Who plays with whom: farrowing environment influences isolation of foster piglets in play

Cross fostering piglets is a common management practise in the pig industry to manage large and heterogeneous litters, whereby piglets are moved from their biological litter to be reared by another sow. At present research has focused on immediate survival consequences and time of cross fostering, with little attention given to positive aspects of welfare such as social affiliations and the potential for positive interactions for these piglets such as play behaviour. The focus of our study was purely observational to record behaviour of piglets reared in either impoverished (farrowing crates) or enriched neonatal environments (PigSAFE pens) where fostering was practised as part of normal husbandry routines to promote piglet survival. We employed social network analysis to understand more about the behaviour of foster piglets in these environments and their acceptance into their new litter. In line with previous work, piglets exposed to enriched neonatal farrowing pens demonstrated more play behaviour than piglets reared in farrowing crates. We showed that pen piglets received and initiated more play invitations (higher degree centrality) than piglets in crates. We also found effects of cross fostering irrespective of neonatal environment. Non-foster piglets received and initiated more play behaviours (higher degree centrality) 2–3 weeks post-farrowing compared to piglets fostered into the litter and as such, fostered piglets remained isolated from play for the first few weeks of life. However, our data suggests this may be mitigated by neonatal environment; foster piglets reared in pens were better connected (betweenness centrality) within their foster litter than those fostered in crates. Our findings highlight the importance of the neonatal environment and its potential influence on the isolation of cross-fostered piglets and suggest that rearing in enriched neonatal environments may help mitigate against social isolation in early life of cross-fostered piglets, having obvious immediate, and long-term consequences for piglet welfare and behaviour. We also highlight the importance and application of methodologies such as social network analysis, for gaining more insight and understanding about the sociality of animal behaviour and its potential for measuring indicators of positive welfare, thus highlighting its application for veterinary science and animal behaviour and welfare research

Using consummatory behaviour to measure the affective state and welfare of laboratory mice

PhD ThesisMice are the most widely used model in biomedical research, making it important to know how life in the laboratory impacts on their welfare. Whilst it is difficult to evaluate how a mouse might ‘feel’ because of their inability to self-report, behavioural and physiological measures can give insights into their current welfare state. One approach is to measure their ‘affective state’ through how they respond to reward. Humans with depression often report a lack of enjoyment from reward (known as anhedonia), which can also be measured in laboratory rodents by measuring sucrose consumption: the less they consume, the more anhedonic they are. However, consumption is confounded by other factors, particularly motivational state. Therefore, an alternative method assesses the microstructure of the animals’ licking patterns, which may better reflect an animal’s hedonic response towards reward, i.e. how much it ‘likes’ it. The aim of my thesis was to determine how stress influences the hedonic responses of laboratory mice, and determine whether assessing changes in consumption or licking microstructure could be used to infer a mouse’s affective state, in order to make evidence-based improvements to their welfare. Experiments using standard depressogenic methods (i.e. chronic mild stress and chronic corticosterone administration) were ineffective at altering affective state, and sucrose consumption and licking microstructure were unchanged. However, I found that current methods used to handle laboratory mice were sufficient in inducing changes in the animal’s affective state. I found that the standard practice of handling mice by their tails causes alterations in reward perception, revealing a depressivelike state. These experiments provide more support for refinements to be made with regards to the existing handling practices of laboratory mice. I discuss my findings in relation to implications for animal welfare and scientific data collection across a number of in-vivo modelsBBSR

The perspectives of UK personnel towards current killing practices for laboratory rodents

Data supporting manuscript entitled 'The perspectives of UK personnel towards current killing practices for laboratory rodents.

A decade on where is the UK poultry industry for emergency on-farm killing?

Millions of poultry are farmed intensively every year across the United Kingdom (UK) to produce both meat and eggs. There are inevitable situations that require birds to be emergency killed on farm to alleviate pain and suffering. In Europe and the UK, emergency methods are regulated by the European Council Regulation (EC) No. 1099/2009 and The Welfare of Animals at the Time of Killing Regulations (England 2015; Scotland 2012; Wales and Northern Ireland 2014). Cervical dislocation has been reported to be the most widely used method prior to these legislative changes which took place from 1 January 2013. Based on limited scientific evidence and concern for bird welfare, these legislative changes incorporated restrictions based on bird weight for both manual (≤3 kg) and mechanical (≤5 kg) cervical dislocation, and introduced an upper limit in the number of applications for manual cervical dislocation (up to 70 birds per person per day). Furthermore, it removed methods which showed evidence of crushing injury to the neck. However, since legal reform new scientific evidence surrounding the welfare consequences of cervical dislocation and the development of novel methods for killing poultry in small numbers on farm have become available. Whether the UK poultry industry have adopted these novel methods, and whether legislative reform resulted in a change in the use of cervical dislocation in the UK remains unknown. Responses from 215 respondents working across the UK poultry industry were obtained. Despite legal reform, manual cervical dislocation remains the most prevalent method used across the UK for killing poultry on farm (used by 100% of farms) and remains the preferred method amongst respondents (81.9%). The use of alternative methods such as Livetec Nex® and captive bolt guns were available to less than half of individuals and were not frequently employed for broilers and laying hens. Our data suggests there is a lack of a clear alternative to manual cervical dislocation for individuals working with larger species and a lack of gold standard methodology. This risks bird welfare at killing and contributes to inconsistency across the industry. We suggest providing stakeholders with practical alternatives prior to imposing legislative changes and effective knowledge transfer between the scientific community and stakeholders to promote positive change and protect bird welfare

