Allozyme variation in Rattus rattus (Rodentia: Muridae) in Turkey, with particular emphasis on the taxonomy

WOS: 000261906600003The Turkish black rat "Rattus rattus" shows variation in coat colour corresponding to the occurrence of three subspecies with intermediate colour stages: Rattus rattus rattus, Rattus r. alexandrinus and Rattus r. frugivorus. Turkish black rat populations were divided geographically into six sub-populations: Rr1 = Northwest Anatolia, Rr2 = Central Anatolia, Rr3 = Eastern Mediterranean, Rr4 = Western Mediterranean, Rr5 = Turkish Thrace, and Rr6 = Black Sea region. Genetic variation was assessed using twenty two isoenzyme systems. Seven of twenty-two loci (Pgm-1, Hk, Ale-M, G3pdh, Gpdh-1, Gpi, Fum-1) were found to be polymorphic. The mean Value of F(ST) is found to be 0.073, indicating 7.3 % genetic variation among groups and suggesting the existence of a moderate differentiation between sub-populations of the Turkish black rat. Overall mean heterozygosity (Ho = direct count) for sub-populations was Ho = 0.020, ranging from 0.008 to 0.031. Nei's measure of genetic distance showed that Rr2 and Rr6 were the most identical and sub-populations Rr1 and Rr5 had diverged the most.BAP of Ankara UniversityAnkara University [97.05.03.04, 2000.07.05.037]We wish to thank Dr Ben BRILOT for comments on the text. This study was supported by BAP (97.05.03.04 and 2000.07.05.037) of Ankara University

Genetic differentiation of the Meriones tristrami (Mammalia: Rodentia) subpopulations in Turkey - inferring allozyme variations

WOS: 000393615100007Allozyme variations of Tristram's jird Meriones tristrami, which is distributed in the steppe and semiarid areas of Turkey, were studied using 24 loci of 83 specimens from ten locations in Turkey. Seven loci were found to be polymorphic. According to the patterns of allozyme variation, the percentage of polymorphic loci was 12.5. F-ST was found to be 0.44, indicating high genetic variations among M. tristrami; accordingly the Nm value (0.3157) appeared quite low. The UPGMA dendrogram, based on genetic distance, showed that the populations established two very close subclusters, and supported the idea that the westernmost population connected to the northern part of the central Anatolian population. As expected, the eastern population appeared to be more divergent due to geographic distance. Our findings supported that genetic isolation among these subpopulations might be caused by geographic isolation.Ankara University-BAP (TUBITAK, Turkey) [09H4240003, TBAG-107T324]This research was financially support by Ankara University-BAP 09H4240003 and TBAG-107T324 (TUBITAK, Turkey)

On the karyotype of the Long-clawed mole vole, Prometheomys schaposchnikovi Satunin, 1901 (Mammalia: Rodentia), in Turkey

Volume: 64Start Page: 126End Page: 12

Allozyme Variation in Wild Rats Rattus norvegicus (BERICENHOUT 1769) (Mammalia: Rodentia) from Turkey

WOS: 000277433300010The genetic diversity of 22 allozyme loci was investigated in 33 wild rat (Rattus norvegicus) specimens from 4 sub-populations. Eight of the 22 loci (Pgm, Hk, Me-M, Ldh, alpha-Gpdh-1, alpha-Gpdh-2, Fum, Xdh) were polymorphic. The level of genetic variation was measured by mean number of alleles per locus (A = 1.18), percentage of polymorphic loci (P = 18.2), mean heterozygosity per locus observed (Ho = 0.007), and mean heterozygosity expected under Hardy - Weinberg equilibrium (He = 0.057). Mean Psi, value was 0.34, indicating 34% genetic variation and suggesting high-level differentiation between sub-populations of the Turkish wild rat. The number of migrants (Nm) was 0.48 and showed that gene flow was relatively low between sub-populations. Even though a high level of genetic variation was observed, the low Nm value and Ho could be evidence of the Wahlund effect or a genetic bottleneck for the sub-populations. It is also suspected that there might be some factors preventing gene flow from the sub-populations, which constitute their own genetic potential for new taxonomic units

Allozyme variation in bank vole, Myodes glareolus (Mammalia: Rodentia) in Northern Anatolia

