Constraints on the binary black hole hypothesis for system LB-1

At about 70 solar masses, the recently-discovered dark object orbited by a B-type star in the system LB-1 is difficult to understand as the end point of standard stellar evolution, except as a binary black hole (BBH). LB-1 shows a strong, broad H-alpha emission line that is best attributed to a gaseous disk surrounding the dark mass. We use the observed H-alpha line shape, particularly its wing extension, to constrain the inner radius of the disk and thereby the separation of a putative BBH. The hypothesis of a current BBH is effectively ruled out on the grounds that its merger time must be a small fraction of the current age of the B star. The hypothesis of a previous BBH that merged to create the current dark mass is also effectively ruled out by the low orbital eccentricity, due to the combination of mass loss and kick resulted from gravitational wave emission in any past merger. We conclude that the current dark mass is a single black hole produced by the highly mass-conserving, monolithic collapse of a massive star

Cost-effectiveness of initial stress cardiovascular MR, stress SPECT or stress echocardiography as a gate-keeper test, compared with upfront invasive coronary angiography in the investigation and management of patients with stable chest pain: Mid-term outcomes from the CECaT randomised controlled trial

Objectives: To compare outcomes and cost-effectiveness of various initial imaging strategies in the management of stable chest pain in a long-term prospective randomised trial. Setting: Regional cardiothoracic referral centre in the east of England. Participants: 898 patients (69% man) entered the study with 869 alive at 2 years of follow-up. Patients were included if they presented for assessment of stable chest pain with a positive exercise test and no prior history of ischaemic heart disease. Exclusion criteria were recent infarction, unstable symptoms or any contraindication to stress MRI. Primary outcome measures: The primary outcomes of this follow-up study were survival up to a minimum of 2 years post-treatment, quality-adjusted survival and cost-utility of each strategy. Results: 898 patients were randomised. Compared with angiography, mortality was marginally higher in the groups randomised to cardiac MR (HR 2.6, 95% CI 1.1 to 6.2), but similar in the single photon emission CT-methoxyisobutylisonitrile (SPECT-MIBI; HR 1.0, 95% CI 0.4 to 2.9) and ECHO groups (HR 1.6, 95% CI 0.6 to 4.0). Although SPECT-MIBI was marginally superior to other non-invasive tests there were no other significant differences between the groups in mortality, quality-adjusted survival or costs. Conclusions: Non-invasive cardiac imaging can be used safely as the initial diagnostic test to diagnose coronary artery disease without adverse effects on patient outcomes or increased costs, relative to angiography. These results should be interpreted in the context of recent advances in imaging technology. Trial registration: ISRCTN 47108462, UKCRN 3696

Independent evaluation of a simple clinical prediction rule to identify right ventricular dysfunction in patients with shortness of breath

BACKGROUND: Many patients have unexplained persistent dyspnea after negative computed tomographic pulmonary angiography (CTPA). We hypothesized that many of these patients have isolated right ventricular (RV) dysfunction from treatable causes. We previously derived a clinical decision rule (CDR) for predicting RV dysfunction consisting of persistent dyspnea and normal CTPA, finding that 53% of CDR-positive patients had isolated RV dysfunction. Our goal is to validate this previously derived CDR by measuring the prevalence of RV dysfunction and outcomes in dyspneic emergency department patients. METHODS: A secondary analysis of a prospective observational multicenter study that enrolled patients presenting with suspected PE was performed. We included patients with persistent dyspnea, a nonsignificant CTPA, and formal echo performed. Right ventricular dysfunction was defined as RV hypokinesis and/or dilation with or without moderate to severe tricuspid regurgitation. RESULTS: A total of 7940 patients were enrolled. Two thousand six hundred sixteen patients were analyzed after excluding patients without persistent dyspnea and those with a significant finding on CTPA. One hundred ninety eight patients had echocardiography performed as standard care. Of those, 19% (95% confidence interval [CI], 14%-25%) and 33% (95% CI, 25%-42%) exhibited RV dysfunction and isolated RV dysfunction, respectively. Patients with isolated RV dysfunction or overload were more likely than those without RV dysfunction to have a return visit to the emergency department within 45 days for the same complaint (39% vs 18%; 95% CI of the difference, 4%-38%). CONCLUSION: This simple clinical prediction rule predicted a 33% prevalence of isolated RV dysfunction or overload. Patients with isolated RV dysfunction had higher recidivism rates and a trend toward worse outcomes

Kinematic classifications of local interacting galaxies: implications for the merger/disk classifications at high-z

The classification of galaxy mergers and isolated disks is key for understanding the relative importance of galaxy interactions and secular evolution during the assembly of galaxies. The kinematic properties of galaxies as traced by emission lines have been used to suggest the existence of a significant population of high-z star-forming galaxies consistent with isolated rotating disks. However, recent studies have cautioned that post-coalescence mergers may also display disk-like kinematics. To further investigate the robustness of merger/disk classifications based on kinematic properties, we carry out a systematic classification of 24 local (U)LIRGs spanning a range of galaxy morphologies: from isolated spiral galaxies, ongoing interacting systems, to fully merged remnants. We artificially redshift the WiFeS observations of these local (U)LIRGs to z=1.5 to make a realistic comparison with observations at high-z, and also to ensure that all galaxies have the same spatial sampling of ~900 pc. Using both kinemetry-based and visual classifications, we find that the reliability of kinematic classification shows a strong trend with the interaction stage of galaxies. Mergers with two nuclei and tidal tails have the most distinct kinematic properties compared to isolated disks, whereas a significant population of the interacting disks and merger remnants are indistinguishable from isolated disks. The high fraction of late-stage mergers showing disk-like kinematics reflects the complexity of the dynamics during galaxy interactions. However, the exact fractions of misidentified disks and mergers depend on the definition of kinematic asymmetries and the classification threshold when using kinemetry-based classifications. Our results suggest that additional indicators such as morphologies traced by stars or molecular gas are required to further constrain the merger/disk classifications at high-z.Comment: 16 pages, 5 figures, ApJ accepte

