Evaluation verhaltenspräventiver Gesundheitsförderungsmaßnahmen in Inklusionsbetrieben

Hintergrund: Inklusionsbetriebe nach §§ 215-218 SGB IX bieten schwerbehinderten Menschen eine Beschäftigung auf dem allgemeinen Arbeitsmarkt. Seit 2018 sind Inklusionsbetriebe verpflichtet, Maßnahmen der betrieblichen Gesundheitsförderung (BGF) anzubieten. Ziel der Arbeit: Das Ziel der vorliegenden Studie ist es, verhaltenspräventive BGF-Angebote in Form von Seminaren für schwerbehinderte Beschäftigte und deren Leitungskräfte in Inklusionsbetrieben zu evaluieren. Material und Methoden: Anhand eines multimethodischen Studiendesigns erfolgte im Zeitraum von Juni bis November 2021 eine Evaluation der 12 Beschäftigtenseminare (3 Seminarkonzepte, z. B. Selbstfürsorge im Arbeitsalltag) mittels Fokusgruppen (n = 44) und eine Evaluation der 3 Leitungskräfteseminare zur gesunden Führung mittels eines standardisierten Fragebogens (n = 10). Die erhobenen quantitativen Daten wurden deskriptiv ausgewertet und die qualitativen Daten mithilfe der qualitativen Inhaltsanalyse nach Mayring induktiv analysiert. Ergebnisse: Die Ergebnisse zeigten, dass die durchgeführten Maßnahmen zur BGF von Beschäftigten hinsichtlich der Zufriedenheit, der Seminarlänge, der Verständlichkeit und des erwarteten Nutzens mehrheitlich positiv bewertet wurden. Die Leitungskräfte bewerteten die Seminarinhalte, die Didaktik, die Seminarleitung und den Beitrag der Teilnehmenden mehrheitlich positiv. Diskussion: Die Studie lieferte erste empirische Erkenntnisse zur Evaluation von BGF-Maßnahmen in Inklusionsbetrieben. Vor allem die auf die Inklusionsbetriebe angepassten Seminarinhalte konnten die Zufriedenheit der Teilnehmenden mit der Gesundheitsförderungsmaßnahme positiv beeinflussen. Insgesamt bedarf es weiterer Studien zur Entwicklung und Evaluation von verhältnis- und verhaltensbezogenen BGF-Maßnahmen in Inklusionsbetrieben.Background: Social firms according to §§ 215-218 SGB IX offer employment to severely disabled people on the general labour market. Since 2018, social firms are obliged to offer workplace health promotion (WHP) measures. Objectives: This study aims to evaluate behavioural-related offers implemented as seminars for WHP for severely disabled employees and their supervisors in social firms. Materials and methods: Using a multimethod study design, from June to November 2021, 12 seminars for employees (3 seminar concepts, e.g. on self-care in everyday work) were evaluated by means of focus groups (n = 44) and 3 seminars for supervisors on healthy leadership by means of a standardized questionnaire (n = 10). The collected quantitative data were analysed descriptively and the qualitative data were analysed inductively according to Mayring’s qualitative content analysis. Results: The results indicated that the implemented WHP measures for employees were rated positively by the majority in terms of satisfaction, seminar length, comprehensibility and usefulness. Similarly, supervisors evaluated the seminar content, didactics, instructor as well as the contribution of other participants mostly positively. Conclusion: The study provided initial empirical findings on the evaluation of WHP measures in social firms. In particular, seminar content adapted to the setting of social firms had a positive influence on participants’ satisfaction with health promotion measures. Overall, further studies are needed to develop and evaluate structural and behavioural-related WHP offers in social firms

SARS-CoV-2 Omicron variant is attenuated for replication in a polarized human lung epithelial cell model

SARS-CoV-2 and its emerging variants of concern remain a major threat for global health. Here we introduce an infection model based upon polarized human Alveolar Epithelial Lentivirus immortalized (hAELVi) cells grown at the air–liquid interface to estimate replication and epidemic potential of respiratory viruses in the human lower respiratory tract. hAELVI cultures are highly permissive for different human coronaviruses and seasonal influenza A virus and upregulate various mediators following virus infection. Our analysis revealed a significantly reduced capacity of SARS-CoV-2 Omicron BA.1 and BA.2 variants to propagate in this human model compared to earlier D614G and Delta variants, which extends early risk assessments from epidemiological and animal studies suggesting a reduced pathogenicity of Omicron.Peer Reviewe

