727 research outputs found
The small FNR regulon of Neisseria gonorrhoeae: comparison with the larger Escherichia coli FNR regulon and interaction with the NarQ-NarP regulon
BACKGROUND: Neisseria gonorrhoeae can survive during oxygen starvation by reducing nitrite to nitrous oxide catalysed by the nitrite and nitric oxide reductases, AniA and NorB. The oxygen-sensing transcription factor, FNR, is essential for transcription activation at the aniA promoter, and full activation also requires the two-component regulatory system, NarQ-NarP, and the presence of nitrite. The only other gene known to be activated by the gonococcal FNR is ccp encoding a cytochrome c peroxidase, and no FNR-repressed genes have been reported in the gonococcus. In contrast, FNR acts as both an activator and repressor involved in the control of more than 100 operons in E. coli regulating major changes in the adaptation from aerobic to anaerobic conditions. In this study we have performed a microarray-led investigation of the FNR-mediated responses in N. gonorrhoeae to determine the physiological similarities and differences in the role of FNR in cellular regulation in this species. RESULTS: Microarray experiments show that N. gonorrhoeae FNR controls a much smaller regulon than its E. coli counterpart; it activates transcription of aniA and thirteen other genes, and represses transcription of six genes that include dnrN and norB. Having previously shown that a single amino acid substitution is sufficient to enable the gonococcal FNR to complement an E. coli fnr mutation, we investigated whether the gonococcal NarQ-NarP can substitute for E. coli NarX-NarL or NarQ-NarP. A plasmid expressing gonococcal narQ-narP was unable to complement E. coli narQP or narXL mutants, and was insensitive to nitrate or nitrite. Mutations that progressively changed the periplasmic nitrate sensing region, the P box, of E. coli NarQ to the sequence of the corresponding region of gonococcal NarQ resulted in loss of transcription activation in response to the availability of either nitrate or nitrite. However, the previously reported ligand-insensitive ability of gonococcal NarQ, the "locked on" phenotype, to activate either E. coli NarL or NarP was confirmed. CONCLUSION: Despite the sequence similarities between transcription activators of E. coli and N. gonorrhoeae, these results emphasise the fundamental differences in transcription regulation between these two types of pathogenic bacteria
Pan American Climate Study (PACS) mooring deployment cruise report : R/V Roger Revelle cruise number Genesis 4, 9 April-5 May 1997
Three surface moorings were deployed in the eastern equatorial Pacifc from the R/V Roger Revelle as part of the Pan American Climate
Study (PACS). PACS is a NOAA-funded study with the goal of investigating links between sea surface temperature varabilty in the tropical
oceans near the Americas and climate over the American continents. The three moorings were deployed near 125°W, spanning the strong
meridional sea surface temperature gradient associated with the cold tongue south of the equator and the warmer ocean north of the equator,
near the northernmost, summer location of the Intertropical Convergence Zone. The mooring deployment was done to improve
understading of the air-sea fluxes and of the processes that control the evolution of the sea surface temperature field in the region.
Two surface moorings of the Upper Ocean Processes Group at the Woods Hole Oceanographic Institution (WHOI) were deployed-one at
3°S, 125°W and the other at lO°N, 125°W. One mooring from the Ocean Circulation Group (R. Weisberg) at the University of South Florida
(USP) was deployed on the equator at 128°W.
The buoys of the two WHOI moorings were each equipped with meteorological instrmentation, including a Vector Averaging Wind
Recorder, and an Improved Meteorological (IMET) system. The WHOI moorings also carried Vector Measurng Current Meters,
single-point temperature recorders, and conductivity and temperature recorders located in the upper 200 meters of the mooring line. In
addition to the instrumentation noted above, a variety of other instruments, including an acoustic current meter, acoustic doppler current
meters, bio-optical instrument packages and an acoustic rain gauge, were deployed during the PACS field program. The USF mooring had an
IMET system on the surface buoy and for oceanographic instrumentation, two RD Instruments acoustic doppler current profilers,
single-point temperature recorders, and conductivity and temperature recorders. Conductivity-temperature-depth (CTD) profiles were made
at each mooring site and during the transit between mooring locations.
