FRONTAL PLANE KNEE DISPLACEMENT IN BARBELL BACK SQUAT

The aim of the study was to analyze the ML knee displacement and its influence on barbell tilt and rotation displacement from the back squat. Based on the weight distribution (WtD) test, 13 were in the equal WtD group and 14 were in the unequal WtD group. All subjects performed 75% of 1RM barbell back squat with reflective markers on selected locations for motion analysis. Medial-lateral (ML) knee displacement, barbell tilt and rotation displacements were considered. The results showed a statistically significant difference between groups (

The development of microbicides: A new method of HIV prevention for women

A critical review of current epidemiological trends and social science research demonstrates that there is an urgent need for expanding the range of female-controlled HIV prevention methods. Existing efforts to control the spread of HIV infection primarily through the encouragement of a reduction in the number of sexual partners, widespread condom promotion, and the control of other sexually transmitted infections are inadequate for many of the world\u27s women. Underlying gender power inequities severely limit the ability of many women to protect themselves from HIV infection, especially in the absence of a prevention technology they can use, when necessary, without their partner\u27s consent. Current understanding of biology suggests that developing such methods is a feasible and potentially cost-effective endeavor. This paper describes the growing risk of HIV infection faced by women throughout the world, examines the limitation of contemporary AIDS prevention strategy in meeting the needs of women, reviews the existing data on female-controlled HIV prevention methods, and outlines the challenges for future microbicide development

Heterogeneity of cell surface glutamate and GABA receptor expression in Shank and CNTN4 autism mouse models

Autism spectrum disorder (ASD) refers to a large set of neurodevelopmental disorders, which have in common both repetitive behavior and abnormalities in social interactions and communication. Interestingly, most forms of ASD have a strong genetic contribution. However, the molecular underpinnings of this disorder remain elusive. The SHANK3 gene (and to a lesser degree SHANK2) which encode for the postsynaptic density (PSD) proteins SHANK3/SHANK2 and the CONTACTIN 4 gene which encodes for the neuronal glycoprotein CONTACTIN4 (CNTN4) exhibit mutated variants which are associated with ASD. Like many of the other genes associated with ASD, both SHANKs and CNTN4 affect synapse formation and function and are therefore related to the proper development and signaling capability of excitatory and inhibitory neuronal networks in the adult mammal brain. In this study we used mutant/knock-out mice of Shank2 (Shank2-/-), Shank3 (Shank3αβ-/-), and Cntn4 (Cntn4-/-) as ASD-models to explore whether these mice share a molecular signature in glutamatergic and GABAergic synaptic transmission in ASD-related brain regions. Using a biotinylation assay and subsequent western blotting we focused our analysis on cell surface expression of classical several ionotropic glutamate and GABA receptor subunits: GluA1, GluA2, and NR1GluN1 were analyzed for excitatory synaptic transmission, and the α1 subunit of the GABAA receptor was analyzed for inhibitory synaptic transmission. We found that both Shank2-/- and Shank3αβ-/- mice exhibit reduced levels of several cell surface glutamate receptors in most of the analyzed brain regions – especially in the striatum and thalamus – when compared to wildtype controls. Interestingly, even though Cntn4-/- mice also show reduced levels of some cell surface glutamate receptors in the cortex and hippocampus, increased levels of cell surface glutamate receptors were found in the striatum. Moreover, Cntn4-/- mice do not only show brain region-specific alterations in cell surface glutamate receptors but also a downregulation of cell surface GABA receptors in several of the analyzed brain regions. The results of this study suggest that even though mutations in defined genes can be associated with ASD this does not necessarily result in a common molecular phenotype in surface expression of glutamatergic and GABAergic receptor subunits in defined brain regions

Optimizing the construction of outcome measures for impact evaluations of intimate partner violence prevention interventions

