On Superdeterministic Rejections of Settings Independence

Relying on some auxiliary assumptions, usually considered mild, Bell's theorem proves that no local theory can reproduce all the predictions of quantum mechanics. In this work, we introduce a fully local, superdeterministic model that, by explicitly violating settings independence--one of these auxiliary assumptions, requiring statistical independence between measurement settings and systems to be measured--is able to reproduce all the predictions of quantum mechanics. Moreover, we show that, contrary to widespread expectations, our model can break settings independence without an initial state that is too complex to handle, without visibly losing all explanatory power and without outright nullifying all of experimental science. Still, we argue that our model is unnecessarily complicated and does not offer true advantages over its non-local competitors. We conclude that, while our model does not appear to be a viable contender to their non-local counterparts, it provides the ideal framework to advance the debate over violations of statistical independence via the superdeterministic route

Simultaneous Multi-Harmonic Imaging of Nanoparticles in Tissues for Increased Selectivity

We investigate the use of Bismuth Ferrite (BFO) nanoparticles for tumor tissue labelling in combination with infrared multi-photon excitation at 1250 nm. We report the efficient and simultaneous generation of second and third harmonic by the nanoparticles. On this basis, we set up a novel imaging protocol based on the co-localization of the two harmonic signals and demonstrate its benefits in terms of increased selectivity against endogenous background sources in tissue samples. Finally, we discuss the use of BFO nanoparticles as mapping reference structures for correlative light-electron microscopy.Comment: 19 pages, 6 figure

Media 2: PSF shaping using adaptive optics for three-dimensional single-molecule super-resolution imaging and tracking

Originally published in Optics Express on 27 February 2012 (oe-20-5-4957

Media 4: PSF shaping using adaptive optics for three-dimensional single-molecule super-resolution imaging and tracking

Originally published in Optics Express on 27 February 2012 (oe-20-5-4957

Media 1: PSF shaping using adaptive optics for three-dimensional single-molecule super-resolution imaging and tracking

Originally published in Optics Express on 27 February 2012 (oe-20-5-4957

High-speed tracking of murine cardiac stem cells by harmonic nanodoublers

Potassium niobate nonlinear nanoparticles are used for the first time to monitor the evolution of embryonic stem cells (ESC) by second harmonic microscopy. These particles feature the complete absence of photo-bleaching and unlimited excitation wavelength flexibility. The potential of this approach is made evident for tissue-regeneration studies and applications, by capturing a high-speed movie of ESC-derived cardiomyocytes autonomously beating within a cluster. Time-resolved data are analyzed to retrieve 3D information of the contraction pattern at the cellular level

Harmonic nanoparticles for regenerative research

In this visualized experiment, protocol details are provided for in vitro labeling of human embryonic stem cells (hESC) with second harmonic generation nanoparticles (HNPs). The latter are a new family of probes recently introduced for labeling biological samples for multi-photon imaging. HNPs are capable of doubling the frequency of excitation light by the nonlinear optical process of second harmonic generation with no restriction on the excitation wavelength. Multi-photon based methodologies for hESC differentiation into cardiac clusters (maintained as long term air-liquid cultures) are presented in detail. In particular, evidence on how to maximize the intense second harmonic (SH) emission of isolated HNPs during 3D monitoring of beating cardiac tissue in 3D is shown. The analysis of the resulting images to retrieve 3D displacement patterns is also detailed

Harmonic nanocrystals for biolabeling: a survey of optical properties and biocompatibility.

International audienceNonlinear optical nanocrystals have been recently introduced as a promising alternative to fluorescent probes for multiphoton microscopy. We present for the first time a complete survey of the properties of five nanomaterials (KNbO3, LiNbO3, BaTiO3, KTP, and ZnO), describing their preparation and stabilization and providing quantitative estimations of their nonlinear optical response. In the light of their prospective use as biological and clinical markers, we assess their biocompatibility on human healthy and cancerous cell lines. Finally, we demonstrate the great potential for cell imaging of these inherently nonlinear probes in terms of optical contrast, wavelength flexibility, and signal photostability