This report describes, in a general manner, the work that took place durig the Genesis 4 cruise aboard the R/V Roger Revelle. The three
surface moorings deployed during this cruise will be recovered and re-deployed after approximately nine months, with a final recovery
planned for 17 months after the first setting. Details of the mooring designs and preliminary data from the CT profies are included.Funding was provided by the National Oceanic and Atmospheric Administration under
Contract No. NA66GP0130
Stellar Populations at the Center of IC 1613
We have observed the center of the Local Group dwarf irregular galaxy IC 1613
with WFPC2 aboard the Hubble Space Telescope in the F439W, F555W, and F814W
filters. We find a dominant old stellar population (aged ~7 Gyr), identifiable
by the strong red giant branch (RGB) and red clump populations. From the (V-I)
color of the RGB, we estimate a mean metallicity of the intermediate-age
stellar population [Fe/H] = -1.38 +/- 0.31. We confirm a distance of 715 +/- 40
kpc using the I-magnitude of the RGB tip. The main-sequence luminosity function
down to I ~25 provides evidence for a roughly constant SFR of approximately
0.00035 solar masses per year across the WFPC2 field of view (0.22 square kpc)
during the past 250-350 Myr. Structure in the blue loop luminosity function
implies that the SFR was ~50% higher 400-900 Myr ago than today. The mean heavy
element abundance of these young stars is 1/10th solar. The best explanation
for a red spur on the main-sequence at I = 24.7 is the blue horizontal branch
component of a very old stellar population at the center of IC 1613. We have
also imaged a broader area of IC 1613 using the 3.5-meter WIYN telescope under
excellent seeing conditions. The AGB-star luminosity function is consistent
with a period of continuous star formation over at least the age range 2-10
Gyr. We present an approximate age-metallicity relation for IC 1613, which
appears similar to that of the Small Magellanic Cloud. We compare the Hess
diagram of IC 1613 to similar data for three other Local Group dwarf galaxies,
and find that it most closely resembles the nearby, transition-type dwarf
galaxy Pegasus (DDO 216).Comment: To appear in the September 1999 Astronomical Journal. LaTeX, uses
AASTeX v4.0, emulateapj style file, 19 pages, 12 postscript figures, 2
tables. 5 of the figures available separately via the WW
Observations and Implications of the Star Formation History of the LMC
We present derivations of star formation histories based on color-magnitude
diagrams of three fields in the LMC from HST/WFPC2 observations. A significant
component of stars older than 4 Gyr is required to match the observed
color-magnitude diagrams. Models with a dispersion-free age-metallicity
relation are unable to reproduce the width of the observed main sequence;
models with a range of metallicity at a given age provide a much better fit.
Such models allow us to construct complete ``population boxes'' for the LMC
based entirely on color-magnitude diagrams; remarkably, these qualitatively
reproduce the age-metallicity relation observed in LMC clusters. We discuss
some of the uncertainties in deriving star formation histories. We find,
independently of the models, that the LMC bar field has a larger relative
component of older stars than the outer fields. The main implications suggested
by this study are: 1) the star formation history of field stars appears to
differ from the age distribution of clusters, 2) there is no obvious evidence
for bursty star formation, but our ability to measure bursts shorter in
duration than 25% of any given age is limited by the statistics of the
observed number of stars, 3) there may be some correlation of the star
formation rate with the last close passage of the LMC/SMC/Milky Way, but there
is no dramatic effect, and 4) the derived star formation history is probably
consistent with observed abundances, based on recent chemical evolution models.Comment: Accepted by AJ, 36 pages including 12 figure
Stellar Populations in Three Outer Fields of the LMC
We present HST photometry for three fields in the outer disk of the LMC
extending approximately four magnitudes below the faintest main sequence
turnoff. We cannot detect any strongly significant differences in the stellar
populations of the three fields based on the morphologies of the
color-magnitude diagrams, the luminosity functions, and the relative numbers of
stars in different evolutionary stages. Our observations therefore suggest
similar star formation histories in these regions, although some variations are
certainly allowed. The fields are located in two regions of the LMC: one is in
the north-east field and two are located in the north-west. Under the
assumption of a common star formation history, we combine the three fields with
ground-based data at the same location as one of the fields to improve
statistics for the brightest stars. We compare this stellar population with
those predicted from several simple star formation histories suggested in the
literature, using a combination of the R-method of Bertelli et al (1992) and
comparisons with the observed luminosity function. The only model which we
consider that is not rejected by the observations is one in which the star
formation rate is roughly constant for most of the LMC's history and then
increases by a factor of three about 2 Gyr ago. Such a model has roughly equal
numbers of stars older and younger than 4 Gyr, and thus is not dominated by
young stars. This star formation history, combined with a closed box chemical
evolution model, is consistent with observations that the metallicity of the
LMC has doubled in the past 2 Gyr.Comment: 30 pages, includes 10 postscript figures. Figure 1 avaiable at
ftp://charon.nmsu.edu/pub/mgeha/LMC. Accepted for publication in Astronomical
Journa
The WFC3 Galactic Bulge Treasury Program: A First Look at Resolved Stellar Population Tools
[Abridged] When WFC3 is installed on HST, the community will have powerful
new tools for investigating resolved stellar populations. The WFC3 Galactic
Bulge Treasury program will obtain deep imaging on 4 low-extinction fields.
These non-proprietary data will enable a variety of science investigations not
possible with previous data sets. To aid in planning for the use of these data
and for future proposals, we provide an introduction to the program, its
photometric system, and the associated calibration effort.
The observing strategy is based upon a new 5-band photometric system spanning
the UV, optical, and near-infrared. With these broad bands, one can construct
reddening-free indices of Teff and [Fe/H]. Besides the 4 bulge fields, the
program will target 6 fields in well-studied star clusters, spanning a wide
range of [Fe/H]. The cluster data serve to calibrate the indices, provide
population templates, and correct the transformation of isochrones into the
WFC3 photometric system. The bulge data will shed light on the bulge formation
history, and will also serve as population templates for other studies. One of
the fields includes 12 candidate hosts of extrasolar planets.
