Infrared effects in inflationary correlation functions

In this article, I briefly review the status of infrared effects which occur when using inflationary models to calculate initial conditions for a subsequent hot, dense plasma phase. Three types of divergence have been identified in the literature: secular, "time-dependent" logarithms, which grow with time spent outside the horizon; "box-cutoff" logarithms, which encode a dependence on the infrared cutoff when calculating in a finite-sized box; and "quantum" logarithms, which depend on the ratio of a scale characterizing new physics to the scale of whatever process is under consideration, and whose interpretation is the same as conventional field theory. I review the calculations in which these divergences appear, and discuss the methods which have been developed to deal with them.Comment: Invited review for focus section of Classical & Quantum Gravity on nonlinear and nongaussian perturbation theory. Some improvements compared to version which will appear in CQG, especially in Sec. 2.3. 30 pages + references

Consistent perturbations in an imperfect fluid

We present a new prescription for analysing cosmological perturbations in a more-general class of scalar-field dark-energy models where the energy-momentum tensor has an imperfect-fluid form. This class includes Brans-Dicke models, f(R) gravity, theories with kinetic gravity braiding and generalised galileons. We employ the intuitive language of fluids, allowing us to explicitly maintain a dependence on physical and potentially measurable properties. We demonstrate that hydrodynamics is not always a valid description for describing cosmological perturbations in general scalar-field theories and present a consistent alternative that nonetheless utilises the fluid language. We apply this approach explicitly to a worked example: k-essence non-minimally coupled to gravity. This is the simplest case which captures the essential new features of these imperfect-fluid models. We demonstrate the generic existence of a new scale separating regimes where the fluid is perfect and imperfect. We obtain the equations for the evolution of dark-energy density perturbations in both these regimes. The model also features two other known scales: the Compton scale related to the breaking of shift symmetry and the Jeans scale which we show is determined by the speed of propagation of small scalar-field perturbations, i.e. causality, as opposed to the frequently used definition of the ratio of the pressure and energy-density perturbations.Comment: 40 pages plus appendices. v2 reflects version accepted for publication in JCAP (new summary of notation, extra commentary on choice of gauge and frame, extra references to literature

Pure kinetic k-essence as the cosmic speed-up

In this paper, we consider three types of k-essence. These k-essence models were presented in the parametric forms. The exact analytical solutions of the corresponding equations of motion are found. It is shown that these k-essence models for the presented solutions can give rise to cosmic acceleration.Comment: 10 pages, typos corrected, main results remain the same, minor changes to match IJTP accepted versio

Nitric Oxide Mediates Stretch-Induced Ca2+ Release via Activation of Phosphatidylinositol 3-Kinase-Akt Pathway in Smooth Muscle

Hollow smooth muscle organs such as the bladder undergo significant changes in wall tension associated with filling and distension, with attendant changes in muscle tone. Our previous study indicated that stretch induces Ca(2+) release occurs in the form of Ca(2+) sparks and Ca(2+) waves in urinary bladder myocytes. While, the mechanism underlying stretch-induced Ca2+ release in smooth muscle is unknown.We examined the transduction mechanism linking cell stretch to Ca(2+) release. The probability and frequency of Ca(2+) sparks induced by stretch were closely related to the extent of cell extension and the time that the stretch was maintained. Experiments in tissues and single myocytes indicated that mechanical stretch significantly increases the production of nitric oxide (NO) and the amplitude and duration of muscle contraction. Stretch-induced Ca(2+) sparks and contractility increases were abrogated by the NO inhibitor L-NAME and were also absent in eNOS knockout mice. Furthermore, exposure of eNOS null mice to exogenously generated NO induced Ca(2+) sparks. The soluble guanylyl cyclase inhibitor ODQ did not inhibit SICR, but this process was effectively blocked by the PI3 kinase inhibitors LY494002 and wortmannin; the phosphorylation of Akt and eNOS were up-regulated by 204+/-28.6% and 258+/-36.8% by stretch, respectively. Moreover, stretch significantly increased the eNOS protein expression level.Taking together, these results suggest that stretch-induced Ca2+ release is NO dependent, resulting from the activation of PI3K/Akt pathway in smooth muscle

Weak lensing generated by vector perturbations and detectability of cosmic strings

