Relationship Between Anti-DFS70 Autoantibodies and Oxidative Stress

Background: The anti-DFS70 autoantibodies are one of the most commonly and widely described agent of unknown clinical significance, frequently detected in healthy individuals. It is not known whether the DFS70 autoantibodies are protective or pathogenic. One of the factors suspected of inducing the formation of anti-DFS70 antibodies is increased oxidative stress. We evaluated the coexistence of anti-DFS70 antibodies with selected markers of oxidative stress and investigated whether these antibodies could be considered as indirect markers of oxidative stress. Methods: The intensity of oxidative stress was measured in all samples via indices of free-radical damage to lipids and proteins such as total oxidant status (TOS), concentrations of lipid hydroperoxides (LPH), lipofuscin (LPS), and malondialdehyde (MDA). The parameters of the non-enzymatic antioxidant system, such as total antioxidant status (TAS) and uric acid concentration (UA), were also measured, as well as the activity of superoxide dismutase (SOD). Based on TOS and TAS values, the oxidative stress index (OSI) was calculated. All samples were also tested with indirect immunofluorescence assay (IFA) and 357 samples were selected for direct monospecific anti DFS70 enzyme-linked immunosorbent assay (ELISA) testing. Results: The anti-DFS70 antibodies were confirmed by ELISA test in 21.29% of samples. Compared with anti-DFS70 negative samples we observed 23% lower concentration of LPH (P =.038) and 11% lower concentration of UA (P =.005). TOS was 20% lower (P =.014). The activity of SOD was up to 5% higher (P =.037). The Pearson correlation showed weak negative correlation for LPH, UA, and TOS and a weak positive correlation for SOD activity. Conclusion: In samples positive for the anti-DFS70 antibody a decreased level of oxidative stress was observed, especially in the case of samples with a high antibody titer. Anti-DFS70 antibodies can be considered as an indirect marker of reduced oxidative stress or a marker indicating the recent intensification of antioxidant processes

Analysis of the impact of sex and age on the variation in the prevalence of antinuclear autoantibodies in Polish population: a nationwide observational, cross-sectional study

The detection of antinuclear autoantibody (ANA) is dependent on many factors and varies between the populations. The aim of the study was first to assess the prevalence of ANA in the Polish adult population depending on age, sex and the cutoff threshold used for the results obtained. Second, we estimated the occurrence of individual types of ANA-staining patterns. We tested 1731 patient samples using commercially available IIFA using two cutoff thresholds of 1:100 and 1:160. We found ANA in 260 participants (15.0%), but the percentage of positive results strongly depended on the cutoff level. For a cutoff threshold 1:100, the positive population was 19.5% and for the 1:160 cutoff threshold, it was 11.7%. The most prevalent ANA-staining pattern was AC-2 Dense Fine speckled (50%), followed by AC-21 Reticular/AMA (14.38%) ANA more common in women (72%); 64% of ANA-positive patients were over 50 years of age. ANA prevalence in the Polish population is at a level observed in other highly developed countries and is more prevalent in women and elderly individuals. To reduce the number of positive results released, we suggest that Polish laboratories should set 1:160 as the cutoff threshold

Monolithic carbon dioxide hybrid adsorbents

Opracowano nowe strukturalne sorbenty ditlenku węgla oparte na poliaminach osadzonych w krzemionkowych monolitycznych materiałach o bimodalnej strukturze porów. Zbadano zależność pojemności sorpcyjnej od rodzaju i ilości wprowadzonych prekursorów amin. Otrzymane wyniki porównano z danymi literaturowymi sorpcji CO2 na podobnie zmodyfikowanych proszkowych, porowatych materiałach. Zaproponowane nośniki, poza zdecydowanie lepszą użytkową formę, wykazywały lepsze właściwości sorpcyjne.New structural carbon dioxide sorbents based on supported polyamines onto silica monoliths with hierarchical pore structure were elaborated. Sorption capacity/type/amount of amine precursor relationships were studied. The results obtained were compared with the literature data relating to the sorption of CO2 on similarly modified powdered porous materials. The proposed carriers, in addition to a more usable form, showed improved sorption properties

Effective palladium-functionalized catalysts for Suzuki coupling reaction

Opracowano efektywne, heterogeniczne katalizatory funkcjonalizowane organicznymi kompleksami palladu do reakcji sprzęgania Suzukiego z wykorzystaniem monolitycznych krzemionkowych materiałów o hierarchicznej strukturze porów oraz mezostrukturalnego nośnika typu SBA-15. Właściwości materiałów zbadano za pomocą SEM, TEM, adsorpcji azotu, FTIR oraz TG. Wysoka aktywność katalizatorów została potwierdzona w reakcji jodobenzenu z kwasem fenyloboronowym. W procesie prowadzonym w przepływowym mikroreaktorze uzyskano konwersję 96% i stwierdzono stabilność właściwości katalitycznych.Effective heterogeneous catalysts for the Suzuki coupling reaction have been prepared. Catalysts based on silica monoliths with hierarchical pore structure and SBA-15 modified with palladium organic complexes. Transmission electron microscopy (TEM), nitrogen physical adsorption, thermogravimetry (TG) and Fourier transform infrared (FTIR) studies have been used to characterize the materials. Catalysts exhibited high activity for Suzuki coupling reaction of iodobenzene with phenylboronic acid. The flow microreactor showed stability of catalytic properties with an average conversion of 96%

Comparative study of continuous-flow microreactors based on silica monoliths modified with lewis acid centres