CMDs are the most popular tool for analyzing resolved stellar populations.
However, due to degeneracies among Teff, [Fe/H], and reddening in traditional
CMDs, it can be difficult to draw robust conclusions from the data. The 5-band
system used for the bulge Treasury observations will provide indices that are
roughly orthogonal in Teff and [Fe/H], and we argue that model fitting in an
index-index diagram will make better use of the information than fitting
separate CMDs. We provide simulations to show the expected data quality and the
potential for differentiating between different star-formation histories.Comment: Accepted for publication in The Astronomical Journal. 9 pages, 8
figures, latex, AJ forma
Surgical stabilization versus nonoperative treatment for flail and non-flail rib fracture patterns in patients with traumatic brain injury
Purpose
Literature on outcomes after SSRF, stratified for rib fracture pattern is scarce in patients with moderate to severe traumatic brain injury (TBI; Glasgow Coma Scale ≤ 12). We hypothesized that SSRF is associated with improved outcomes as compared to nonoperative management without hampering neurological recovery in these patients. Methods
A post hoc subgroup analysis of the multicenter, retrospective CWIS-TBI study was performed in patients with TBI and stratified by having sustained a non-flail fracture pattern or flail chest between January 1, 2012 and July 31, 2019. The primary outcome was mechanical ventilation-free days and secondary outcomes were in-hospital outcomes. In multivariable analysis, outcomes were assessed, stratified for rib fracture pattern. Results
In total, 449 patients were analyzed. In patients with a non-flail fracture pattern, 25 of 228 (11.0%) underwent SSRF and in patients with a flail chest, 86 of 221 (38.9%). In multivariable analysis, ventilator-free days were similar in both treatment groups. For patients with a non-flail fracture pattern, the odds of pneumonia were significantly lower after SSRF (odds ratio 0.29; 95% CI 0.11–0.77; p = 0.013). In patients with a flail chest, the ICU LOS was significantly shorter in the SSRF group (beta, − 2.96 days; 95% CI − 5.70 to − 0.23; p = 0.034). Conclusion
In patients with TBI and a non-flail fracture pattern, SSRF was associated with a reduced pneumonia risk. In patients with TBI and a flail chest, a shorter ICU LOS was observed in the SSRF group. In both groups, SSRF was safe and did not hamper neurological recovery
Far-Ultraviolet Imaging of the Large Magellanic Cloud Populous Cluster NGC 1978 with WFPC2
We have imaged the ~2.2 billion-year-old Large Magellanic Cloud populous cluster NGC 1978 in the far-ultraviolet and visible with the second Wide Field/Planetary Camera (WFPC2) on the Hubble Space Telescope. The far-ultraviolet images show a sparse stellar field with little apparent density enhancement in the cluster core. The visible images are dominated by the cluster's first-ascent and second-ascent red giants, which are completely invisible to the far-ultraviolet filter. No evidence for a hot horizontal branch population of core-helium-burning stars is seen; nor is there any apparent indication of a significant blue
straggler population. These results suggest that the presence of a rich, young population of field stars in the
NGC 1978 region is responsible for the unusual location of the cluster in the integrated light color-color plots produced by IUE
Unusual raptor nests around the world
From surveys in many countries, we report using unusual nesting materials (e.g., paper money, rags, metal, antlers, and large bones) and unusual nesting situations. For example, we documented nests of Steppe Eagles [Aquila nipalensis] and Upland Buzzards [Buteo hemilasius] on the ground beside well-traveled roads, Saker Falcon [Falco cherrug] eyries in attics and a cistern, and Osprey [Pandian haliaetus] nests on the masts of boats and on a suspended automobile. Other records include a Golden Eagle [A. chrysaelos] nest 7.0 m in height, believed to be the tallest nest ever described, and, for the same species, we report nesting in rudimentary, nests. Some nest sites are within a Few meters of known predators or competitors. These unusual observations may be important in revealing the plasticity of a species' behavioral repertoire
Detection of the Power Spectrum of Cosmic Microwave Background Lensing by the Atacama Cosmology Telescope
We report the first detection of the gravitational lensing of the cosmic
microwave background through a measurement of the four-point correlation
function in the temperature maps made by the Atacama Cosmology Telescope. We
verify our detection by calculating the levels of potential contaminants and
performing a number of null tests. The resulting convergence power spectrum at
2-degree angular scales measures the amplitude of matter density fluctuations
on comoving length scales of around 100 Mpc at redshifts around 0.5 to 3. The
measured amplitude of the signal agrees with Lambda Cold Dark Matter cosmology
predictions. Since the amplitude of the convergence power spectrum scales as
the square of the amplitude of the density fluctuations, the 4-sigma detection
of the lensing signal measures the amplitude of density fluctuations to 12%.Comment: 4 pages, 4 figures, replaced title and author list with version
accepted by Physical Review Letters. Likelihood code can be downloaded from
http://bccp.lbl.gov/~sudeep/ACTLensLike.htm
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