We study the observational signature of vector metric perturbations through the effect of weak gravitational lensing. In the presence of vector perturbations, the non-vanishing signals for B-mode cosmic shear and curl-mode deflection angle, which have never appeared in the case of scalar metric perturbations, naturally arise. Solving the geodesic and geodesic deviation equations, we drive the full-sky formulas for angular power spectra of weak lensing signals, and give the explicit expressions for E-/B-mode cosmic shear and gradient-/curl-mode deflection angle. As a possible source for seeding vector perturbations, we then consider a cosmic string network, and discuss its detectability from upcoming weak lensing and CMB measurements. Based on the formulas and a simple model for cosmic string network, we calculate the angular power spectra and expected signal-to-noise ratios for the B-mode cosmic shear and curl-mode deflection angle. We find that the weak lensing signals are enhanced for a smaller intercommuting probability of the string network, $P$, and they are potentially detectable from the upcoming cosmic shear and CMB lensing observations. For $P\sim 10^{-1}$, the minimum detectable tension of the cosmic string will be down to $G\mu\sim 5\times 10^{-8}$. With a theoretically inferred smallest value $P\sim 10^{-3}$, we could even detect the string with $G\mu\sim 5\times 10^{-10}$.Comment: 39 pages, 5 figures, v2: references added, minor corrections, v3: matches version published in JCA

Multiplex RT-PCR Amplification of HIV Genes to Create a Completely Autologous DC-Based Immunotherapy for the Treatment of HIV Infection

BACKGROUND: Effective therapy for HIV-infected individuals remains an unmet medical need. Promising clinical trials with dendritic cell (DC)-based immunotherapy consisting of autologous DC loaded with autologous virus have been reported, however, these approaches depend on large numbers of HIV virions to generate sufficient doses for even limited treatment regimens. METHODOLOGY/PRINCIPAL FINDINGS: The present study describes a novel approach for RT-PCR amplification of HIV antigens. Previously, RT-PCR amplification of autologous viral sequences has been confounded by the high mutation rate of the virus which results in unreliable primer-template binding. To resolve this problem we developed a multiplex RT-PCR strategy that allows reliable strain-independent amplification of highly polymorphic target antigens from any patient and requires neither viral sequence data nor custom-designed PCR primers for each individual. We demonstrate the application of our RT-PCR process to amplify translationally-competent RNA encoding regions of Gag, Vpr, Rev and Nef. The products amplified using this method represent a complex mixture of autologous antigens encoded by viral quasispecies. We further demonstrate that DCs electroporated with in vitro-transcribed HIV RNAs are capable of stimulating poly-antigen-specific CD8+ T cell responses in vitro. CONCLUSION/SIGNIFICANCE: This study describes a strategy to overcome patient to patient viral diversity enabling strain-independent RT-PCR amplification of RNAs encoding sequence divergent quasispecies of Gag, Vpr, Rev and Nef from small volumes of infectious plasma. The approach allows creation of a completely autologous therapy that does not require advance knowledge of the HIV genomic sequences, does not have yield limitations and has no intact virus in the final product. The simultaneous use of autologous viral antigens and DCs may provoke broad patient-specific immune responses that could potentially induce effective control of viral loads in the absence of conventional antiretroviral drug therapy

In the social factory? Immaterial labour, precariousness and cultural work

This article introduces a special section concerned with precariousness and cultural work. Its aim is to bring into dialogue three bodies of ideas -- the work of the autonomous Marxist 'Italian laboratory'; activist writings about precariousness and precarity; and the emerging empirical scholarship concerned with the distinctive features of cultural work, at a moment when artists, designers and (new) media workers have taken centre stage as a supposed 'creative class' of model entrepreneurs. The paper is divided into three sections. It starts by introducing the ideas of the autonomous Marxist tradition, highlighting arguments about the autonomy of labour, informational capitalism and the 'factory without walls', as well as key concepts such as multitude and immaterial labour. The impact of these ideas and of Operaismo politics more generally on the precarity movement is then considered in the second section, discussing some of the issues that have animated debate both within and outside this movement, which has often treated cultural workers as exemplifying the experiences of a new 'precariat'. In the third and final section of the paper we turn to the empirical literature about cultural work, pointing to its main features before bringing it into debate with the ideas already discussed. Several points of overlap and critique are elaborated -- focusing in particular on issues of affect, temporality, subjectivity and solidarity

