Differences in channel and hillslope geometry record a migrating uplift wave at the Mendocino Triple Junction

Tectonic plate motion, and the resulting change in land surface elevation, has been shown to have a fundamental impact on landscape morphology. Changes to uplift rates can drive a response in fluvial channels, which then drives changes to hillslopes. Because hillslopes respond on different time scales than fluvial channels, investigating the geometry of channels and hillslopes in concert provides novel opportunities to examine how uplift rates may have changed through time. Here we perform coupled topographic analysis of channel and hillslope geometry across a series of catchments at the Mendocino triple junction (MTJ) in northern California, USA. These catchments are characterized by an order-of-magnitude difference in uplift rate from north to south. We find that dimensionless hillslope relief closely matches the uplift signal across the area and is positively correlated with channel steepness. Furthermore, the range of uncertainty in hillslope relief is lower than that of channel steepness, suggesting that it may be a more reliable recorder of uplift in the MTJ region. We find that hilltop curvature lags behind relief in its response to uplift, which in turn lags behind channel response. These combined metrics show the northward migration of the MTJ and the corresponding uplift field from topographic data alone

Identifying and Characterizing a Functional HIV-1 Reverse Transcriptase-binding Site on Integrase*

Integrase (IN) from human immunodeficiency virus, type 1 (HIV-1) exerts pleiotropic effects in the viral replication cycle. Besides integration, IN mutations can impact nuclear import, viral maturation, and reverse transcription. IN and reverse transcriptase (RT) interact in vitro, and the IN C-terminal domain (CTD) is both necessary and sufficient for binding RT. We used nuclear magnetic resonance spectroscopy to identify a putative RT-binding surface on the IN CTD, and surface plasmon resonance to obtain kinetic parameters and the binding affinity for the IN-RT interaction. An IN K258A substitution that disrupts reverse transcription in infected cells is located at the putative RT-binding surface, and we found that this substitution substantially weakens IN CTD-RT interactions. We also identified two additional IN amino acid substitutions located at the putative RT-binding surface (W243E and V250E) that significantly impair viral replication in tissue culture. These results strengthen the notion that IN-RT interactions are biologically relevant during HIV-1 replication and also provide insights into this interaction at the molecular level

The damping of a quartz tuning fork in superfluid He-3-B at low temperatures

We have measured the damping on a quartz tuning fork in the B-phase of superfluid He-3 at low temperatures, below 0.3T (c). We present extensive measurements of the velocity dependence and temperature dependence of the damping force. At the lowest temperatures the damping is dominated by intrinsic dissipation at low velocities. Above some critical velocity an extra temperature independent damping mechanism quickly dominates. At higher temperatures there is additional damping from thermal quasiparticle excitations. The thermal damping mechanism is found to be the same as that for a vibrating wire resonator; Andreev scattering of thermal quasiparticles from the superfluid back-flow leads to a very large damping force. At low velocities the thermal damping force varies linearly with velocity, but tends towards a constant at higher velocities. The thermal damping fits very well to a simple model developed for vibrating wire resonators. This is somewhat surprising, since the quasiparticle trajectories through the superfluid flow around the fork prongs are more complicated due to the relatively high frequency of motion. We also discuss the damping mechanism above the critical velocity and compare the behaviour with other vibrating structures in superfluid He-3-B and in superfluid He-4 at low temperatures. In superfluid He-4 the high velocity response is usually dominated by vortex production (quantum turbulence), however in superfluid He-3 the response may either be dominated by pair-breaking or by vortex production. In both cases the critical velocity in superfluid He-3-B is much smaller and the high velocity drag coefficient is much larger, compared to equivalent measurements in superfluid He-4