Which Triggers Produce the Most Erosive, Frequent, and Longest Runout Turbidity Currents on Deltas?

Subaerial rivers and turbidity currents are the two most voluminous sediment transport processes on our planet, and it is important to understand how they are linked offshore from river mouths. Previously, it was thought that slope failures or direct plunging of river floodwater (hyperpycnal flow) dominated the triggering of turbidity currents on delta fronts. Here we reanalyze the most detailed time‐lapse monitoring yet of a submerged delta; comprising 93 surveys of the Squamish Delta in British Columbia, Canada. We show that most turbidity currents are triggered by settling of sediment from dilute surface river plumes, rather than landslides or hyperpycnal flows. Turbidity currents triggered by settling plumes occur frequently, run out as far as landslide‐triggered events, and cause the greatest changes to delta and lobe morphology. For the first time, we show that settling from surface plumes can dominate the triggering of hazardous submarine flows and offshore sediment fluxes

Preconditioning and triggering of offshore slope failures and turbidity currents revealed by most detailed monitoring yet at a fjord-head delta

Rivers and turbidity currents are the two most important sediment transport processes by volume on Earth. Various hypotheses have been proposed for triggering of turbidity currents offshore from river mouths, including direct plunging of river discharge, delta mouth bar flushing or slope failure caused by low tides and gas expansion, earthquakes and rapid sedimentation. During 2011, 106 turbidity currents were monitored at Squamish Delta, British Columbia. This enables statistical analysis of timing, frequency and triggers. The largest peaks in river discharge did not create hyperpycnal flows. Instead, delayed delta-lip failures occurred 8–11 h after flood peaks, due to cumulative delta top sedimentation and tidally-induced pore pressure changes. Elevated river discharge is thus a significant control on the timing and rate of turbidity currents but not directly due to plunging river water. Elevated river discharge and focusing of river discharge at low tides cause increased sediment transport across the delta-lip, which is the most significant of all controls on flow timing in this setting

The role of synovial macrophages and macrophage-produced cytokines in driving aggrecanases, matrix metalloproteinases, and other destructive and inflammatory responses in osteoarthritis

There is an increasing body of evidence that synovitis plays a role in the progression of osteoarthritis and that overproduction of cytokines and growth factors from the inflamed synovium can influence the production of degradative enzymes and the destruction of cartilage. In this study, we investigate the role of synovial macrophages and their main proinflammatory cytokines, interleukin (IL)-1 and tumour necrosis factor-alpha (TNF-α), in driving osteoarthritis synovitis and influencing the production of other pro- and anti-inflammatory cytokines, production of matrix metalloproteinases, and expression of aggrecanases in the osteoarthritis synovium. We established a model of cultures of synovial cells from digested osteoarthritis synovium derived from patients undergoing knee or hip arthroplasties. By means of anti-CD14-conjugated magnetic beads, specific depletion of osteoarthritis synovial macrophages from these cultures could be achieved. The CD14(+)-depleted cultures no longer produced significant amounts of macrophage-derived cytokines like IL-1 and TNF-α. Interestingly, there was also significant downregulation of several cytokines, such as IL-6 and IL-8 (p < 0.001) and matrix metalloproteinases 1 and 3 (p < 0.01), produced chiefly by synovial fibroblasts. To investigate the mechanisms involved, we went on to use specific downregulation of IL-1 and/or TNF-α in these osteoarthritis cultures of synovial cells. The results indicated that neutralisation of both IL-1 and TNF-α was needed to achieve a degree of cytokine (IL-6, IL-8, and monocyte chemoattractant protein-1) and matrix metalloproteinase (1, 3, 9, and 13) inhibition, as assessed by enzyme-linked immunosorbent assay and by reverse transcription-polymerase chain reaction (RT-PCR), similar to that observed in CD14(+)-depleted cultures. Another interesting observation was that in these osteoarthritis cultures of synovial cells, IL-1β production was independent of TNF-α, in contrast to the situation in rheumatoid arthritis. Using RT-PCR, we also demonstrated that whereas the ADAMTS4 (a disintegrin and metalloprotease with thrombospondin motifs 4) aggrecanase was driven mainly by TNF-α, ADAMTS5 was not affected by neutralisation of IL-1 and/or TNF-α. These results suggest that, in the osteoarthritis synovium, both inflammatory and destructive responses are dependent largely on macrophages and that these effects are cytokine-driven through a combination of IL-1 and TNF-α

Importance of mechanical cues in regulating musculoskeletal circadian clock rhythmicity: implications for articular cartilage