A review of methods used to kill laboratory rodents: issues and opportunities

Rodents are the most widely used species for scientific purposes. A critical pre-requisite of their use, based on utilitarian ethical reasoning, is the provision of a humane death when necessary for scientific or welfare grounds. Focussing on the welfare challenges presented by current methods, we critically evaluate the literature, consider emerging methodologies that may have potential for refinement and highlight knowledge gaps for future research. The evidence supports the conclusion that scientists and laboratory personnel should seek to avoid killing laboratory rodents by exposing them to carbon dioxide (CO2), unless exploiting its high-throughput advantage. We suggest that stakeholders and policymakers should advocate for the removal of CO2 from existing guidelines, instead making its use conditionally acceptable with justification for additional rationale for its application. With regards to physical methods such as cervical dislocation, decapitation and concussion, major welfare concerns are based on potential inaccuracy in application and their susceptibility to high failure rates. There is a need for independent quality-controlled training programmes to facilitate optimal success rates and the development of specialist tools to improve outcomes and reliability. Furthermore, we highlight questions surrounding the inconsistent inclusion criteria and acceptability of physical methods in international regulation and/or guidance, demonstrating a lack of cohesion across countries and lack of a comprehensive ‘gold standard’ methodology. We encourage better review of new data and championing of open access scientific resources to advocate for best practice and enable significant changes to policy and legislation to improve the welfare of laboratory rodents at killing

Determining candidate hypobaric hypoxia profiles for humane killing of laboratory mice

Millions of mice are used annually in scientific research and must be humanely killed. Despite significant welfare concerns, carbon dioxide exposure remains the most common killing method, primarily because there is no practical and humane alternative. We explored whether hypobaric hypoxia via gradual decompression could induce a non-recovery state in anesthetized male C57BL/6 and Balb/c laboratory mice. We aimed to determine if this was possible in a feasible timescale with minimal pathological consequences, as a proof-of-principle step. Systematic evaluation of two decompression rates (75, 150 ms(−1)) and three profile shapes (accelerated, linear, gradual) in a factorial design revealed that hypobaric hypoxia effectively induced a non-recovery state in anesthetized laboratory mice, irrespective of decompression rate and shape. Mice took longer to reach a non-recovery state with the 75 ms(−1) decompression rate (75 ms(−1): 257 ± 8.96 vs. 150 ms(−1): 214 ± 7.26 s), with longer latencies in gradual and linear shaped profiles. Accelerated shaped profiles were least susceptible to meaningful refinement via rate. The only pathological changes of concern were moderate middle ear congestion and hemorrhage. These findings suggest that hypobaric hypoxia has potential, and subsequent work will evaluate the welfare consequences of gradual decompression in conscious mice, to identify decompression profiles that minimize welfare harms associated with ear barotrauma

Evaluating pain and analgesia effectiveness following routine castration in rabbits using behaviour and facial expressions

Prevention of pain in rabbits is a priority for both welfare and validity of scientific data. We aimed to determine if the rabbit grimace scale (RbtGS) could be used as a viable, rapid assessment tool in two breeds of rabbit, Dutch belted (DB) and New Zealand white (NZW), following orchidectomy, as an adjunct to behavioral analysis. All animals received analgesia. Rabbits were filmed and their behavior was recorded at multiple time points pre- and post-orchidectomy. Observers then scored specific pain associated behaviors for analysis. Time matched footage was also scored using the rabbit grimace scale (RbtGS). Following surgery, rabbits showed significant increases in the duration spent displaying key pain associated behaviors at 1 and 5 h post-surgery. DB rabbits that received low dose meloxicam (0.2 mg/kg) showed significantly more pain behaviors at 1 and 5 h post-surgery compared to those administered a combination of higher dose meloxicam (0.6 mg/kg) and a lidocaine/bupivacaine local infusion. DB rabbits showed an increase in RbtGS score at both 1 and 5 h post-surgery. In the NZW rabbits, an increase in RbtGS score was only observed at 1 h post-surgery. Using behavioral analysis as the gold standard for comparison, the RbtGS was an effective means of determining when rabbits are painful following orchidectomy. Higher dose meloxicam (0.6 mg/kg) combined with local anesthetic was a more effective method of reducing pain, compared to lower dose meloxicam (0.2 mg/kg) alone

Synthesis and asymmetric hydrogenation of (3E)-1-benzyl-3-[(2-oxopyridin-1(2H)-yl)methylidene]piperidine-2,6-dione

The synthesis of (3E)-1-benzyl-3-[(2-oxopyridin-1(2H)-yl)methylidene]piperidine-2,6-dione 4 from N-benzylglutarimide was achieved in three steps. The asymmetric hydrogenation of 4 gave either the product of partial reduction (10) or full reduction (13), depending on the catalyst which was employed, in high ee in each case. Attempts at asymmetric transfer hydrogenation (ATH) of 4 resulted in formation of a racemic product