Silica multichannel monoliths modified with zirconia, titania and alumina have been used as reactive cores of microreactors and studied in chemoselective reduction (MPV) of cyclohexanon/benzaldehyde with 2-butanol as a hydrogen donor. The attachment of metal oxides to the silica surface was confirmed by FT–IR spectroscopy, and dispersion of metal oxides was studied by UV–Vis spectroscopy. the catalytic activity of the lewis acid centres in both chemical processes decreased in the order zirconia > alumina > titania. This activity is in good agreement with dispersion and coordination of metal species. good stability of zirconia-grafted reactors was confirmed. high porosity of the monoliths and the presence of large meandering flow-through channels with a diameter of ca. 30 mm facilitate fluid transport and very effective mixing in the microreactors. The whole synthesis process is perfectly in line with trends of modern flow chemistry

Review on hierarchically microstructured monolithic reactors for high yield continuous production of fine chemicals

Preparation and properties of hierarchically structured porous silica monoliths have been discussed from the viewpoint of their application as continuous microreactors for liquid-phase synthesis of fine chemical in multi kilogram scales. The results of recent topical papers published by two research teams of Institute of Chemical Engineering Polish Academy of Sciences (ICE) and Department of Chemical Engineering and Process Design, Chemical Faculty, Silesian University of Technology (SUT) have been analyzed to specify the governing traits of microreactors. It was concluded that even enhancement factor of 100 in activity, seen in enzyme catalyzed reactions, can be explained by a proportional reduction of its physical constraints, i.e. huge enhancement of external mass transfer and micromixing. It is induced by very chaotic flows of liquid in tens of thousands of waving connected channels of ca. 25–50 mm in diameter, present in the skeleton. The scale of enhancement in the caseof less active catalysts was smaller, but still large enough to consider the most practical applications

Metabolic syndrome is associated with similar long-term prognosis in non-obese and obese patients. An analysis of 45 615 patients from the nationwide LIPIDOGRAM 2004-2015 cohort studies

Aims We aimed to evaluate the association between metabolic syndrome (MetS) and long-term all-cause mortality. Methods The LIPIDOGRAM studies were carried out in the primary care in Poland in 2004, 2006 and 2015. MetS was diagnosed based on the National Cholesterol Education Program, Adult Treatment Panel III (NCEP/ATP III) and Joint Interim Statement (JIS) criteria. The cohort was divided into four groups: non-obese patients without MetS, obese patients without MetS, non-obese patients with MetS and obese patients with MetS. Differences in all-cause mortality was analyzed using Kaplan-Meier and Cox regression analyses. Results 45,615 participants were enrolled (mean age 56.3, standard deviation: 11.8 years; 61.7% female). MetS was diagnosed in 14,202 (31%) by NCEP/ATP III criteria, and 17,216 (37.7%) by JIS criteria. Follow-up was available for 44,620 (97.8%, median duration 15.3 years) patients. MetS was associated with increased mortality risk among the obese (hazard ratio, HR: 1.88 [95% CI, 1.79-1.99] and HR: 1.93 [95% CI 1.82-2.04], according to NCEP/ATP III and JIS criteria, respectively) and non-obese individuals (HR: 2.11 [95% CI 1.85-2.40] and 1.7 [95% CI, 1.56-1.85] according to NCEP/ATP III and JIS criteria respectively). Obese patients without MetS had a higher mortality risk than non-obese patients without MetS (HR: 1.16 [95% CI 1.10-1.23] and HR: 1.22 [95%CI 1.15-1.30], respectively in subgroups with NCEP/ATP III and JIS criteria applied). Conclusions MetS is associated with increased all-cause mortality risk in non-obese and obese patients. In patients without MetS obesity remains significantly associated with mortality. The concept of metabolically healthy obesity should be revised

Relationship Between Anti-DFS70 Autoantibodies and Oxidative Stress

Background: The anti-DFS70 autoantibodies are one of the most commonly and widely described agent of unknown clinical significance, frequently detected in healthy individuals. It is not known whether the DFS70 autoantibodies are protective or pathogenic. One of the factors suspected of inducing the formation of anti-DFS70 antibodies is increased oxidative stress. We evaluated the coexistence of anti-DFS70 antibodies with selected markers of oxidative stress and investigated whether these antibodies could be considered as indirect markers of oxidative stress. Methods: The intensity of oxidative stress was measured in all samples via indices of free-radical damage to lipids and proteins such as total oxidant status (TOS), concentrations of lipid hydroperoxides (LPH), lipofuscin (LPS), and malondialdehyde (MDA). The parameters of the non-enzymatic antioxidant system, such as total antioxidant status (TAS) and uric acid concentration (UA), were also measured, as well as the activity of superoxide dismutase (SOD). Based on TOS and TAS values, the oxidative stress index (OSI) was calculated. All samples were also tested with indirect immunofluorescence assay (IFA) and 357 samples were selected for direct monospecific anti DFS70 enzyme-linked immunosorbent assay (ELISA) testing. Results:: The anti-DFS70 antibodies were confirmed by ELISA test in 21.29% of samples. Compared with anti-DFS70 negative samples we observed 23% lower concentration of LPH (P = .038) and 11% lower concentration of UA (P = .005). TOS was 20% lower (P = .014). The activity of SOD was up to 5% higher (P = .037). The Pearson correlation showed weak negative correlation for LPH, UA, and TOS and a weak positive correlation for SOD activity. Conclusion: In samples positive for the anti-DFS70 antibody a decreased level of oxidative stress was observed, especially in the case of samples with a high antibody titer. Anti-DFS70 antibodies can be considered as an indirect marker of reduced oxidative stress or a marker indicating the recent intensification of antioxidant processes