Effect of cycloheximide and actinomycin D on germinating conidia

Effect of cycloheximide and actinomycin D on germinating conidi

Conditional quantum-state transformation at a beam splitter

Using conditional measurement on a beam splitter, we study the transformation of the quantum state of the signal mode within the concept of two-port non-unitary transformation. Allowing for arbitrary quantum states of both the input reference mode and the output reference mode on which the measurement is performed, we show that the non-unitary transformation operator can be given as an $s$-ordered operator product, where the value of $s$ is entirely determined by the absolute value of the beam splitter reflectance (or transmittance). The formalism generalizes previously obtained results that can be recovered by simple specification of the non-unitary transformation operator. As an application, we consider the generation of Schr\"odinger-cat-like states. An extension to mixed states and imperfect detection is outlined.Comment: 7 Postscript figures, using Late

Macroscopically distinct quantum superposition states as a bosonic code for amplitude damping

We show how macroscopically distinct quantum superposition states (Schroedinger cat states) may be used as logical qubit encodings for the correction of spontaneous emission errors. Spontaneous emission causes a bit flip error which is easily corrected by a standard error correction circuit. The method works arbitrarily well as the distance between the amplitudes of the superposed coherent states increases.Comment: 4 pages, 2 postscript figures, LaTeX2e, RevTeX, minor changes, 1 reference adde

Synthesis and characterization of entangled mesoscopic superpositions for a trapped electron

We propose a scheme for the generation and reconstruction of entangled states between the internal and external (motional) degrees of freedom of a trapped electron. Such states also exhibit quantum coherence at a mesoscopic level.Comment: 4 pages, 1 figure, RevTeX (twocolumn

Kondo Temperature for the Two-Channel Kondo Models of Tunneling Centers

The possibility for a two-channel Kondo ($2CK$) non Fermi liquid state to appear in a metal as a result of the interaction between electrons and movable structural defects is revisited. As usual, the defect is modeled by a heavy particle moving in an almost symmetric double-well potential (DWP). Taking into account only the two lowest states in DWP is known to lead to a Kondo-like Hamiltonian with rather low Kondo temperature, $T_K$. We prove that, in contrast to previous believes, the contribution of higher excited states in DWP does not enhance $T_K$. On the contrary, $T_K$ is reduced by three orders of magnitude as compared with the two-level model: the prefactor in $T_K$ is determined by the spacing between the second and the third levels in DWP rather than by the electron Fermi energy. Moreover, $T_K$, turns out to be parametrically smaller than the splitting between the two lowest levels. Therefore, there is no microscopic model of movable defects which may justify non-Fermi liquid $2CK$ phenomenology.Comment: 5 pages, 4 .eps figure

Zonal pressure gradient along the equatorial Atlantic

For three consecutive periods during the summer of 1974, ships of many nations made observations along the Atlantic equator as part of the GATE program [GARP (Global Atmospheric Research Program) Atlantic Tropical Experiment]. Combining these observations, it is found that the zonal pressure gradient over the central Atlantic at the surface and at 50 dbar, relative to 500 dbar, increased from 3.2 to 7.3 and 2.2 to 5.3 × 10-5 dynes/g respectively between June/July and August and then held close to the high values in September...

Survey of the quality of experimental design, statistical analysis and reporting of research using animals

For scientific, ethical and economic reasons, experiments involving animals should be appropriately designed, correctly analysed and transparently reported. This increases the scientific validity of the results, and maximises the knowledge gained from each experiment. A minimum amount of relevant information must be included in scientific publications to ensure that the methods and results of a study can be reviewed, analysed and repeated. Omitting essential information can raise scientific and ethical concerns. We report the findings of a systematic survey of reporting, experimental design and statistical analysis in published biomedical research using laboratory animals. Medline and EMBASE were searched for studies reporting research on live rats, mice and non-human primates carried out in UK and US publicly funded research establishments. Detailed information was collected from 271 publications, about the objective or hypothesis of the study, the number, sex, age and/or weight of animals used, and experimental and statistical methods. Only 59% of the studies stated the hypothesis or objective of the study and the number and characteristics of the animals used. Appropriate and efficient experimental design is a critical component of high-quality science. Most of the papers surveyed did not use randomisation (87%) or blinding (86%), to reduce bias in animal selection and outcome assessment. Only 70% of the publications that used statistical methods described their methods and presented the results with a measure of error or variability. This survey has identified a number of issues that need to be addressed in order to improve experimental design and reporting in publications describing research using animals. Scientific publication is a powerful and important source of information; the authors of scientific publications therefore have a responsibility to describe their methods and results comprehensively, accurately and transparently, and peer reviewers and journal editors share the responsibility to ensure that published studies fulfil these criteria

Roles for Plant Mitochondrial Alternative Oxidase Under Normoxia, Hypoxia, and Reoxygenation Conditions