SARS-CoV-2 variant Alpha has a spike-dependent replication advantage over the ancestral B.1 strain in human cells with low ACE2 expression

Epidemiological data demonstrate that Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) variants of concern (VOCs) Alpha and Delta are more transmissible, infectious, and pathogenic than previous variants. Phenotypic properties of VOC remain understudied. Here, we provide an extensive functional study of VOC Alpha replication and cell entry phenotypes assisted by reverse genetics, mutational mapping of spike in lentiviral pseudotypes, viral and cellular gene expression studies, and infectivity stability assays in an enhanced range of cell and epithelial culture models. In almost all models, VOC Alpha spread less or equally efficiently as ancestral (B.1) SARS-CoV-2. B.1. and VOC Alpha shared similar susceptibility to serum neutralization. Despite increased relative abundance of specific sgRNAs in the context of VOC Alpha infection, immune gene expression in infected cells did not differ between VOC Alpha and B.1. However, inferior spreading and entry efficiencies of VOC Alpha corresponded to lower abundance of proteolytically cleaved spike products presumably linked to the T716I mutation. In addition, we identified a bronchial cell line, NCI-H1299, which supported 24-fold increased growth of VOC Alpha and is to our knowledge the only cell line to recapitulate the fitness advantage of VOC Alpha compared to B.1. Interestingly, also VOC Delta showed a strong (595-fold) fitness advantage over B.1 in these cells. Comparative analysis of chimeric viruses expressing VOC Alpha spike in the backbone of B.1, and vice versa, showed that the specific replication phenotype of VOC Alpha in NCI-H1299 cells is largely determined by its spike protein. Despite undetectable ACE2 protein expression in NCI-H1299 cells, CRISPR/Cas9 knock-out and antibody-mediated blocking experiments revealed that multicycle spread of B.1 and VOC Alpha required ACE2 expression. Interestingly, entry of VOC Alpha, as opposed to B.1 virions, was largely unaffected by treatment with exogenous trypsin or saliva prior to infection, suggesting enhanced resistance of VOC Alpha spike to premature proteolytic cleavage in the extracellular environment of the human respiratory tract. This property may result in delayed degradation of VOC Alpha particle infectivity in conditions typical of mucosal fluids of the upper respiratory tract that may be recapitulated in NCI-H1299 cells closer than in highly ACE2-expressing cell lines and models. Our study highlights the importance of cell model evaluation and comparison for in-depth characterization of virus variant-specific phenotypes and uncovers a fine-tuned interrelationship between VOC Alpha- and host cell-specific determinants that may underlie the increased and prolonged virus shedding detected in patients infected with VOC Alpha

SARS-CoV-2 variant Alpha has a spike-dependent replication advantage over the ancestral B.1 strain in human cells with low ACE2 expression

Epidemiological data demonstrate that Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) variants of concern (VOCs) Alpha and Delta are more transmissible, infectious, and pathogenic than previous variants. Phenotypic properties of VOC remain understudied. Here, we provide an extensive functional study of VOC Alpha replication and cell entry phenotypes assisted by reverse genetics, mutational mapping of spike in lentiviral pseudotypes, viral and cellular gene expression studies, and infectivity stability assays in an enhanced range of cell and epithelial culture models. In almost all models, VOC Alpha spread less or equally efficiently as ancestral (B.1) SARS-CoV-2. B.1. and VOC Alpha shared similar susceptibility to serum neutralization. Despite increased relative abundance of specific sgRNAs in the context of VOC Alpha infection, immune gene expression in infected cells did not differ between VOC Alpha and B.1. However, inferior spreading and entry efficiencies of VOC Alpha corresponded to lower abundance of proteolytically cleaved spike products presumably linked to the T716I mutation. In addition, we identified a bronchial cell line, NCI-H1299, which supported 24-fold increased growth of VOC Alpha and is to our knowledge the only cell line to recapitulate the fitness advantage of VOC Alpha compared to B.1. Interestingly, also VOC Delta showed a strong (595-fold) fitness advantage over B.1 in these cells. Comparative analysis of chimeric viruses expressing VOC Alpha spike in the backbone of B.1, and vice versa, showed that the specific replication phenotype of VOC Alpha in NCI-H1299 cells is largely determined by its spike protein. Despite undetectable ACE2 protein expression in NCI-H1299 cells, CRISPR/Cas9 knock-out and antibody-mediated blocking experiments revealed that multicycle spread of B.1 and VOC Alpha required ACE2 expression. Interestingly, entry of VOC Alpha, as opposed to B.1 virions, was largely unaffected by treatment with exogenous trypsin or saliva prior to infection, suggesting enhanced resistance of VOC Alpha spike to premature proteolytic cleavage in the extracellular environment of the human respiratory tract. This property may result in delayed degradation of VOC Alpha particle infectivity in conditions typical of mucosal fluids of the upper respiratory tract that may be recapitulated in NCI-H1299 cells closer than in highly ACE2-expressing cell lines and models. Our study highlights the importance of cell model evaluation and comparison for in-depth characterization of virus variant-specific phenotypes and uncovers a fine-tuned interrelationship between VOC Alpha- and host cell-specific determinants that may underlie the increased and prolonged virus shedding detected in patients infected with VOC Alpha.Peer Reviewe