The circadian clock, a collection of endogenous cellular oscillators with an approximate 24‐h cycle, involves autoregulatory transcriptional/translational feedback loops to enable synchronization within the body. Circadian rhythmicity is controlled by a master clock situated in the hypothalamus; however, peripheral tissues are also under the control of autonomous clocks which are coordinated by the master clock to regulate physiological processes. Although light is the primary signal required to entrain the body to the external day, non‐photic zeitgeber including exercise also entrains circadian rhythmicity. Cellular mechano‐sensing is imperative for functionality of physiological systems including musculoskeletal tissues. Over the last decade, mechano‐regulation of circadian rhythmicity in skeletal muscle, intervertebral disc, and bone has been demonstrated to impact tissue homeostasis. In contrast, few publications exist characterizing the influence of mechanical loading on the circadian rhythm in articular cartilage, a musculoskeletal tissue in which loading is imperative for function; importantly, a dysregulated cartilage clock contributes to development of osteoarthritis. Hence, this review summarizes the literature on mechano‐regulation of circadian clocks in musculoskeletal tissues and infers on their collective importance in understanding the circadian clock and its synchronicity for articular cartilage mechanobiology

Glucosamine hydrochloride but not chondroitin sulfate prevents cartilage degradation and inflammation induced by interleukin-1α in bovine cartilage explants

Objective Glucosamine hydrochloride (GH) and chondroitin sulfate (CS) are commonly used for the treatment of osteoarthritis (OA). The aim of this study was to assess their effects, alone and in combination, on preventing aggrecan degradation and inflammation in an in vitro model of OA. Design To test the effects of GH and/or CS as a preventative treatment, cartilage explants were pretreated with the compound(s) using concentrations that showed no detrimental effect on chondrocyte viability. Interleukin-1α (IL-1α) was added to induce cartilage degradation, supernatant and explants were analyzed for proteoglycan degradation products, aggrecanase mRNA expression and activity, and for the release of inflammatory markers. Results Following treatment with IL-1α, 2 mg/mL dose of GH pretreatment was associated with a reduction of glycosaminoglycan release, reduced generation of the pathological interglobular domain aggrecan catabolites, decreased mRNA levels of ADAMTS-4 and -5 and reduced activity of ADAMTS-4. In contrast, CS alone did not have a significant effect on IL-1α-induced cartilage degradation and the addition of 0.4 mg/mL CS to 2 mg/mL GH did not further inhibit IL-1α-induced activity. Pretreatment with 2 mg/mL GH also reduced the release of inflammatory markers, prostaglandin E2 and nitric oxide induced by IL-1α while CS did not have a significant effect. Conclusions The results suggest that GH prevents cartilage degradation mediated by aggrecanases ADAMTS-4 and -5, and may also reduce inflammation. This could be part of the mechanisms by which GH is effective in maintaining joint integrity and function, and preventing or delaying early symptoms of OA

Gas Kinematics on GMC scales in M51 with PAWS: cloud stabilization through dynamical pressure

We use the high spatial and spectral resolution of the PAWS CO(1-0) survey of the inner 9 kpc of the iconic spiral galaxy M51 to examine the effect of gas streaming motions on the star-forming properties of individual GMCs. We compare our view of gas flows in M51 -- which arise due to departures from axi-symmetry in the gravitational potential (i.e. the nuclear bar and spiral arms) -- with the global pattern of star formation as traced by Halpha and 24\mu m emission. We find that the dynamical environment of GMCs strongly affects their ability to form stars, in the sense that GMCs situated in regions with large streaming motions can be stabilized, while similarly massive GMCs in regions without streaming go on to efficiently form stars. We argue that this is the result of reduced surface pressure felt by clouds embedded in an ambient medium undergoing large streaming motions, which prevents collapse. Indeed, the variation in gas depletion time expected based on the observed streaming motions throughout the disk of M51 quantitatively agrees with the variation in observed gas depletion time scale. The example of M51 shows that streaming motions, triggered by gravitational instabilities in the form of bars and spiral arms, can alter the star formation law; this can explain the variation in gas depletion time among galaxies with different masses and morphologies. In particular, we can explain the long gas depletion times in spiral galaxies compared to dwarf galaxies and starbursts. We suggest that adding a dynamical pressure term to the canonical free-fall time produces a single star formation law that can be applied to all star-forming regions and galaxies, across cosmic time.Comment: 28 pages, 14 figures, accepted for publication in Ap

The PdBI Arcsecond Whirlpool Survey (PAWS): Multi-phase cold gas kinematic of M51