WOS: 000325597600045A total of 94 specimens from 16 populations of Myodes glareolus, collected between 2004 and 2007, from different altitudinal distributions were analyzed, using 16 enzyme systems. We found that 10 out of 22 loci (Idh-2, alpha-Gpdh, Me, Pgm, Pgd, Mdh-s, Ada, Est-1, Ldh-1, and Ldh-2) were polymorphic. Group 1 included population from altitudes ranging from 27 to 605 m above sea level (ASL), and Group 2 were from altitudes ranging from 1003 to 1288 m ASL. The summaries of the genetic parameters also displayed differences between the 2 groups. The possible reasons of such fragmentation between M. glareolus populations were discussed. (C) 2013 Elsevier Ltd. All rights reserved.BAPRO of Ankara UniversityAnkara University [2003-07-05-077]This study was sponsored by BAPRO (NO: 2003-07-05-077) of Ankara University

Taxonomic status of the genus Spermophilus (Mammalia : Rodentia) in Turkey and Iran with description of a new species

WOS: 000248167000001We report a new species of Spermophilus ( Rodentia: Sciuridae), here designated as S. torosensis sp. nov., distributed in the Taurus Mountains in southern Anatolia, Turkey. A total of 161 specimens of the genus Spermophilus from Turkey and Iran were analyzed for their morphological, morphometric, and karyological characteristics. Uni- and multi-variate statistical analyses of morphologic data for 95 adult specimens yielded 4 distinct groups. Taxonomic evaluations classified the specimens into 4 species Spermophilus citellus, S. xanthopymnus, S. fulvus and S. torosensis sp. nov. Morphometrics, coat coloration, a brush-shaped tail, and a NFa value of 72 are diagnostic characters that distinguish S. torosensis sp. nov. from the other species. In Turkey, S. torosensis sp. nov. was found in 6 locations. The karyotype of S. fulvus also is described for the first time as 2n= 36, NFa= 70 and NFa= 66; new karyotypic data is reported for S. xanthoprymnus from Iran and Turkey

Association Of Hla-B27, Mefv Gene Mutations, Erap1, Il12B And Il23R Gene Polymorphisms With Ankylosing Spondylitis

Objective: Genetic factors have an important role in the pathogenesis of ankylosing spondylitis (AS). The aim of this study was to analyse the association of HLA-B27, MEFV mutations, IL12B, IL23R and ERAP1 polymorphisms in Turkish patients with ankylosing spondylitis. Methods: One hundred AS patients and 100 healthy controls were examined for HLA-B27, 12 common MEFV mutations, IL12B (rs3213120), IL23R (rs11209026), and ERAP1 (rs30187) polymorphisms (SNPs) by allele specific PCR, revers hybridization and sequencing techniques. Bath Ankylosing Spondylitis Disease Activity Index (BASDAI) and Bath Ankylosing Spondylitis Functional Index (BASFI) scores were calculated. Results: Our results confirmed that HLA-B27 was strongly associated with AS (69% vs 7% in controls) (p<0.001, OR: 29.6, 95% CI: 12.3-71.1). We also found an association between uveitis and HLA-B27 positivity in AS patients (p=0.004). The MEFV mutations were significantly frequent in AS patients (40%) compared with healthy controls (22%) (p=0.006, OR: 2.56, 95% CI: 1.3-4.4). We found that ERAP1 rs30187 was significantly associated with AS patients (p=0.033). The rs30187 CT genotype was associated with increased AS risk compared to CC or TT genotypes (OR: 2.1, 95% CI: 1.2-3.7). However, in patients with AS carrying the C allele increased the risk 1.9 times (95% Cl: 1.1-3.3). There was no association with AS and IL12B (rs3213120) and IL23R (rs11209026). There were no significant differences between HLA-B27, MEFV mutations, ERAP1 (rs30187) and Bath AS Disease Activity Index (BASDAI), Bath AS Functional Index (BASFI) scores. Conclusion: This study showed that HLA-B27, MEFV mutations and ERAP1 (rs30187) are AS genetic susceptibility genes. Interactions between ERAP1 and HLA-B27 and MEFV mutations may play an important role in the AS pathogenesis.WoSScopusTr-Dizi

Contributions to the karyology and taxonomy of the genus Spalax G\ufcldenstaedt, 1770 (Mammalia: Rodentia) in Turkey

Volume: 64Start Page: 210End Page: 21