Boundarylessness and sleep quality among virtual team members – a pilot study from Germany

Background!#!In the course of globalisation and digitalisation, new ways of work are becoming increasingly prevalent. To remain competitive as an organisation, cooperation across time, place, and organisational boundaries is becoming necessary. Virtual teamwork offers these advantages, but can also be both, an opportunity and a burden, for employees. This pilot study aims to gain first insights into job demands and resources in virtual teamwork to provide a basis for further research from which appropriate health promotion and prevention measures can be derived.!##!Methods!#!In this pilot study, an online questionnaire was used to examine the relationship between boundarylessness as a job demand, psychological detachment as a personal resource, as well as perceived stress and sleep quality as health outcomes among 46 virtual team members from Germany. Data collection lasted from October 2019 to January 2020. Validated scales were used for the questionnaire, except for virtuality. Due to insufficient operationalisation to date, a virtuality scale was developed based on the current state of research. The data were analysed with ordinal logistic regression analyses and median split !##!Results!#!The results indicate that perceived stress impaired sleep quality of virtual team members in this sample. In contrast, successful psychological detachment from work was positively related to sleep quality. A higher degree of virtuality coincided with higher levels of boundarylessness. Virtual team members with leadership responsibility showed higher levels of psychological detachment.!##!Conclusion!#!The present pilot study breaks ground and provides initial insights into the relationship between virtual teamwork and employee health in the German context. Further research, particularly on job demands in virtual teamwork, is needed to derive concrete health promotion and prevention measures

Arbeitsbezogene Gesundheit in Inklusionsbetrieben – eine Übersicht zur Arbeits- und Gesundheitssituation der Beschäftigten und der betrieblichen Gesundheitsförderung

<jats:title>Zusammenfassung</jats:title><jats:sec> <jats:title>Hintergrund</jats:title> <jats:p>Inklusionsbetriebe bieten Menschen mit geistigen oder körperlichen Einschränkungen einen geschützten Beschäftigungsrahmen auf dem allgemeinen Arbeitsmarkt. Aufgrund der gesundheitlichen Notwendigkeit bei der Zielgruppe Menschen mit Behinderung und der kürzlich reformierten gesetzlichen Vorgaben nimmt die betriebliche Gesundheitsförderung einen wichtigen Stellenwert in Inklusionsbetrieben ein.</jats:p> </jats:sec><jats:sec> <jats:title>Fragestellung</jats:title> <jats:p>In dem Artikel wird eine Übersicht zum Forschungsstand zur Arbeits- und Gesundheitssituation von Beschäftigten in Inklusionsbetrieben sowie die aktuelle Umsetzung von Gesundheitsförderungsmaßnahmen präsentiert.</jats:p> </jats:sec><jats:sec> <jats:title>Ergebnisse</jats:title> <jats:p>Forschungsergebnisse deuten darauf hin, dass Erwerbsarbeit durch ihre vielen potenziell gesundheitsförderlichen und sozial-inklusiven Effekte für Beschäftige mit Behinderung viele Ressourcen (z. B. soziale Unterstützung oder Flexibilität) mit sich bringt.</jats:p> </jats:sec><jats:sec> <jats:title>Schlussfolgerung</jats:title> <jats:p>Forschungsbedarf wird v. a. hinsichtlich evidenzbasierter Interventionsmaßnahmen und in der Analyse gesundheitlicher Belastungen der in Inklusionsbetrieben beschäftigten Menschen mit und ohne Behinderung sichtbar. Zur Entwicklung gesundheitsförderlicher Arbeitsbedingungen werden in diesem Beitrag Empfehlungen auf Basis der bisherigen Forschung, v. a. für die Kategorien Arbeitsinhalte, Arbeitsorganisation und soziale Beziehungen zusammengefasst.</jats:p> </jats:sec&gt