Effectiveness of preoperative beta-blockade on intra-operative heart rate in vascular surgery cases conducted under regional or local anesthesia

BACKGROUND: Preoperative β-blockade has been posited to result in better outcomes for vascular surgery patients by attenuating acute hemodynamic changes associated with stress. However, the incremental effectiveness, if any, of β-blocker usage in blunting heart rate responsiveness for vascular surgery patients who avoid general anesthesia remains unknown. METHODS: We reviewed an existing database and identified 213 consecutive vascular surgery cases from 2005–2011 conducted without general anesthesia (i.e., under monitored anesthesia care or regional anesthesia) at a tertiary care Veterans Administration medical center and categorized patients based on presence or absence of preoperative β-blocker prescription. For this series of patients, with the primary outcome of maximum heart rate during the interval between operating room entry to surgical incision, we examined the association of maximal heart rate and preoperative β-blocker usage by performing crude and multivariate linear regression, adjusting for relevant patient factors. RESULTS: Of 213 eligible cases, 137 were prescribed preoperative β-blockers, and 76 were not. The two groups were comparable across baseline patient factors and intraoperative medication doses. The β-blocker group experienced lower maximal heart rates during the period of evaluation compared to the non-β-blocker group (85 ± 22 bpm vs. 98 ± 36 bpm, respectively; p = 0.002). Adjusted linear regression confirmed a statistically-significant association between lower maximal heart rate and the use of β-blockers (Beta = -11.5; 95% CI [-3.7, -19.3] p = 0.004). CONCLUSIONS: The addition of preoperative β-blockers, even when general anesthesia is avoided, may be beneficial in further attenuating stress-induced hemodynamic changes for vascular surgery patients

Factors affecting the prevalence of strongly and weakly carcinogenic and lower-risk human papillomaviruses in anal specimens in a cohort of men who have sex with men (MSM)

Background: MSM are at higher risk for invasive anal cancer. Twelve human papillomaviruses (HPVs) cause cervical cancer in women (Group 1 high-risk HPVs (hrHPVs)) and 13 HPVs are probable/possible causes (Group 2 hrHPVs) of cervical malignancy. HPVs rarely associated with malignancy are classified as lower-risk HPVs (lrHPVs). Materials and Methods: Dacron-swab anal-cytology specimens were collected from and data complete for 97% (1262/1296) of Multicenter AIDS Cohort Study (MACS) men tested for HPVs using the Linear Array assay. Multivariate Poisson regression analyses estimated adjusted prevalence ratios for Group 1/2 hrHPVs and lrHPVs, controlling for the effects of age, race, ethnicity, sexual partnerships, smoking; HIV-infection characteristics, treatment, and immune status among HIV-infected men. Results: HIV-infected men showed 35-90% higher prevalence of Group 1/2 hrHPVs and lrHPVs than HIV-uninfected men, and higher prevalence of multi-Type, and multiple risk-group infections. CD4+ T-cell count was inversely associated with HPV Group 2 prevalence (p<0.0001). The number of receptive anal intercourse (RAI) partners reported in the 24 months preceding HPV testing predicted higher prevalence of Group 1/2 hrHPVs. Men reporting ≥30 lifetime male sex partners before their first MACS visit and men reporting ≥1 RAI partners during the 24 months before HPV testing showed 17-24% and 13-17% higher prevalence of lrHPVs (p-values ≤0.05). Men reporting smoking between MACS visit 1 and 24 months before HPV testing showed 1.2-fold higher prevalence of Group 2 hrHPVs (p = 0.03). Both complete adherence to CART (p = 0.02) and HIV load <50 copies/mL (p = 0.04) were protective for Group 1 hrHPVs among HIV-infected men. Conclusions: HIV-infected men more often show multi-type and multi-group HPV infections HIV-uninfected men. Long-term mutual monogamy and smoking cessation, generally, and CART-adherence that promotes (HIV) viremia control and prevents immunosuppression, specifically among HIV-infected MSM, are important prevention strategies for HPV infections that are relevant to anal cancer. © 2013 Wiley et al