Quantifying the Differences in Binding Affinity of Reduced Glutathione for Glutathione S-Transferase at pH 6.5 and 8.5 Using Isothermal Titration Calorimetry

The binding affinity between an enzyme and its substrate is often dependent on the pH of the local environment. Knowing the pH at which reduced glutathione (GSH) binds with the highest affinity to the enzyme glutathione S-transferase (GST) is useful for determining the optimal pH for purification of GST-fusion proteins during GST-affinity chromatography. In this study, GST of the species Schistosoma japonicum was purified, quantified, and utilized to study its binding interaction with GSH at pH 6.5 and 8.5 via isothermal titration calorimetry (ITC). After protein expression, extraction, and purification, the GST concentration was quantified using QubitTM fluorometry. Thermodynamic properties and a dissociation constant (KD) for each experiment were obtained utilizing the MicroCal PEAQ-ITC Analysis Software for the binding of GSH to GST at pH 6.5 and 8.5. Statistical analysis of the technical replicate data was performed to obtain an average and standard deviation of the KD at each pH point. The results indicate a statistically significant difference (

The ubiquitous 1100 charge ordering in organic charge-transfer solids

Charge and spin-orderings in the 1/4-filled organic CT solids are of strong interest, especially in view of their possible relations to organic superconductivity. We show that the charge order (CO) in both 1D and 2D CT solids is of the ...1100... type, in contradiction to mean field prediction of >...1010... CO. We present detailed computations for metal-insulator and magnetic insulator-insulator transitions in the theta-ET materials. Complete agreement with experiments in several theta systems is found. Similar comparisons between theory and experiments in TCNQ, TMTTF, TMTSF, and ET materials prove the ubiquity of this phenomenon.Comment: 3 pages, 4 eps figures; ICSM 200

GC3 of genes can be used as a proxy for isochore base composition: A reply to Elhaik et al.

In an article published in these pages, Elhaik et al. (Elhaik E, Landan G, Graur D. 2009. Can GC content at third-codon positions be used as a proxy for isochore composition? Mol Biol Evol. 26:1829-1833) asked if GC3, the GC level of the third-codon positions in protein-coding genes, can be used as a 'proxy' to estimate the GC level of the surrounding isochore. We use available data to directly answer this simple question in the affirmative and show how the use of indirect methods can lead to apparently conflicting conclusions. The answer reasserts that in human and other vertebrates, genes have a strong tendency to reside in compositionally corresponding isochores, which has far-reaching implications for genome structure and evolution. © 2010 The Author

Variation in specificity of soil-borne pathogens from a plant's native range versus its nonnative range

Contains fulltext : 93607.pdf (publisher's version ) (Open Access

Rapid characterization of the ultraviolet induced fiber Bragg grating complex coupling coefficient as a function of irradiance and exposure time

We report the application of optical frequency domain reflectometry and a discrete-layer-peeling inverse scattering algorithm to the spatial characterization of the UV induced complex coupling coefficient during fiber Bragg grating growth. The fiber grating is rapidly characterized using this technique to give irradiance dependent growth as a function of exposure time, thereby providing the complete characterization of the coupling coefficient in the form of a "growth surface," which is related to the fiber's photosensitivity. We compare measurements of fiber Bragg grating growth in SMF-28 when exposed to continuous wave 244 nm irradiation from 0 to 90 W cm(-2) for exposure times up to 3230 s with a selection of other fibers including high germanium concentration fiber and erbium doped fiber. (c) 2007 Optical Society of America

The Use of Weather Data and Evapotranspiration Requirements to Estimate the Marginal Value Product of Irrigation and the Profit Maximizing Irrigation Level for Corn in the Texas Panhandle

The declining availability of irrigation water from the Ogallala aquifer combined with increasing energy costs make irrigation strategies much more critical. Maximizing yield reduces profit by between $22 and$158 per acre depending on the combination of corn and natural gas prices.corn, irrigation efficiency, water response function, evapotranspiration, Crop Production/Industries, Land Economics/Use, Research and Development/Tech Change/Emerging Technologies,

Herding Cats: The Role of Coordination Mechanisms in a Federated IS Governance Structure with Highly Autonomous Sub-Units

Lateral mechanisms are used to promote coordination across independent, highly autonomous IS subunits in large, complex organizations. Effective coordination offers significant opportunities to gain economies of scale and scope while maintaining the benefits of sub-unit independence. The objective of this study is to develop a deeper understanding of the factors and contingencies that determine the choice and effectiveness of various coordination mechanisms in a federated IS governance context. This paper presents a review of the theoretical foundations of lateral relationships, the conceptual model of the study, a detailed review of the role of coordination mechanisms, and ends with the study’s research model and specific research propositions. This paper describes an in-depth, longitudinal case study that is currently in process

Using RF-DNA Fingerprints to Discriminate ZigBee Devices in an Operational Environment

This research was performed to expand AFIT\u27s Radio Frequency Distinct Native Attribute (RF-DNA) fingerprinting process to support IEEE 802.15.4 ZigBee communication network applications. Current ZigBee bit-level security measures include use of network keys and MAC lists which can be subverted through interception and spoofing using open-source hacking tools. This work addresses device discrimination using Physical (PHY) waveform alternatives to augment existing bit-level security mechanisms. ZigBee network vulnerability to outsider threats was assessed using Receiver Operating Characteristic (ROC) curves to characterize both Authorized Device ID Verification performance (granting network access to authorized users presenting true bit-level credentials) and Rogue Device Rejection performance (denying network access to unauthorized rogue devices presenting false bit-level credentials). Radio Frequency Distinct Native Attribute (RF-DNA) features are extracted from time-domain waveform responses of 2.4 GHz CC2420 ZigBee transceivers to enable humanlike device discrimination. The fingerprints were constructed using a hybrid pool of emissions collected under a range of conditions, including anechoic chamber and an indoor office environment where dynamic multi-path and signal degradation factors were present. The RF-DNA fingerprints were input to a Multiple Discriminant Analysis, Maximum Likelihood (MDA/ML) discrimination process and a 1 vs. many Looks most like? classification assessment made. The hybrid MDA model was also used for 1 vs. 1 Looks how much like? verification assessment. ZigBee Device Classification performance was assessed using both full and reduced dimensional fingerprint sets. Reduced dimensional subsets were selected using Dimensional Reduction Analysis (DRA) by rank ordering 1) pre-classification KS-Test p-values and 2) post-classification GRLVQI feature relevance values. Assessment of Zigbee device ID verification capability