Working Conditions in Social Firms and Health Promotion Interventions in Relation to Employees’ Health and Work-Related Outcomes—A Scoping Review

Background: Social firms—a type of social enterprise—offer people with severe disabilities the possibility of employment and integration into the labor market. Since 01 January 2018, social firms in Germany are obligated to provide health promotion interventions for their employees. Therefore, the study aims to provide an overview of the current state of research on working conditions, coping strategies, work- and health-related outcomes, and health promotion interventions in social firms to derive recommendations for action. Methods: The databases PubMed, MEDLINE, PsycINFO, PSYNDEX, CINAHL, and Web of Science were searched. The study selection was based on predefined inclusion and exclusion criteria in the time period between 2000 and 2019. The quality of the studies was critically appraised in a standardized way using the Mixed-Methods Appraisal Tool. Results: A total of 25 studies were included. The current state of research indicated that employees with disabilities were provided with several environmental resources like social support, flexibility, structured work tasks or options for training. A mix of environmental and personal resources impacted several work- and health-related outcomes like well-being, job satisfaction, productivity, work engagement, the motivation to work, or job tenure. Conclusions: There is a need for further (longitudinal) research concerning the work and health situation of employees working in social firms and the development of health promotion interventions

Workplace Health Promotion in German Social Firms—Offers, Needs and Challenges from the Perspectives of Employees, Supervisors and Experts

On the general labor market, social firms provide 30–50% of people with different types of disabilities the opportunity to gain employment. However, the topic of workplace health promotion (WHP), needs for improvement and accompanied challenges are neglected in the current research and were the focus of the present study. Therefore, data triangulation was used between July and December 2020 by combining three focus groups with employees (n = 14 employees) with 16 interviews with supervisors from several social firms in Northern Germany (e.g., from catering, cleaning or bicycle repair sectors). 17 semi-structured telephone interviews with experts in the field of WHP or social firms were added. All approaches were audio-taped, transcribed and anonymized. To analyze the data, Mayring’s qualitative content analysis was used. The results indicated that several offers for WHP, including sport, nutrition and relaxation, were offered, as well as those on smoking cessation, cooperation with external organizations or training and education offers. Needs for improvement were stated referring to additional sport offers, support for implementing a healthy diet, offers for relaxation, financial incentives or collaborations with external organizations. A low take-up of offers; a lack of resources, structures or management support; compatibility of offers with work time and organization; challenges with available trainings or the consideration of individual needs and capacities were highlighted as challenges. Overall, there is a need for further interventional and longitudinal research on WHP in social firms

Balancing social and economic factors : explorative qualitative analysis of working conditions of supervisors in German social firms

Social firms are companies on the general labour market which provide employment to people with severe disabilities. In this setting different job resources are offered for its employees, including social support or flexibility in terms of working hours, tasks or pace of work. However, to date, only limited evidence exists on the work and health situation of supervisors in social firms. Therefore, the study aims to explore job demands and resources of supervisors in social firms to increase knowledge in a little researched field and to develop recommendations for action on workplace health promotion

Functional comparison of MERS-coronavirus lineages reveals increased replicative fitness of the recombinant lineage 5.

Middle East respiratory syndrome coronavirus (MERS-CoV) is enzootic in dromedary camels across the Middle East and Africa. Virus-induced pneumonia in humans results from animal contact, with a potential for limited onward transmission. Phenotypic changes have been suspected after a novel recombinant clade (lineage 5) caused large nosocomial outbreaks in Saudi Arabia and South Korea in 2016. However, there has been no functional assessment. Here we perform a comprehensive in vitro and ex vivo comparison of viruses from parental and recombinant virus lineages (lineage 3, n = 7; lineage 4, n = 8; lineage 5, n = 9 viruses) from Saudi Arabia, isolated immediately before and after the shift toward lineage 5. Replication of lineage 5 viruses is significantly increased. Transcriptional profiling finds reduced induction of immune genes IFNB1, CCL5, and IFNL1 in lung cells infected with lineage 5 strains. Phenotypic differences may be determined by IFN antagonism based on experiments using IFN receptor knock out and signaling inhibition. Additionally, lineage 5 is more resilient against IFN pre-treatment of Calu-3 cells (ca. 10-fold difference in replication). This phenotypic change associated with lineage 5 has remained undiscovered by viral sequence surveillance, but may be a relevant indicator of pandemic potential