possibility of ACCN1 being controlled by a miRNA and whether this control was in any way affected by the SNP found. The initial experiment found that of the two variants, rs28936A was controlled more strongly by a miRNA than the variant rs28936G. Resequencing of the samples showed a further three SNPs in the same region. Several miRNA target sites were predicted that contained at least one of the SNPs, however none of these could be validated experimentally using luciferase essays. The three SNPs were tested individually using the same luciferase experiment but none were found to have a more significant effect over luciferase expression over the others. The results therefore indicate that all SNPs contribute to the reduction in miRNA binding. Further research is proposed to investigate in more detail, potential miRNA candidates. It was concluded from these results that the ACCN1 gene is being controlled by an unknown miRNA via binding to the 3’UTR, that the rs28936A variant is more strongly controlled by the miRNA and that of all SNPs found (including the two originals and three ‘new’ SNPs) none have a significantly stronger miRNA control than the other

    Taking Control of Archdesc: Implementing Spaceport for Special Collections Finding Aids

    Get PDF
    This poster describes a project to develop Spaceport, a Python app that transforms EAD finding aids from ArchivesSpace into EADs for Virginia Heritage, HTML files for the JMU Libraries website, and MARCXML records for the catalog. It outlines the challenges and needs that led to Spaceport\u27s development and describes benefits experienced from its implementation

    Concert: The Mary Hayes North Recital Competition

    Get PDF

    Understudied and Underfunded: Potential Causes of Mass Shootings and Implications for Counseling Research

    Get PDF
    Mass shootings are becoming more commonplace in our society. Despite this increase in frequency, research on mass violence has lagged behind due to halted federal research funding. Counselors and other mental health professionals find themselves at the forefront of this issue due to the contentious debate surrounding the intersection of mental health issues and mass shootings. The purpose of this article is to increase awareness of the cause of this dearth of research, examine what is currently known in the limited scholarly literature, and discuss what still needs to be explored. Implications for positive social change and advocacy efforts are also provided

    Counseling Active-Duty Female Service Members who have Experienced Sexual Assault: A Guide for Professional Counselors

    Get PDF
    Sexual assault is a prevailing problem for active duty women in the United States military. We explore the unique nature of military culture and explain the military’s dual pathway process for reporting sexual assault. In addition, we describe, in depth, evidence-based theoretical approaches applicable for counselors to use in their work with female service members who are survivors of sexual assault. Finally, we discuss sexual assault resources available to military clients and the steps counselors can take to engage in advocacy for and with this population

    School based sexual abuse prevention: Implications for professional school counselors

    Get PDF
    The purpose of this qualitative collective case study was to explore the experiences of four key internal stakeholders who are involved with a school-based child sexual abuse prevention program in a southeastern state in the United States. In order to explore the experiences of participants, in-depth, semistructured interviews were conducted. After the data were collected, transcribed, and coded by a qualified research team, three main themes emerged from this study. These themes highlighted the importance of school-based child sexual abuse prevention education, various program impacts resulting from child sexual abuse prevention and intervention within a school setting, and barriers to implementation of child sexual abuse prevention programs in school settings. Implications for professional school counselors, including how they can assist with the implementation of child sexual abuse prevention education, are discussed. Finally, inherent limitations to the research design and implications for future studies are addressed
    • …
    corecore