Spatio-temporal modelling of weekly malaria incidence in children under 5 for early epidemic detection in Mozambique

Malaria is a major cause of morbidity and mortality in Mozambique. We present a malaria early warning system (MEWS) for Mozambique informed by seven years of weekly case reports of malaria in children under 5 years of age from 142 districts. A spatio-temporal model was developed based on explanatory climatic variables to map exceedance probabilities, defined as the predictive probability that the relative risk of malaria incidence in a given district for a particular week will exceed a predefined threshold. Unlike most spatially discrete models, our approach accounts for the geographical extent of each district in the derivation of the spatial covariance structure to allow for changes in administrative boundaries over time. The MEWS can thus be used to predict areas that may experience increases in malaria transmission beyond expected levels, early enough so that prevention and response measures can be implemented prior to the onset of outbreaks. The framework we present is also applicable to other climate-sensitive diseases

Testosterone metabolism in Neomysis integer following exposure to benzo(a)pyrene

Author Posting. © Elsevier B.V., 2006. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 144 (2006): 405-412, doi:10.1016/j.cbpb.2006.04.001.Cytochromes P450 (CYPs) are important enzymes involved in the regulation of hormone synthesis and in the detoxification and/or activation of xenobiotics. CYPs are found in virtually all organisms, from archae, and eubacteria to eukaryota. A number of endocrine disruptors are suspected of exerting their effects through disruption of normal CYP function. Consequently, alterations in steroid hormone metabolism through changes in CYP could provide an important tool to evaluate potential effects of endocrine disruptors. The aim of this study was to investigate the potential effects of the known CYP modulator, benzo(a)pyrene (B(a)P), on the testosterone metabolism in the invertebrate Neomysis integer (Crustacea; Mysidacea). N. integer were exposed for 96h to 0.43, 2.39, 28.83, 339.00 and 1682.86μg B(a)P L-1 and a solventcontrol, and subsequently their ability to metabolize testosterone was assessed. Identification and quantification of the produced phase I and phase II testosterone metabolites was performed using liquid chromatography coupled with multiple mass spectrometry (LC-MS2). Significant changes were observed in the overall ability of N. integer to metabolize testosterone when exposed to 2.39, 28.83, 339.00 and 1682.86μg B(a)P L-1 as compared to the control animals.This research was supported by a research grant of the Ghent University Research Fund (BOF, 011.072.02). Dr. Tim Verslycke was supported by a Postdoctoral Fellowship of the Belgian American Educational Foundation

Acute Hepatitis B Outbreaks in 2 Skilled Nursing Facilities and Possible Sources of Transmission: North Carolina, 2009–2010

Objective. Acute hepatitis B virus (HBV) infections have been reported in long-term care facilities (LTCFs), primarily associated with infection control breaks during assisted blood glucose monitoring. We investigated HBV outbreaks that occurred in separate skilled nursing facilities (SNFs) to determine factors associated with transmission. Design. Outbreak investigation with case-control studies. Setting. Two SNFs (facilities A and B) in Durham, North Carolina, during 2009–2010. Patients. Residents with acute HBV infection and controls randomly selected from HBV-susceptible residents during the outbreak period. Methods. After initial cases were identified, screening was offered to all residents, with repeat testing 3 months later for HBV-susceptible residents. Molecular testing was performed to assess viral relatedness. Infection control practices were observed. Case-control studies were conducted to evaluate associations between exposures and acute HBV infection in each facility. Results. Six acute HBV cases were identified in each SNF. Viral phylogenetic analysis revealed a high degree of HBV relatedness within, but not between, facilities. No evaluated exposures were significantly associated with acute HBV infection in facility A; those associated with infection in facility B (all odds ratios >20) included injections, hospital or emergency room visits, and daily blood glucose monitoring. Observations revealed absence of trained infection control staff at facility A and suboptimal hand hygiene practices during blood glucose monitoring and insulin injections at facility B. Conclusions. These outbreaks underscore the vulnerability of LTCF residents to acute HBV infection, the importance of surveillance and prompt investigation of incident cases, and the need for improved infection control education to prevent transmission

Identifying and Quantifying Genotypes in Polyclonal Infections due to Single Species

The combination of real-time PCR and capillary electrophoresis permits the rapid identification and quantification of pathogen genotypes

Beyond Environmental Regulatory Fragmentation: Signs of Integration in the Case of the Great Lakes Basin

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/72007/1/j.1468-0491.1995.tb00197.x.pd

Status of the Keck Planet Imager and Characterizer phase II development

The Keck Planet Imager and Characterizer comprises of a series of upgrades to the Keck II adaptive optics system and instrument suite to improve the direct imaging and high resolution spectroscopy capabilities of the facility instruments NIRC2 and NIRSPEC, respectively. Phase I of KPIC includes a NIR pyramid wavefront sensor and a Fiber Injection Unit (FIU) to feed NIRSPEC with a single mode fiber, which have already been installed and are currently undergoing commissioning. KPIC will enable High Dispersion Coronagraphy (HDC) of directly imaged exoplanets for the first time, providing potentially improved detection significance and spectral characterization capabilities compared to direct imaging. In favorable cases, Doppler imaging, spin measurements, and molecule mapping are also possible. This science goal drives the development of phase II of KPIC, which is scheduled to be deployed in early 2020. Phase II optimizes the system throughput and contrast using a variety of additional submodules, including a 952 element deformable mirror, phase induced amplitude apodization lenses, an atmospheric dispersion compensator, multiple coronagraphs, a Zernike wavefront sensor, and multiple science ports. A testbed is being built in the Exoplanet Technology Lab at Caltech to characterize and test the design of each of these submodules before KPIC phase II is deployed to Keck. This paper presents an overview of the design of phase II and report on results from laboratory testing