Most impact evaluations of intimate partner violence (IPV) prevention interventions use binary measures of "any" versus "no" physical and/or sexual IPV as their primary outcome measure, missing opportunities to capture nuance. In this study, we reanalyzed secondary data from six randomized controlled trials conducted in low- and middle-income countries-Bandebereho (Rwanda), Becoming One (Uganda), Indashyikirwa (Rwanda), MAISHA CRT01, MAISHA CRT02 (Tanzania), Stepping Stones Creating Futures (South Africa), and Unite for a Better Life (Ethiopia), to assess how different conceptualizations and coding of IPV variables can influence interpretations of the impact of an intervention. We compared the standard outcome measures to new measures that reflect the severity and intensity of violence and whether interventions prevent new cases of IPV or reduce or stop ongoing violence. Results indicate that traditional binary indicators masked some of the more subtle intervention effects, and the use of the new indicators allowed for a better understanding of the impacts of the interventions. Conclusions on whether a program is perceived "to work" are highly influenced by the IPV outcomes that the investigators choose to report, and how they are measured and coded. Lack of attention to outcome choice and measurement could lead to prematurely abandoning strategies useful for violence reduction or missing essential insights into how programs may or may not affect IPV. While these results must be interpreted cautiously, given differences in intervention types, the underlying prevalence of violence, sociodemographic factors, sample sizes, and other contextual differences across the trial sites, they can help us move toward a new approach to reporting multiple outcomes that allow us to unpack the "impact" of an intervention by assessing intervention effect by the severity of violence and type of prevention, whether primary and secondary

ORFEUS II Far-UV Spectroscopy of AM Herculis

Six high-resolution (\lambda/\Delta\lambda ~ 3000) far-UV (\lambda\lambda = 910-1210 \AA) spectra of the magnetic cataclysmic variable AM Herculis were acquired in 1996 November during the flight of the ORFEUS-SPAS II mission. AM Her was in a high optical state at the time of the observations, and the spectra reveal emission lines of O VI \lambda\lambda 1032, 1038, C III \lambda 977, \lambda 1176, and He II \lambda 1085 superposed on a nearly flat continuum. Continuum flux variations can be described as per Gansicke et al. by a ~ 20 kK white dwarf with a ~ 37 kK hot spot covering a fraction f~0.15 of the surface of the white dwarf, but we caution that the expected Lyman absorption lines are not detected. The O VI emission lines have narrow and broad component structure similar to that of the optical emission lines, with radial velocities consistent with an origin in the irradiated face of the secondary and the accretion funnel, respectively. The density of the narrow- and broad-line regions is n_{nlr} ~ 3\times 10^{10} cm^{-3} and n_{blr} ~ 1\times 10^{12} cm^{-3}, respectively, yet the narrow-line region is optically thick in the O VI line and the broad-line region is optically thin; apparently, the velocity shear in the broad-line region allows the O VI photons to escape, rendering the gas effectively optically thin. Unexplained are the orbital phase variations of the emission-line fluxes.Comment: 15 pages, 6 Postscript figures; LaTeX format, uses aaspp4.sty; table2.tex included separately because it must be printed sideways - see instructions in the file; accepted on April 17, 1998 for publication in The Astrophysical Journa

Tamoxifen inhibits the biosynthesis of inositolphosphorylceramide in Leishmania

Previous work from our group showed that tamoxifen, an oral drug that has been in use for the treatment of breast cancer for over 40 years, is active both in vitro and in vivo against several species of Leishmania, the etiological agent of leishmaniasis. Using a combination of metabolic labeling with [3H]-sphingosine and myo-[3H]-inositol, alkaline hydrolysis, HPTLC fractionations and mass spectrometry analyses, we observed a perturbation in the metabolism of inositolphosphorylceramides (IPCs) and phosphatidylinositols (PIs) after treatment of L. amazonensis promastigotes with tamoxifen, with a significant reduction in the biosynthesis of the major IPCs (composed of d16:1/18:0-IPC, t16:0/C18:0-IPC, d18:1/18:0-IPC and t16:0/20:0-IPC) and PIs (sn-1-O-(C18:0)alkyl -2-O-(C18:1)acylglycerol-3-HPO4-inositol and sn-1-O-(C18:0)acyl-2-O-(C18:1)acylglycerol-3-HPO4-inositol) species. Substrate saturation kinetics of myo-inositol uptake analyses indicated that inhibition of inositol transport or availability were not the main reasons for the reduced biosynthesis of IPC and PI observed in tamoxifen treated parasites. An in vitro enzymatic assay was used to show that tamoxifen was able to inhibit the Leishmania IPC synthase with an IC50 value of 8.48 μM (95% CI 7.68–9.37), suggesting that this enzyme is most likely one of the targets for this compound in the parasites

Identifying Needs of Potential Transfer Students of Color to Enhance Equity and Inclusion in the College of Health Professions

State Council of Higher Education of Virginia (SCHEV)1 data indicate disparities in recruitment and retention outcomes for transfer students of color and those of Hispanic ethnicity compared to non-Hispanic White peers. With the goal of enhancing equity and inclusion while diversifying the health professions, this project characterizes the needs of this population to inform the development of a new bachelor’s program within the College of Health Professions. The project provides recommendations based on data collected from both prospective and current VCU transfer students