Towards a standardization of biomethane potential tests

Production of biogas from different organic materials is a most interesting source of renewable energy. The biomethane potential (BMP) of these materials has to be determined to get insight in design parameters for anaerobic digesters. Although several norms and guidelines for BMP tests exist, inter-laboratory tests regularly show high variability of BMPs for the same substrate. A workshop was held in June 2015, in Leysin, Switzerland, with over 40 attendees from 30 laboratories around the world, to agree on common solutions to the conundrum of inconsistent BMP test results. This paper presents the consensus of the intense roundtable discussions and cross-comparison of methodologies used in respective laboratories. Compulsory elements for the validation of BMP results were defined. They include the minimal number of replicates, the request to carry out blank and positive control assays, a criterion for the test duration, details on BMP calculation, and last but not least criteria for rejection of the BMP tests. Finally, recommendations on items that strongly influence the outcome of BMP tests such as inoculum characteristics, substrate preparation, test setup, and data analysis are presented to increase the probability of obtaining validated and reproducible results.info:eu-repo/semantics/publishedVersio

Optimisation du procédé de méthanisation par mise en place d'un co-traitement physico-chimique (application au gisement de biogaz représenté par les boues d'épuration des eaux usées)

MONTPELLIER-BU Sciences (341722106) / SudocSudocFranceF

Traitements physico-chimiques des boues d'épuration en vue de leur valorisation par méthanisation

National audienc

Results from a French Inter‐laboratory Campaign on the Biological Methane Potential of Solid Substrates

International audienceA French inter-laboratory campaign on BMP assessment was realised in two phases: free protocol at first, then harmonized protocol. Results show good intra-laboratory repeatability and reproducibility, but in spite of an attempt in the harmonization of practices, the inter-laboratoryreproducibility could not be improved and ranges in the magnitude of 20% RSD

Results from a French Inter‐laboratory Campaign on the Biological Methane Potential of Solid Substrates

International audienceA French inter-laboratory campaign on BMP assessment was realised in two phases: free protocol at first, then harmonized protocol. Results show good intra-laboratory repeatability and reproducibility, but in spite of an attempt in the harmonization of practices, the inter-laboratoryreproducibility could not be improved and ranges in the magnitude of 20% RSD

Results from a French Inter‐laboratory Campaign on the Biological Methane Potential of Solid Substrates

International audienc

Results from a french inter‐laboratory campaign on the biological methane potential of solid substrates

International audienceEvery year the knowledge about the microbial ecology of anaerobic digestion increases but despite that, the inoculation step of anaerobic digesters still remains empirical. Anaerobic digestion remains a microbial process without any microbial engineering. In this review we try to understand why putting in practice this knowledge remains difficult by identifying bottlenecks of this unsuccessful story

Results from a French Inter‐laboratory Campaign on the Biological Methane Potential of Solid Substrates

International audienc

Measurement of Biochemical Methane Potential of Heterogeneous Solid Substrates: Results of a Two- Phase French Inter-Laboratory Study

International audienceBiochemical methane potential (BMP) is essential to determine the production of methane for various substrates; literature shows important discrepancies for the same substrates. In this paper, a harmonized BMP protocol was developed and tested with two phases of BMP tests carried out by eleven French laboratories. Surprisingly, for the three same solid tested substrates (straw; raw mix and dried-shredded mix of potatoes, maize, beef meat and straw; and mayonnaise), the standard deviations of the repeatability and reproducibility inter-laboratory were not enhanced by the harmonized protocol (average of about 25% depending on the substrate), as compared to a previous step where all laboratories used their own protocols. Moreover, statistical analyses of all the results, after removal of the outliers (about 15% of all observations), did not highlight significant effect of the operational effect on BMP (stirring, automatic or manual gas quantification, use of trace metal, uses a bicarbonate buffer, inoculum to substrate ratio) at least for the tested ranges. On the other hand, the average intra-laboratory repeatability was low, about 7%, whatever the protocol, the substrate and the laboratory. It also appears that drying the SA substrate, which contained proteins, carbohydrates, lipids and fibers, does not impact its BMP