Constructing a Man-Made Oyster Reef in the Little Hellgate Salt Marsh, Randall\u27s Island, New York: A Project Overview

Atlantic oyster beds provide instrumental estuarine infrastructure, shaping waterways and mediating the impacts of storm surge. In addition, the reefs provide habitat for numerous species. The oysters themselves are expert filter feeders, remediating poor water quality at little cost to the organism. As the free-swimming oyster larvae (spat) only settle on the shells of their forbears, the reefs are essential in perpetuation of the species, as well as for the continuation of such invaluable ecosystem services. Oyster reefs of the New York City waterway system have long been subject to environmental degradation, from water pollution and sedimentation to dredging. Randall’s Island Park Alliance’s Natural Areas crew received a donation of six hundred oysters from the New York Harbor School to conduct a test for the viability of a manmade oyster garden in 2014. Three hundred oysters were placed in each cage, which were then established in different parts of Randall’s Island Park’s Harlem River shoreline. One was hung by the 103rd Street footbridge seawall and the other was placed on the seawall by the Little Hellgate Salt Marsh inlet. The cages were monitored monthly for oyster growth and mortality rate, and water quality was tested as well. After a year of monitoring, it was determined that the Little Hellgate Salt Marsh was the ideal site for a man-made oyster reef—oysters had shown significantly more growth and a lower mortality rate than those in the cage at the 103rd St. Bridge, most likely due to the protective shape of the salt marsh inlet. The cages that will serve as the reef framework were constructed in the spring of this year and await the placement of oyster shells, which are being treated and sterilized (completion is projected to be late fall 2015). Future monitoring of oyster colonization and growth are expected, with the aim that water quality will measurably improve

Intestinal Microbiome Richness of Coral Reef Damselfishes (Actinopterygii: Pomacentridae)

Fish gastro-intestinal system harbors diverse microbiomes that affect the host's digestion, nutrition, and immunity. Despite the great taxonomic diversity of fish, little is understood about fish microbiome and the factors that determine its structure and composition. Damselfish are important coral reef species that play pivotal roles in determining algae and coral population structures of reefs. Broadly, damselfish belong to either of two trophic guilds based on whether they are planktivorous or algae-farming. In this study, we used 16S rRNA gene sequencing to investigate the intestinal microbiome of 5 planktivorous and 5 algae-farming damselfish species (Pomacentridae) from the Great Barrier Reef. We detected Gammaproteobacteria ASVs belonging to the genus Actinobacillus in 80% of sampled individuals across the 2 trophic guilds, thus, bacteria in this genus can be considered possible core members of pomacentrid microbiomes. Algae-farming damselfish had greater bacterial alpha-diversity, a more diverse core microbiome and shared 35 ± 22 ASVs, whereas planktivorous species shared 7 ± 3 ASVs. Our data also highlight differences in microbiomes associated with both trophic guilds. For instance, algae-farming damselfish were enriched in Pasteurellaceae, whilst planktivorous damselfish in Vibrionaceae. Finally, we show shifts in bacterial community composition along the intestines. ASVs associated with the classes Bacteroidia, Clostridia, and Mollicutes bacteria were predominant in the anterior intestinal regions while Gammaproteobacteria abundance was higher in the stomach. Our results suggest that the richness of the intestinal bacterial communities of damselfish reflects host species diet and trophic guild

Utilization of alkyne bioconjugations to modulate protein function

The ability to introduce or modify protein function has widespread application to multiple scientific disciplines. The introduction of unique unnatural amino acids represents an excellent mechanism to incorporate new functionality; however, this approach is limited by ability of the translational machinery to recognize and incorporate the chemical moiety. To overcome this potential limitation, we aimed to exploit the functionality of existing unnatural amino acids to perform bioorthogonal reactions to introduce the desired protein modification, altering its function. Specifically, via the introduction of a terminal alkyne containing unnatural amino acid, we demonstrated chemically programmable protein modification through the Glaser-Hay coupling to other terminal alkynes, altering the function of a protein. In a proof-of-concept experiment, this approach has been utilized to modify the fluorescence spectrum of green fluorescent protein. (C) 2016 Elsevier Ltd. All rights reserved

Microaxial Support After Orthotopic Heart Transplantation

Introduction and Objective Impella is a temporary ventricular support device intended for short term use (CP, and ≤ 6 days for Impella 5.0 and LD). There has yet to be a study investigating the outcomes of microaxial support for patients who previously underwent heart transplantation. We aimed to assess utilization patterns of microaxial mechanical circulatory support after heart transplantation in adults and adolescents.https://jdc.jefferson.edu/surgeryposters/1012/thumbnail.jp

Distractors slow information accumulation in simple feature search

presented a speed-accuracy trade-off experiment, investigating covert attention in visual search. One of the conclusions from Carrasco and McElree was that adding distractors to a single feature search does not decrease the speed with which information is accumulated about target identity. We present a reanalysis of the relevant data from Carrasco and McElree in which we demonstrate that their conclusion was incomplete and we demonstrate a processing speed advantage for single feature search displays with no distractors compared with displays with distractors. This finding is confirmed in a new speed-accuracy trade-off experiment presented here. Further, we demonstrate that increasing the display duration increases the processing speed of displays with distractors but not for displays without distractors. We discuss these results in relation to theories of visual attention and the debate between graded and fixed architecture accounts for attentional allocation