EMA space: a collaborative workspace as collaborative urban ecosystem generator?

International audienceFollowing the industrial background, the necessity of the innovation is found at the level of the urban systems. The innovations are not only researched at the level of the results of the urban projects but also in the processes which see them born and develop. In addition, both the societal evolutions, described by the social and human sciences, and the results of multidisciplinary researches wonder about the involvement of the citizens / users in the process of output of the city. The emergence, for few years, of the Living Labs probably gives new opportunities to lead a reflection on the involvement of the citizens in the urban projects. From then on, it consists in mixing the questionings held by the user-driven open innovation and the stakes of "democratization" of the urban projects. It is in particular what suggests the Lorraine (NIT) Smart Cities Living Lab

Quand l'Equateur est envisagé comme une frontière : étude d'une stratégie d'écriture du romancier équatorien Javier Vásconez

International audienc

From urban concept to urban engineering: The contribution of distributed collaborative design to the management of urban projects

International audienceThe complexity of urban systems is an increasingly common topic in academic literature. Following in the footsteps of the industrial sector, which has understood this issue for many years, urban engineering must also tackle the challenges created by complex systems. Industrial engineering has provided a number of responses to this challenge, including design technologies, which are notably collaborative. It seems possible, at least in theory, to transfer a number of best practice methods and adapt these to the conceptualization of urban development projects (in the initial phase) in order to encourage their global management (in terms of strategic decision making) and their social acceptability. The challenge is then to formulate new methodological models, as well as to create an environment dedicated to their application

Development of liquid chromatography methods coupled to mass spectrometry for the analysis of substances with a wide variety of polarity in meconium.

International audienceMeconium is the first fecal excretion of newborns. This complex accumulative matrix allows assessing the exposure of the fetus to xenobiotics during the last 6 months of pregnancy. To determine the eventual effect of fetal exposure to micropollutants in this matrix, robust and sensitive analytical methods must be developed. This article describes the method development of liquid chromatography methods coupled to triple quadrupole mass spectrometry for relevant pollutants. The 28 selected target compounds had different physico-chemical properties from very polar (glyphosate) to non-polar molecules (pyrethroids). Tests were performed with three different types of columns: reversed phase, ion exchange and HILIC. As a unique method could not be determined for the simultaneous analysis of all compounds, three columns were selected and suitable chromatographic methods were optimized. Similar results were noticed for the separation of the target compounds dissolved in either meconium extract or solvent for reversed phase and ion exchange columns. However, for HILIC, the matrix had a significant influence on the peak shape and robustness of the method. Finally, the analytical methods were applied to “real” meconium samples

Compensation des exportations minérales et remédiations aux dégradations des sols. Compte rendu de l’atelier 3

Les forêts françaises font face aujourd’hui à des pressions extérieures croissantes. Ces contraintes sont d’ordre nutritionnel ou sylvicole ou climatique et risquent d’impacter la durabilité des écosystèmes forestiers. Le sol peut être impacté, entraînant une dégradation de ses composantes chimique ou physique ou biologique. L’objectif de l’atelier était d’apporter des éléments de connaissance et de débat autour des différents types de dégradation, de remédiation et de restauration des sols. Cet atelier a également permis d’échanger autour de différentes approches permettant de soutenir la productivité forestière, par l’utilisation d’intrants de différentes natures (amendements/cendres, boues de stations d’épuration…) ou par l’utilisation des friches industrielles pour produire de la biomasse

Aberrant Splicing Is the Pathogenicity Mechanism of the p.Glu314Lys Variant in CYP11A1 Gene

Context: The cholesterol side chain cleavage enzyme (CYP11A1) catalyzes the conversion of cholesterol to pregnenolone, the first rate-limiting step of steroidogenesis. CYP11A1 mutations are associated with primary adrenal insufficiency (PAI) as well as disorders of sex development (DSD) in 46,XY patients.Objective: To define the pathogenicity mechanism for the p.Glu314Lys variant, previously reported, and found in four additional patients with CYP11A1 deficiency.Subjects and Methods: DNA of four patients presenting with delayed PAI and/or 46,XY DSD were studied by Sanger or Massively Parallel sequencing. Three CYP11A1 mutations were characterized in vitro and in silico, and one by mRNA analysis on testicular tissue.Results: All patients were compound heterozygous for the previously described p.Glu314Lys variant. In silico studies predicted this mutation as benign with no effect on splicing but mRNA analysis found that it led to incomplete exon 5 skipping. This mechanism was confirmed by minigene experiment. The protein carrying this mutation without exon skipping should conserve almost normal activity, according to in vitro studies. Two other mutations found in trans, the p.Arg120Gln and p.Arg465Trp, had similar activity compared to negative control, consistent with the in silico studies.Conclusions: We provide biological proof that the p. Glu314Lys variant is pathogenic due to its impact on splicing and seems responsible for the moderate phenotype of the four patients reported herein. The present study highlights the importance of considering the potential effect of a missense variant on splicing when it is not predicted to be disease causing

Le monopole de la Casa de la Cultura Ecuatoriana : bilan critique de l'adhésion des intellectuels à l'entreprise culturelle proposée par Benjamin Carrión en 1944

Le processus d'institutionnalisation de la culture en Equateur, matérialisé par la Casa de la Cultura Ecuatoriana, a engendré un phénomène qui a bel et bien contribué au développement culturel de la nation, ainsi que l'avait rêvé et conçu Benjamín Carrión dans les textes qui ont servi de fondement théorique à cet organisme : ce phénomène est la situation de monopole de l'institution, tout au long d'une vingtaine d'année. Les premières activités développées par l'institution manifestent claire..

Polémique équatorienne autour des tableaux Los Projetas

Morel Anne-Claudine. Polémique équatorienne autour des tableaux Los Projetas. In: América : Cahiers du CRICCAL, n°21, 1998. Polémiques et manifestes aux XIXe et XXe siècles en Amérique latine. pp. 245-254

Les Maisons de la Culture française (1934) et la Casa de la Cul- tura Ecuatoriana (1944) : Filiation

Morel Anne-Claudine. Les Maisons de la Culture française (1934) et la Casa de la Cul- tura Ecuatoriana (1944) : Filiation. In: América : Cahiers du CRICCAL, n°19, 1997. Les filiations. Idées et cultures contemporaines en Amérique Latine. pp. 141-151