The CMS Electromagnetic Calorimeter at the LHC

The CMS detector at the LHC is in the final stages of preparation. The high resolution Electromagnetic Calorimeter, which consists of nearly 76000 lead tungstate crystals, will play a crucial role in the coming physics searches undertaken by CMS. The design, status, and initial performance of the calorimeter, in test beams and with cosmic rays, will be reviewed.Comment: ICHEP0

Last Settler’s Syndrome and Land Use Change in Southern Appalachia

In many rural areas land use patterns are shifting from agriculture or woodland to residential development. This is especially true in areas possessing significant natural amenities like the sweeping vistas, white-water rivers, and blue-ridged mountains of Southern Appalachia. As in-migration increases, decisions about land use can become heated as the “newcomers” square off with long-time residents. Understanding how various groups value both the land (as productive resource) and the landscape (as scenic amenity) can help address potential conflict when land use changes. Two independent projects in Watauga County, North Carolina, reflect attempts to understand perspectives on land use through an economic framework and to address land use change from an environmental perspective. Both projects reveal evidence of “last settler’s syndrome”—a tendency among individuals to place a high value on what initially attracted them to a specific place. Both also reveal situations of potential conflict when ideas about land use clash as well as situations for cooperation as various groups share values about land use. Key Words:

Determination of rolling element bearing condition via acoustic emission

Acoustic emission is an emerging technique for condition monitoring of rolling element bearings and potentially offers advantages for detection of incipient damage at an early stage of failure. Before such a technique can be applied with confidence for health monitoring, it is vital to understand the variation of acoustic emission generation with operating conditions in a healthy bearing. This paper investigates the effects of increased speed and load on the generation of acoustic emission within cylindrical roller bearings, and it was found that the root mean square signal level increased significantly with increasing speed whereas increasing load had a far weaker effect. The AERMS value for each experiment was compared with the trend of the Lambda value. The bearing was operating under full film lubrication regime, so it was determined that increases in AERMS were not caused by asperity contact. By consideration of trends in frequency energy amplitude, it was determined that excitation of the bearings resonant frequencies were responsible for an increase of energy in the frequency range of 20–60 kHz. The excitation energy at 330 kHz (the acoustic emission sensor’s resonant frequency) increased with load, indicating a link between high-frequency emission and stress at the contact zone. Following characterisation of the bearing under normal operating conditions, an accelerated life test was conducted in order to induce fatigue failure. The frequency response demonstrated that throughout a period of constant wear, the energy amplitude at the bearings resonant frequency increased with time. As the bearing failure became more significant, the energy of the high-frequency components above 100 kHz was spread over a broader frequency range as multiple transient bursts of energy were released simultaneously by fatigue failure of the raceways. This paper demonstrates the potential of acoustic emission to provide an insight into the bearing’s behaviour under normal operation and provide early indication of bearing failure

Soup to tree: the phylogeny of beetles inferred by mitochondrial metagenomics of a Bornean rainforest sample

In spite of the growth of molecular ecology, systematics and next-generation sequencing, the discovery and analysis of diversity is not currently integrated with building the tree-of-life. Tropical arthropod ecologists are well placed to accelerate this process if all specimens obtained via masstrapping, many of which will be new species, could be incorporated routinely in phylogeny reconstruction. Here we test a shotgun sequencing approach, whereby mitochondrial genomes are assembled from complex ecological mixtures via mitochondrial metagenomics, and demonstrate how the approach overcomes many of the taxonomic impediments to the study of biodiversity. DNA from ~500 beetle specimens, originating from a single rainforest canopy fogging sample from Borneo, was pooled and shotgun sequenced, followed by de novo assembly of complete and partial mitogenomes for 175 species. The phylogenetic tree obtained from this local sample was highly similar to that from existing mitogenomes selected for global coverage of major lineages of Coleoptera. When all sequences were combined, only minor topological changes are induced against this reference set, indicating an increasingly stable estimate of coleopteran phylogeny, whilst the ecological sample expands the tip-level representation of several lineages. Robust trees generated from ecological samples now enable an evolutionary framework for ecology. Meanwhile, the inclusion of uncharacterized samples in the tree-of-life rapidly expands taxon and biogeographic representation of lineages without morphological identification. Mitogenomes from shotgun sequencing of unsorted environmental samples and their associated metadata, placed robustly into the phylogenetic tree, constitute novel DNA ‘superbarcodes’ for testing hypotheses regarding global patterns of diversity

Suitability of litter amendments for the Australian chicken meat industry

This project focused on litter amendment products, which are used overseas during the rearing of meat chickens. Litter amendments are primarily used to manage ammonia volatilisation, especially when litter is reused, but also provide antimicrobial and environmental benefits, and increase the nutrient value of spent litter. This report summarises the outcomes of consultation with representatives and stakeholders of the Australian chicken meat industry, and summarises key findings from a literature review on litter amendments

Blockade of T-cell activation by dithiocarbamates involves novel mechanisms of inhibition of nuclear factor of activated T cells.

Dithiocarbamates (DTCs) have recently been reported as powerful inhibitors of NF-kappaB activation in a number of cell types. Given the role of this transcription factor in the regulation of gene expression in the inflammatory response, NF-kappaB inhibitors have been suggested as potential therapeutic drugs for inflammatory diseases. We show here that DTCs inhibited both interleukin 2 (IL-2) synthesis and membrane expression of antigens which are induced during T-cell activation. This inhibition, which occurred with a parallel activation of c-Jun transactivating functions and expression, was reflected by transfection experiments at the IL-2 promoter level, and involved not only the inhibition of NF-kappaB-driven reporter activation but also that of nuclear factor of activated T cells (NFAT). Accordingly, electrophoretic mobility shift assays (EMSAs) indicated that pyrrolidine DTC (PDTC) prevented NF-kappaB, and NFAT DNA-binding activity in T cells stimulated with either phorbol myristate acetate plus ionophore or antibodies against the CD3-T-cell receptor complex and simultaneously activated the binding of AP-1. Furthermore, PDTC differentially targeted both NFATp and NFATc family members, inhibiting the transactivation functions of NFATp and mRNA induction of NFATc. Strikingly, Western blotting and immunocytochemical experiments indicated that PDTC promoted a transient and rapid shuttling of NFATp and NFATc, leading to their accelerated export from the nucleus of activated T cells. We propose that the activation of an NFAT kinase by PDTC could be responsible for the rapid shuttling of the NFAT, therefore transiently converting the sustained transactivation of this transcription factor that occurs during lymphocyte activation, and show that c-Jun NH2-terminal kinase (JNK) can act by directly phosphorylating NFATp. In addition, the combined inhibitory effects on NFAT and NF-KB support a potential use of DTCs as immunosuppressants