Stability of axial orbits in galactic potentials

We investigate the dynamics in a galactic potential with two reflection symmetries. The phase-space structure of the real system is approximated with a resonant detuned normal form constructed with the method based on the Lie transform. Attention is focused on the stability properties of the axial periodic orbits that play an important role in galactic models. Using energy and ellipticity as parameters, we find analytical expressions of bifurcations and compare them with numerical results available in the literature.Comment: 20 pages, accepted for publication on Celestial Mechanics and Dynamical Astronom

Tailoring lipoplex composition to the lipid composition of plasma membrane: a Trojan horse for cell entry?

The first interaction between lipoplexes and cells is charge-mediated and not specific. Endocytosis is considered to be the main pathway for lipoplex entry. Upon interaction between lipoplexes and the plasma membrane, intermixing between lipoplex and membrane lipids is necessary for efficient endocytosis. Here we study the mechanism of the different endocytic pathways in lipid-mediated gene delivery. We show that DC-Chol-DOPE/DNA lipoplexes preferentially use a raftmediated endocytosis, while DOTAP-DOPC/DNA systems are mainly internalized by not specific fluid phase macropinocitosys. On the other hand, most efficient multicomponent lipoplexes, incorporating different lipid species in their lipid bilayer, can use multiple endocytic pathways to enter cells.Our data demonstrate that efficiency of endocytosis is regulated by shape coupling between lipoplex and membrane lipids. We suggest that such a shape-dependent coupling regulates efficient formation of endocytic vesicles thus determining the success of internalization. Our results suggest that tailoring the lipoplex lipid composition to the patchwork-like plasma membrane profile could be a successful machinery of coordinating the endocytic pathway activities and the subsequent intracellular processing

Friend or foe? The current epidemiologic evidence on selenium and human cancer risk.

Scientific opinion on the relationship between selenium and the risk of cancer has undergone radical change over the years, with selenium first viewed as a possible carcinogen in the 1940s then as a possible cancer preventive agent in the 1960s-2000s. More recently, randomized controlled trials have found no effect on cancer risk but suggest possible low-dose dermatologic and endocrine toxicity, and animal studies indicate both carcinogenic and cancer-preventive effects. A growing body of evidence from human and laboratory studies indicates dramatically different biological effects of the various inorganic and organic chemical forms of selenium, which may explain apparent inconsistencies across studies. These chemical form-specific effects also have important implications for exposure and health risk assessment. Overall, available epidemiologic evidence suggests no cancer preventive effect of increased selenium intake in healthy individuals and possible increased risk of other diseases and disorders

Domain wall dynamics and Barkhausen effect in metallic ferromagnetic materials. II. Experiments

Barkhausen effect (BE) phenomenology in iron‐based ferromagnetic alloys is investigated by a proper experimental method, in which BE experiments are restricted to the central part of the hysteresis loop, and the amplitude probability distribution, P0(Φ), and power spectrum, F(ω), of the B flux rate Φ are measured under controlled values of the magnetization rate and differential permeability μ. It is found that all of the experimental data are approximately consistent with the law P0(Φ)∝Φ−1 exp(−Φ/〈Φ〉), where all dependencies on and μ are described by the single dimensionless parameter >0. Also the parameters describing the shape of F(ω) are found to obey remarkably simple and general laws of dependence on and μ. The experimental results are interpreted by means of the Langevin theory of domain‐wall dynamics proposed in a companion paper. The theory is in good agreement with experiments, and permits one to reduce the basic aspects of BE phenomenology to the behavior of two parameters describing the stochastic fluctuations of the local coercive field experienced by a moving domain wall

Correction: miRNAs in the vitreous humor of patients affected by idiopathic epiretinal membrane and macular hole

[This corrects the article DOI: 10.1371/journal.pone.0174297.]

HIPK2 and extrachromosomal histone H2B are separately recruited by Aurora-B for cytokinesis

Cytokinesis, the final phase of cell division, is necessary to form two distinct daughter cells with correct distribution of genomic and cytoplasmic materials. Its failure provokes genetically unstable states, such as tetraploidization and polyploidization, which can contribute to tumorigenesis. Aurora-B kinase controls multiple cytokinetic events, from chromosome condensation to abscission when the midbody is severed. We have previously shown that HIPK2, a kinase involved in DNA damage response and development, localizes at the midbody and contributes to abscission by phosphorylating extrachromosomal histone H2B at Ser14. Of relevance, HIPK2-defective cells do not phosphorylate H2B and do not successfully complete cytokinesis leading to accumulation of binucleated cells, chromosomal instability, and increased tumorigenicity. However, how HIPK2 and H2B are recruited to the midbody during cytokinesis is still unknown. Here, we show that regardless of their direct (H2B) and indirect (HIPK2) binding of chromosomal DNA, both H2B and HIPK2 localize at the midbody independently of nucleic acids. Instead, by using mitotic kinase-specific inhibitors in a spatio-temporal regulated manner, we found that Aurora-B kinase activity is required to recruit both HIPK2 and H2B to the midbody. Molecular characterization showed that Aurora-B directly binds and phosphorylates H2B at Ser32 while indirectly recruits HIPK2 through the central spindle components MgcRacGAP and PRC1. Thus, among different cytokinetic functions, Aurora-B separately recruits HIPK2 and H2B to the midbody and these activities contribute to faithful cytokinesis