CLAVATA1, a regulator of meristem and flower development in Arabidopsis

We have investigated the effects on plant development of mutations in the Arabidopsis thaliana CLAVATA1 gene. In clavata1 plants, vegetative, inflorescence and floral meristems are all enlarged relative to wild type. The apical meristem can fasciate in the more severe mutant alleles, and this fasciation can occur prior to the transition to flowering. Flowers of clavata1 plants can have increased numbers of organs in all four whorls, and can also have additional whorls not present in wild-type flowers. Double mutant combinations of clavata1 with agamous, apetala2, apetala3 and pistillata indicate that CLAVATA1 controls the underlying floral meristem structure upon which these homeotic genes act. Double mutant combinations of clavata1 with apetala1 and leafy indicate CLAVATA1 plays a role in establishing and maintaining floral meristem identity, in addition to its role in controlling meristem size. In support of this, RNA expression patterns of AGAMOUS and APETALA1 are altered in clavata1 flowers

Extending Foster Care to Age 21: Weighing the Costs to Government against the Benefits to Youth

The Fostering Connections to Success and Increasing Adoptions Act of 2008 allows states to claim federal reimbursement for the costs of caring for and supervising Title IV-E eligible foster youth until their 21st birthday. This issue brief provides preliminary estimates of what the potential costs to government and the benefits to young people would be if states extend foster care to age 21. The analysis focuses on the increase in postsecondary educational attainment associated with allowing foster youth to remain in care until they are 21 years old and the resulting increase in lifetime earnings associated with postsecondary education. Researchers estimate that lifetime earnings would increase an average of two dollars for every dollar spent on keeping foster youth in care beyond age 18

Bacteriological Water Quality of Beaver Reservoir, Arkansas

Beaver Reservoir water quality was determined through enumeration of Total coliforms and Fecal coliforms bacterial parameters at selected locations during 1991. Several areas of the reservoir contained high numbers of indicator bacteria, suggesting excessive fecal contamination. Significant numbers of salmonella-like bacteria were also cultured on SS agar, and several strains were serotyped positive for Salmonella antigens

Increased brain uptake of targeted nanoparticles by adding an acid-cleavable linkage between transferrin and the nanoparticle core

Most therapeutic agents are excluded from entering the central nervous system by the blood–brain barrier (BBB). Receptor mediated transcytosis (RMT) is a common mechanism used by proteins, including transferrin (Tf), to traverse the BBB. Here, we prepared Tf-containing, 80-nm gold nanoparticles with an acid-cleavable linkage between the Tf and the nanoparticle core to facilitate nanoparticle RMT across the BBB. These nanoparticles are designed to bind to Tf receptors (TfRs) with high avidity on the blood side of the BBB, but separate from their multidentate Tf–TfR interactions upon acidification during the transcytosis process to allow release of the nanoparticle into the brain. These targeted nanoparticles show increased ability to cross an in vitro model of the BBB and, most important, enter the brain parenchyma of mice in greater amounts in vivo after systemic administration compared with similar high-avidity nanoparticles containing noncleavable Tf. In addition, we investigated this design with nanoparticles containing high-affinity antibodies (Abs) to TfR. With the Abs, the addition of the acid-cleavable linkage provided no improvement to in vivo brain uptake for Ab-containing nanoparticles, and overall brain uptake was decreased for all Ab-containing nanoparticles compared with Tf-containing ones. These results are consistent with recent reports of high-affinity anti-TfR Abs trafficking to the lysosome within BBB endothelium. In contrast, high-avidity, Tf-containing nanoparticles with the acid-cleavable linkage avoid major endothelium retention by shedding surface Tf during their transcytosis

Evaluation of two unmanned aircraft systems as tools for protecting crops from blackbird damage

In response to a need for mitigating blackbird damage to crops, we evaluated the effectiveness of two unmanned aircraft systems (UAS or drones) to protect crops from blackbird damage. UAS are known to elicit behavioral and physiological responses in wildlife and have been proposed as a means to protect row crop agriculture from avian pest depredation. We evaluated the behavioral responses of captive and free-ranging red-winged blackbirds to a fixed-wing and a rotary-wing (multi-rotor, quadcopter) UAS by comparing preflight behaviors to behaviors during UAS approach. Due to the flight limitations of the respective UAS, the fixed-wing and rotarywing were evaluated at different altitudes. Behavioral responses of captive and free-ranging blackbirds to approaching UAS were categorized as no response, alertness, or attempted escape/flight. Neither captive nor freeranging flocks of red-winged blackbirds displayed behavioral responses to approaches by the fixed-wing UAS when flown at or above 52m above ground level (AGL). However, both captive and free-ranging flocks exhibited behavioral responses to the rotary-wing UAS when flown within 30m AGL. Behavioral responses of blackbirds to the rotary-wing UAS were more pronounced with lower altitude approaches. Our findings suggest that UAS have the potential to modify blackbird behavior in a way that may reduce sunflower crop depredation

A point for comparison: the Flower Garden Banks National Marine Sanctuary

A reoccurring goal listed during the creation of Marine Protected Areas (MPAs) is to return the region to a former state. However, limited data is available that describes or characterizes this former condition. Data collected from ecosystems with comparatively limited anthropogenic impacts, can provide invaluable information in suggesting what former states may have looked like. One example is the Flower Garden Banks National Marine Sanctuary which is located 180 kilometers off the coast of Texas. These relatively isolated and pristine banks are capped by substantial scleractinian coral communities, forming excellent habitat for over 200 species of fish. While fishing is permitted, it is limited by difficulty of access. In 2006, NOAA’s Biogeography Branch, in collaboration with the Sanctuary, initiated the first quantitative assessment of fish resources throughout the diveable portions of the Sanctuary. The sampling design and methodologies employed were identical to those that the Branch has utilized in other more impacted regions of the US Caribbean. Initial analyses reveal that fish density and species richness at the Sanctuary were almost two times greater than that found within the US Caribbean and biomass was approximately six times higher. This was due in large part to the presence of sizeable piscivores of the genera Mycteroperca and Dermatolepis. The Sanctuary is one of few minimally impacted locations remaining within the Tropical Western Atlantic. As such, these findings should be considered when attempting to establish a former state or evaluate effectiveness of an MPA in meeting its management goals

Imaging the phase of an evolving Bose-Einstein condensate wavefunction

We demonstrate a spatially resolved autocorrelation measurement with a Bose-Einstein condensate (BEC) and measure the evolution of the spatial profile of its quantum mechanical phase. Upon release of the BEC from the magnetic trap, its phase develops a form that we measure to be quadratic in the spatial coordinate. Our experiments also reveal the effects of the repulsive interaction between two overlapping BEC wavepackets and we measure the small momentum they impart to each other

Experimental Investigations into the Role of Passive Variable Compliant Legs for Dynamic Robot Locomotion

Biomechanical studies suggest that animals’ abilities to tune their effective leg compliance in response to changing terrain conditions plays an important role in their agile, robust locomotion. However, despite growing interest in leg compliance within the robotics literature, little experimental work has been reported on tunable passive leg compliance in running machines. In this paper we present an empirical study into the role of leg compliance using a composite tunable leg design implemented on our dynamic hexapod, EduBot, with gaits optimized for running speed using a range of leg stiffnesses, on two different surface stiffnesses, and with two different payload configurations (0 kg and 0.91 kg). We found that leg stiffness, surface compliance, and payload had a significant impact on the robot’s final optimized speed and efficiency. These results document the value and efficacy of what we believe is the first autonomous dynamic legged robot capable of runtime leg stiffness adjustment. For more information: Kod*La