file3. measurements on selected trichomes

This table contains measurements on the selected trichomes with consistent diameter (67–70 μm). From these data, we can get the positive linear correlation of complete cross-walls spacing and the intercalated incomplete cross-walls length

file2. Parallel to bedding

The table contains series of measurements for Megathrix longus in thin sections parallel to the bedding

Analysis of association of SNP rs9943582 with echocardiographic parameters.

<p>CAD: coronary artery disease, LV: left ventricle, LVSD: left ventricle systolic dysfunction, LVEF: left ventricle eject fraction.</p><p>Analysis of association of SNP rs9943582 with echocardiographic parameters.</p

Characteristics of the study population for CAD.

<p>Data are shown as mean +/- standard deviation (SD) for quantitative variables and % for qualitative variables.</p><p>CAD: coronary artery disease; HDL-c: high density lipoprotein cholesterol levels; LDL-c, low density lipoprotein cholesterol levels; n.a: no data</p><p>*Age at the first diagnosis of the disease in CAD cases and age at enrollment for CAD controls.</p><p>^†<i>P</i> value for comparison of means for quantitative data with a student t-test, and for comparison of distribution of qualitative variables between cases and controls with a Chi-square test.</p><p>Characteristics of the study population for CAD.</p

Characteristics of the CAD group with LVSD and the CAD group with normal LVEF.

<p>Data are shown as mean +/- standard deviation (SD) for quantitative variables and % for qualitative variables.</p><p>CAD: coronary artery disease, HDL-c: high density lipoprotein cholesterol levels; LDL-c, low density lipoprotein cholesterol levels, LVSD: left ventricle systolic dysfunction, LVEF: left ventricle eject fraction.</p><p>*Age at the first diagnosis of the disease for CAD cases and age at enrollment for CAD controls.</p><p>^†<i>P</i> values for comparison of means for quantitative data with a student t-test, and for comparison of distribution of qualitative variables between CAD patients with LVSD and CAD patients with normal LVEF with a Chi-square test.</p><p>Characteristics of the CAD group with LVSD and the CAD group with normal LVEF.</p

Analysis of Allelic Association of SNP rs9943582 with LVSD among the CAD population.

<p>Freq _A: Frequency of A allele. LVSD: left ventricle systolic dysfunction, LVEF: left ventricle eject fraction.</p><p>*Uncorrected <i>P</i> value and odds ratio (OR) using Chi-square tests with Pearson’s 2×2.</p><p>^†Adjusted <i>P</i> value by multivariate logistic regression analysis for potential confounders including age, gender, smoking, hypertension, diabetes mellitus and lipid concentrations (Tch, TG, HDL-c and LDL-c).</p><p>^‡ Empirical P values were calculated using 100,000 Monte Carlo simulations</p><p>^‖Multiple testing was adjusted by Bonferroni correction</p><p>Analysis of Allelic Association of SNP rs9943582 with LVSD among the CAD population.</p

<i>PITX2c</i> and <i>ZFHX3</i> regulate expression of <i>NKX2</i>.<i>5</i>, <i>TBX5</i>, <i>KCNQ1</i> and <i>SCN1B</i>.

<p>HCT116 cells were transfected with siRNA specific for <i>PITX2c</i> or <i>ZFHX3</i> and used for isolation of total RNA samples and real-time RT-PCR analysis. Transfection of siRNA for <i>PITX2c</i> increased expression of <i>NKX2</i>.<i>5</i>, <i>TBX5</i>, <i>KCNQ1</i> and <i>SCN1B</i>. Transfection of siRNA for <i>ZFHX3</i> increased expression of <i>NKX2</i>.<i>5</i> and <i>TBX5</i>,but decreased expression of <i>SCN1B</i>. <i>ZFHX3</i> did not affect on the expression of <i>KCNQ1</i>. Transfection of siRNAs for both <i>PITX2c</i> and <i>ZFHX3</i> increased expression of <i>NKX2</i>.<i>5</i> and <i>TBX5</i>.</p

The <i>ZFHX3</i> gene positively regulates expression of the <i>PITX2c</i> gene.

<p>HCT116 cells were transfected with siRNA specific for <i>ZFHX3</i> or an expression plasmid for <i>ZFHX3</i> and used for isolation of total RNA samples, real time RT-PCR analysis and Luciferase assays. A. Real-time RT-PCR analysis for <i>ZFHX3</i>. Transfection of siRNA for <i>ZFHX3</i> successfully reduced expression of <i>ZFHX3</i>. B. Real-time RT-PCR analysis for <i>ZFHX3</i>. Transfection of an expression plasmid for <i>ZFHX3</i> successfully increased expression of <i>ZFHX3</i>. C. Real-time RT-PCR analysis for <i>PITX2c</i>. Transfection of siRNA for <i>ZFHX3</i> reduced expression of <i>PITX2c</i>. D. Real-time RT-PCR analysis for <i>PITX2c</i>. Transfection of an expression plasmid for <i>ZFHX3</i> successfully increased expression of <i>PITX2c</i>. E. Luciferase assays for the <i>PITX2c</i> promoter activity in cells transfected with a siRNA specific for <i>ZFHX3</i> or a control scramble siRNA. *<i>P</i><0.05; **<i>P</i><0.01.</p

A schematic diagram showing the cyclic cross-regulation of <i>PITX2c</i> and <i>ZFHX3</i>, which underlies the interaction between <i>PITX2c</i> and <i>ZFHX3</i> involved in the pathogenesis of AF.

<p>A schematic diagram showing the cyclic cross-regulation of <i>PITX2c</i> and <i>ZFHX3</i>, which underlies the interaction between <i>PITX2c</i> and <i>ZFHX3</i> involved in the pathogenesis of AF.</p

Both <i>PITX2c</i> and <i>ZFHX3</i> positively regulate expression of the <i>NPPA</i> transcription.

<p>HCT116 cells were co-transfected with an expression plasmid for either <i>PITX2c</i>, <i>ZFHX3</i> or both, or siRNA for either <i>PITX2c</i>, <i>ZFHX3</i> or both and used for measurements of RT-PCR. A. Knockdown of <i>PITX2c</i>, <i>ZFHX3</i> or both by siRNAs down-regulated <i>NPPA</i> expression. B. Overexpression of either <i>PITX2c</i> or <i>ZFHX3</i> up-regulated <i>NPPA</i> expression. Co-expression of both <i>PITX2c</i> and <i>ZFHX3</i> dramatically increased <i>NPPA</i> expression. *<i>P</i><0.05; **<i>P</i><0.01.</p