The kinematic complexity and the favorable position of M51 on the sky make this galaxy an ideal target to test different theories of spiral arm dynamics. Taking advantage of the new high resolution PdBI Arcsecond Whirlpool Survey (PAWS) data, we undertake a detailed kinematic study of M51 to characterize and quantify the origin and nature of the non-circular motions. Using a tilted-ring analysis supported by several other archival datasets we update the estimation of M51's position angle (PA=(173 +/- 3) deg) and inclination (i=(22 +/- 5) deg). Harmonic decomposition of the high resolution (40 pc) CO velocity field shows the first kinematic evidence of an m=3 wave in the inner disk of M51 with a corotation at R(CR,m=3)=1.1 +/- 0.1 kpc and a pattern speed of Omega_p(m=3) = 140 km/(s kpc). This mode seems to be excited by the nuclear bar, while the beat frequencies generated by the coupling between the m=3 mode and the main spiral structure confirm its density-wave nature. We observe also a signature of an m=1 mode that is likely responsible for the lopsidedness of M51 at small and large radii. We provide a simple method to estimate the radial variation of the amplitude of the spiral perturbation (Vsp) attributed to the different modes. The main spiral arm structure has =50-70 km/s, while the streaming velocity associated with the m=1 and m=3 modes is, in general, 2 times lower. Our joint analysis of HI and CO velocity fields at low and high spatial resolution reveals that the atomic and molecular gas phases respond differently to the spiral perturbation due to their different vertical distribution and emission morphology.Comment: 42 pages, 12 figures, accepted for publication in Ap

The PdBI Arcsecond Whirlpool Survey (PAWS): Environmental Dependence of Giant Molecular Cloud Properties in M51

Using data from the PdBI Arcsecond Whirlpool Survey (PAWS), we have generated the largest extragalactic Giant Molecular Cloud (GMC) catalog to date, containing 1,507 individual objects. GMCs in the inner M51 disk account for only 54% of the total 12CO(1-0) luminosity of the survey, but on average they exhibit physical properties similar to Galactic GMCs. We do not find a strong correlation between the GMC size and velocity dispersion, and a simple virial analysis suggests that 30% of GMCs in M51 are unbound. We have analyzed the GMC properties within seven dynamically-motivated galactic environments, finding that GMCs in the spiral arms and in the central region are brighter and have higher velocity dispersions than inter-arm clouds. Globally, the GMC mass distribution does not follow a simple power law shape. Instead, we find that the shape of the mass distribution varies with galactic environment: the distribution is steeper in inter-arm region than in the spiral arms, and exhibits a sharp truncation at high masses for the nuclear bar region. We propose that the observed environmental variations in the GMC properties and mass distributions are a consequence of the combined action of large-scale dynamical processes and feedback from high mass star formation. We describe some challenges of using existing GMC identification techniques for decomposing the 12CO(1-0) emission in molecule-rich environments, such as M51's inner disk.Comment: 73 pages, 18 figures, 14 tables, accepted for publication in Ap

A Comparative Study of Giant Molecular Clouds in M51, M33 and the Large Magellanic Cloud

We compare the properties of giant molecular clouds (GMCs) in M51 identified by the Plateau de Bure Interferometer Whirlpool Arcsecond Survey (PAWS) with GMCs identified in wide-field, high resolution surveys of CO emission in M33 and the Large Magellanic Cloud (LMC). We find that GMCs in M51 are larger, brighter and have higher velocity dispersions relative to their size than equivalent structures in M33 and the LMC. These differences imply that there are genuine variations in the average mass surface density of the different GMC populations. To explain this, we propose that the pressure in the interstellar medium surrounding the GMCs plays a role in regulating their density and velocity dispersion. We find no evidence for a correlation between size and linewidth in any of M51, M33 or the LMC when the CO emission is decomposed into GMCs, although moderately robust correlations are apparent when regions of contiguous CO emission (with no size limitation) are used. Our work demonstrates that observational bias remains an important obstacle to the identification and study of extragalactic GMC populations using CO emission, especially in molecule-rich galactic environments.Comment: 25 pages, 11 figures, accepted for publication in ApJ. Uses emulateapj LaTeX macros. For more information on PAWS, further papers and data, see http://www.mpia.de/PAWS

A microarray analysis of gene expression in the free-living stages of the parasitic nematode Strongyloides ratti

BACKGROUND: The nematode Strongyloides ratti has two adult phases in its lifecycle: one obligate, female and parasitic and one facultative, dioecious and free-living. The molecular control of the development of this free-living generation remains to be elucidated. RESULTS: We have constructed an S. ratti cDNA microarray and used it to interrogate changes in gene expression during the free-living phase of the S. ratti life-cycle. We have found very extensive differences in gene expression between first-stage larvae (L1) passed in faeces and infective L3s preparing to infect hosts. In L1 stages there was comparatively greater expression of genes involved in growth. We have also compared gene expression in L2 stages destined to develop directly into infective L3s with those destined to develop indirectly into free-living adults. This revealed relatively small differences in gene expression. We find little evidence for the conservation of transcription profiles between S. ratti and S. stercoralis or C. elegans. CONCLUSION: This is the first multi-gene study of gene expression in S. ratti. This has shown that robust data can be generated, with consistent measures of expression within computationally determined clusters and contigs. We find inconsistencies between EST representation data and microarray hybridization data in the identification of genes with stage-specific expression and highly expressed genes. Many of the genes whose expression is significantly different between L1 and iL3s stages are unknown beyond alignments to predicted genes. This highlights the forthcoming challenge in actually determining the role of these genes in the life of S. ratti