Alternative oxidase (AOX) is a non-energy conserving terminal oxidase in the plant mitochondrial electron transport chain (ETC) that has a lower affinity for oxygen than does cytochrome (cyt) oxidase. To investigate the role(s) of AOX under different oxygen conditions, wild-type (WT) Nicotiana tabacum plants were compared with AOX knockdown and overexpression plants under normoxia, hypoxia (near-anoxia), and during a reoxygenation period following hypoxia. Paradoxically, under all the conditions tested, the AOX amount across plant lines correlated positively with leaf energy status (ATP/ADP ratio). Under normoxia, AOX was important to maintain respiratory carbon flow, to prevent the mitochondrial generation of superoxide and nitric oxide (NO), to control lipid peroxidation and protein S-nitrosylation, and possibly to reduce the inhibition of cyt oxidase by NO. Under hypoxia, AOX was again important in preventing superoxide generation and lipid peroxidation, but now contributed positively to NO amount. This may indicate an ability of AOX to generate NO under hypoxia, similar to the nitrite reductase activity of cyt oxidase under hypoxia. Alternatively, it may indicate that AOX activity simply reduces the amount of superoxide scavenging of NO, by reducing the availability of superoxide. The amount of inactivation of mitochondrial aconitase during hypoxia was also dependent upon AOX amount, perhaps through its effects on NO amount, and this influenced carbon flow under hypoxia. Finally, AOX was particularly important in preventing nitro-oxidative stress during the reoxygenation period, thereby contributing positively to the recovery of energy status following hypoxia. Overall, the results suggest that AOX plays a beneficial role in low oxygen metabolism, despite its lower affinity for oxygen than cytochrome oxidase

Within study comparisons and risk of bias in international development: Systematic review and critical appraisal

Background Many systematic reviews incorporate nonrandomised studies of effects, sometimes called quasi‐experiments or natural experiments. However, the extent to which nonrandomised studies produce unbiased effect estimates is unclear in expectation or in practice. The usual way that systematic reviews quantify bias is through “risk of bias assessment” and indirect comparison of findings across studies using meta‐analysis. A more direct, practical way to quantify the bias in nonrandomised studies is through “internal replication research”, which compares the findings from nonrandomised studies with estimates from a benchmark randomised controlled trial conducted in the same population. Despite the existence of many risks of bias tools, none are conceptualised to assess comprehensively nonrandomised approaches with selection on unobservables, such as regression discontinuity designs (RDDs). The few that are conceptualised with these studies in mind do not draw on the extensive literature on internal replications (within‐study comparisons) of randomised trials. Objectives Our research objectives were as follows: Objective 1: to undertake a systematic review of nonrandomised internal study replications of international development interventions. Objective 2: to develop a risk of bias tool for RDDs, an increasingly common method used in social and economic programme evaluation. Methods We used the following methods to achieve our objectives. Objective 1: we searched systematically for nonrandomised internal study replications of benchmark randomised experiments of social and economic interventions in low‐ and middle‐income countries (L&MICs). We assessed the risk of bias in benchmark randomised experiments and synthesised evidence on the relative bias effect sizes produced by benchmark and nonrandomised comparison arms. Objective 2: We used document review and expert consultation to develop further a risk of bias tool for quasi‐experimental studies of interventions (ROBINS‐I) for RDDs. Results Objective 1: we located 10 nonrandomised internal study replications of randomised trials in L&MICs, six of which are of RDDs and the remaining use a combination of statistical matching and regression techniques. We found that benchmark experiments used in internal replications in international development are in the main well‐conducted but have “some concerns” about threats to validity, usually arising due to the methods of outcomes data collection. Most internal replication studies report on a range of different specifications for both the benchmark estimate and the nonrandomised replication estimate. We extracted and standardised 604 bias coefficient effect sizes from these studies, and present average results narratively. Objective 2: RDDs are characterised by prospective assignment of participants based on a threshold variable. Our review of the literature indicated there are two main types of RDD. The most common type of RDD is designed retrospectively in which the researcher identifies post‐hoc the relationship between outcomes and a threshold variable which determines assignment to intervention at pretest. These designs usually draw on routine data collection such as administrative records or household surveys. The other, less common, type is a prospective design where the researcher is also involved in allocating participants to treatment groups from the outset. We developed a risk of bias tool for RDDs. Conclusions Internal study replications provide the grounds on which bias assessment tools can be evidenced. We conclude that existing risk of bias tools needs to be further developed for use by Campbell collaboration authors, and there is a wide range of risk of bias tools and internal study replications to draw on in better designing these tools. We have suggested the development of a promising approach for RDD. Further work is needed on common methodologies in programme evaluation, for example on statistical matching approaches. We also highlight that broader efforts to identify all existing internal replication studies should consider more specialised systematic search strategies within particular literatures; so as to overcome a lack of systematic indexing of this evidence