The isolated muscle fibre as a model of disuse atrophy: characterization using PhAct, a method to quantify f-actin

Research into muscle atrophy and hypertrophy is hampered by limitations of the available experimental models. Interpretation of in vivo experiments is confounded by the complexity of the environment while in vitro models are subject to the marked disparities between cultured myotubes and the mature myofibres of living tissues. Here we develop a method (PhAct) based on ex vivo maintenance of the isolated myofibre as a model of disuse atrophy, using standard microscopy equipment and widely available analysis software, to measure f-actin content per myofibre and per nucleus over two weeks of ex vivo maintenance. We characterize the 35% per week atrophy of the isolated myofibre in terms of early changes in gene expression and investigate the effects on loss of muscle mass of modulatory agents, including Myostatin and Follistatin. By tracing the incorporation of a nucleotide analogue we show that the observed atrophy is not associated with loss or replacement of myonuclei. Such a completely controlled investigation can be conducted with the myofibres of a single muscle. With this novel method we can identify those features and mechanisms of atrophy and hypertrophy that are intrinsic to the muscle fibre from those that include activities of other tissues and systemic agents

Safe use of symbols in handover documentation for medical teams

Concern has been reported about the safe use of medical abbreviations in documents such as handover sheets and medical notes, especially when information is being communicated between staff of different specialties (BBC 2008, Sheppard et al. 2008). This article describes a study to investigate whether the use of symbols in handover documentation that is shared within and between multidisciplinary teams (MDTs) has similar safety implications. We asked 19 healthcare professionals from a range of specialties to identify 45 different combinations of 38 individual symbols. The symbols and combinations of symbols were extracted from 102 handover sheets taken from 6 different healthcare contexts in 4 London hospitals. Three symbols proposed in Microsoft's Common User Interface guidelines for alert symbols were also included. Results reveal that while some symbols are well understood, many others are either ambiguous or unknown. These results have implications for the safe use of symbols in medical documents, including paper and electronic handover documents and Electronic Patient Records (EPRs), especially where teams comprise individuals from different professional backgrounds, i.e. MDTs. We offer initial suggestions for standardisation and further research

Use of Dog Parks and the Contribution to Physical Activity for Their Owners

This study described the use of dog parks in several diverse locations and explored the contribution dog parks made to physical activity of the dog owners

Chandra HETGS Multiphase Spectroscopy Of The Young Magnetic O Star Theta(1) Orionis C

We report on four Chandra grating observations of the oblique magnetic rotator theta(1) Ori C (O5.5 V), covering a wide range of viewing angles with respect to the star\u27s 1060 G dipole magnetic field. We employ line-width and centroid analyses to study the dynamics of the X-ray - emitting plasma in the circumstellar environment, as well as line-ratio diagnostics to constrain the spatial location, and global spectral modeling to constrain the temperature distribution and abundances of the very hot plasma. We investigate these diagnostics as a function of viewing angle and analyze them in conjunction with new MHD simulations of the magnetically channeled wind shock mechanism on theta(1) Ori C. This model fits all the data surprisingly well, predicting the temperature, luminosity, and occultation of the X-ray - emitting plasma with rotation phase

Genome-wide sequence and functional analysis of early replicating DNA in normal human fibroblasts

BACKGROUND: The replication of mammalian genomic DNA during the S phase is a highly coordinated process that occurs in a programmed manner. Recent studies have begun to elucidate the pattern of replication timing on a genomic scale. Using a combination of experimental and computational techniques, we identified a genome-wide set of the earliest replicating sequences. This was accomplished by first creating a cosmid library containing DNA enriched in sequences that replicate early in the S phase of normal human fibroblasts. Clone ends were then sequenced and aligned to the human genome. RESULTS: By clustering adjacent or overlapping early replicating clones, we identified 1759 "islands" averaging 100 kb in length, allowing us to perform the most detailed analysis to date of DNA characteristics and genes contained within early replicating DNA. Islands are enriched in open chromatin, transcription related elements, and Alu repetitive elements, with an underrepresentation of LINE elements. In addition, we found a paucity of LTR retroposons, DNA transposon sequences, and an enrichment in all classes of tandem repeats, except for dinucleotides. CONCLUSION: An analysis of genes associated with islands revealed that nearly half of all genes in the WNT family, and a number of genes in the base excision repair pathway, including four of ten DNA glycosylases, were associated with island sequences. Also, we found an overrepresentation of members of apoptosis-associated genes in very early replicating sequences from both fibroblast and lymphoblastoid cells. These data suggest that there is a temporal pattern of replication for some functionally related genes

The OPERA trial : a protocol for the process evaluation of a randomised trial of an exercise intervention for older people in residential and nursing accommodation

Background: The OPERA trial is large cluster randomised trial testing a physical activity intervention to address depression amongst people living in nursing and residential homes for older people. A process evaluation was commissioned alongside the trial and we report the protocol for this process evaluation. Challenges included the cognitive and physical ability of the participants, the need to respect the privacy of all home residents, including study non-participants, and the physical structure of the homes. Evaluation activity had to be organised around the structured timetable of homes, leaving limited opportunities for data collection. The aims of this process evaluation are to provide findings that will assist in the interpretation of the clinical trial results, and to inform potential implementation of the physical activity intervention on a wider scale. Methods/design: Quantitative data on recruitment of homes and individuals is being collected. For homes in the intervention arm, data on dose and fidelity of the intervention delivered; including individual rates of participation in exercise classes are collected. In the control homes, uptake and delivery of depression awareness training is monitored. These data will be combined with qualitative data from an in-depth study of a purposive sample of eight homes (six intervention and two control). Discussion: Although process evaluations are increasingly funded alongside trials, it is still rare to see the findings published, and even rarer to see the protocol for such an evaluation published. Process evaluations have the potential to assist in interpreting and understanding trial results as well as informing future roll-outs of interventions. If such evaluations are funded they should also be reported and reviewed in a similar way to the trial outcome evaluation

Chandra HETGS Multi-Phase Spectroscopy of the Young Magnetic O Star theta^1 Orionis C

We report on four Chandra grating observations of the oblique magnetic rotator theta^1 Ori C (O5.5 V) covering a wide range of viewing angles with respect to the star's 1060 G dipole magnetic field. We employ line-width and centroid analyses to study the dynamics of the X-ray emitting plasma in the circumstellar environment, as well as line-ratio diagnostics to constrain the spatial location, and global spectral modeling to constrain the temperature distribution and abundances of the very hot plasma. We investigate these diagnostics as a function of viewing angle and analyze them in conjunction with new MHD simulations of the magnetically channeled wind shock mechanism on theta^1 Ori C. This model fits all the data surprisingly well, predicting the temperature, luminosity, and occultation of the X-ray emitting plasma with rotation phase.Comment: 52 pages, 14 figures (1 color), 6 tables. To appear in the Astrophysical Journal, 1 August 2005, v628, issue 2. New version corrects e-mail address, figure and table formatting problem

Report from the National Society of Genetic Counselors Service Delivery Model Task Force: A Proposal to Define Models, Components, and Modes of Referral

The Service Delivery Model Task Force (SDMTF) was appointed in 2009 by the leadership of the National Society of Genetic Counselors (NSGC) with a charge to research and assess the capacity of all existing service delivery models to improve access to genetic counseling services in the context of increasing demand for genetic testing and counseling. In approaching this charge, the SDMTF found that there were varying interpretations of what was meant by “service delivery models” and the group held extensive discussions about current practices to arrive at consensus of proposed definitions for current genetic service delivery models, modes of referral and components of service delivery. The major goal of these proposed definitions is to allow for conversations to begin to address the charge to the committee. We propose that current models of service delivery can be defined by: 1) the methods in which genetic counseling services are delivered (In‐person, Telephone, Group and Telegenetics), 2) the way they are accessed by patients (Traditional referral, Tandem, Triage, Rescue and Self‐referral) and 3) the variable components that depend upon multiple factors unique to each service setting. This report by the SDMTF provides a starting point whereby standardized terminology can be used in future studies that assess the effectiveness of these described models to overcome barriers to access to genetic counseling services.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/146876/1/jgc40645.pd

X-Ray Emission Line Profile Modeling Of Hot Stars

The launch of high-spectral-resolution x-ray telescopes (Chandra, XMM) has provided a host of new spectralline diagnostics for the astrophysics community. In this paper we discuss Doppler-broadened emission line profiles from highly supersonic outflows of massive stars. These outflows, or winds, are driven by radiation pressure and carry a tremendous amount of kinetic energy, which can be converted to x rays by shock-heating even a small fraction of the wind plasma. The unshocked, cold wind is a source of continuum opacity to the x rays generated in the shock-heated portion of the wind. Thus the emergent line profiles are affected by transport through a two-component, moving, optically thick medium. While complicated, the interactions among these physical effects can provide quantitative information about the spatial distribution and velocity of the x-ray-emitting and absorbing plasma in stellar winds. We present quantitative models of both a spherically symmetric wind and a wind with hot plasma confined in an equatorial disk by a dipole magnetic field

Synthesis and Assembly of Nonspherical Hollow Silica Colloids Under Confinement

Hard peanut-shaped colloids were synthesized and organized into a degenerate crystal (DC), a phase previously observed only in simulations. In this structure, particle lobes tile a triangular lattice while their orientations uniformly populate the three underlying crystalline directions