Impacto da Pandemia Causada pelo SARS-COV-2 nos Depósitos de Patente da Fiocruz

Covid-19 has promoted deep changes in the organization of society and in the scientific and technological areas. Publications and knowledge-generating interactions resulted in an unprecedented number of scientific articles, patents, products, and services that were made available on the market very fast. By means of patentometrics on Orbit Intelligence® (Questel), Espacenet, and INPI databases, patent documents owned by Fiocruz were retrieved and analyzed, in order to assess the impact of the pandemic on the number and profile of patents filed by the institution. The results indicate a potential contribution of about 11.5% of patents originated from efforts and responses to the health emergency caused by the new coronavirus in relation to the total number of Fiocruz patents, further emphasizing the institution's role in facing the pandemic and the legacy to Brazilian Society.A Covid-19 provocou profundas mudanças na organização da sociedade e nas áreas científica e tecnológica. Publicações e interações geradoras de conhecimento resultaram em uma quantidade inédita de artigos científicos, patentes, produtos e serviços disponibilizados rapidamente no mercado. Por meio da prospecção patentária nas bases de dados Orbit Intelligence® (Questel), Espacenet e do INPI, foram recuperados e analisados documentos de patente sob propriedade da Fiocruz, com o objetivo de avaliar o impacto da pandemia no número e perfil de patentes depositadas pela instituição. Os resultados indicam potencial contribuição de cerca de 11,5% de patentes originadas a partir dos esforços e respostas à emergência sanitária provocada pelo novo coronavírus em relação ao total de patentes da Fiocruz, concretizando ainda mais o protagonismo da instituição no enfrentamento da pandemia e o legado para a sociedade brasileira

FONTES DE INFORMAÇÃO TECNOLÓGICA EM BIOTECNOLOGIA: VARIEDADE, CONFIABILIDADE E USO POR SISTEMAS DE INFORMAÇÃO, ORGANIZAÇÕES E GRUPOS DE PESQUISA

Com sua importância amplamente reconhecida, a informação tecnológica tem sido cada vez mais utilizada por indivíduos e organizações na busca pela competitividade e sustentabilidade das atividades produtivas. Em função disso, a demanda pela informação tecnológica qualificada e objetiva como ferramenta técnica e gerencial tem aumentado consideravelmente nos últimos anos e vem sendo discutida por diversos autores. No presente trabalho buscou-se identificar e apresentar diversas fontes de informação tecnológica disponíveis e relevantes em Biotecnologia, discutir sua tipologia, confiabilidade e alcance, consubstanciar lacunas, bem como reforçar a importância dos serviços de informação no suporte às atividades voltadas à inovação tecnológica neste campo

Flow Cytometry as a Tool for Quality Control of Fluorescent Conjugates Used in Immunoassays

Submitted by Sandra Infurna ([email protected]) on 2017-03-28T15:46:20Z No. of bitstreams: 1 alvaro_bertho_etal_IOC_2016.pdf: 2901056 bytes, checksum: 7d5255b1b013920484bc8b0918988719 (MD5)Approved for entry into archive by Sandra Infurna ([email protected]) on 2017-03-28T16:01:38Z (GMT) No. of bitstreams: 1 alvaro_bertho_etal_IOC_2016.pdf: 2901056 bytes, checksum: 7d5255b1b013920484bc8b0918988719 (MD5)Made available in DSpace on 2017-03-28T16:01:38Z (GMT). No. of bitstreams: 1 alvaro_bertho_etal_IOC_2016.pdf: 2901056 bytes, checksum: 7d5255b1b013920484bc8b0918988719 (MD5) Previous issue date: 2016Fundação Oswaldo Cruz. Instituto de Tecnologia Imunobiológica - Farmanguinhos. Laboratório de Tecnologia de Diagnóstico. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia Imunobiológica - Farmanguinhos. Laboratório de Tecnologia de Diagnóstico. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia Imunobiológica - Farmanguinhos. Laboratório de Tecnologia de Diagnóstico. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia Imunobiológica - Farmanguinhos. Laboratório de Tecnologia de Diagnóstico. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Clínica. Rio de Janeiro, RJ. Brasil.The use of antibodies in immunodiagnostic kits generally implies the conjugation of these proteins with other molecules such as chromophores or fluorochromes. The development of more sensitive quality control procedures than spectrophotometry is essential to assure the use of better fluorescent conjugates since the fluorescent conjugates are critical reagents for a variety of immunodiagnostic kits. In this article, we demonstrate a new flow cytometric protocol to evaluate conjugates by molecules of equivalent soluble fluorochromes (MESF) and by traditional flow cytometric analysis. We have coupled microspheres with anti-IgG-PE and anti-HBSAg-PE conjugates from distinct manufactures and/or different lots and evaluated by flow cytometry. Their fluorescence intensities were followed for a period of 18 months. Our results showed that there was a great difference in the fluorescence intensities between the conjugates studied. The differences were observed between manufactures and lots from both anti-IgG-PE and anti-HBSAg-PE conjugates. Coefficients of variation (CVs) showed that this parameter can be used to determine better coupling conditions, such as homogenous coupling. The MESF analysis, as well as geometric mean evaluation by traditional flow cytometry, showed a decrease in the values for all conjugates during the study and were indispensable tools to validate the results of stability tests. Our data demonstrated the feasibility of the flow cytometric method as a standard quality control of immunoassay kits

Conjugate stability study during 18 months.

<p>A: A1, A2, A3, A4 and MESF bead #4 fluorescence intensities during the months of analysis. B: B1, B2, C, D and MESF bead #4 fluorescence intensities during the months of analysis. C: Conjugates histograms of PE-fluorescence intensity (X axis) vs. count (Y axis) on the first month (black peak) and last month of analysis (white peak). MESF bead #4 was included as an example of the MESF kit used to control instrument performance during the 18 months of evaluation. The A4 conjugate was coupled later than the other conjugates and its intensities evaluation started at month 4.</p

Staining profile of anti-IgG-PE conjugates.

<p>A: Representative histograms of PE-fluorescence intensity (X axis) vs. count (Y axis). 0.5 μg/mL = white peak and 5 μg/mL = black peak. B: Coefficient of variation of 0.5 μg/mL (white bars) and 5 μg/mL (black bars). Data expressed as the means of CVs from of three tests ± standard deviation. T test was performed for group comparison. An asterisk indicates a significantly different value assumed at p≤0.05.</p

FITC and PE detection after anti-IgG-FITC staining.

<p>FITC and PE detection after anti-IgG-FITC staining.</p

Evaluation of the best conjugate concentration and dilution.

<p>A: Anti-IgG-PE concentration test (0.5, 1, 2.5 and 5 μg/mL). * = statistically different value when compared to the lowest concentration (p≤0.05). A1- black bars, A4- dashed bars and B2- white bars. B: Anti-HBSAg-PE dilution test (1:3000, 1:1000 and 1:300). C- black bars and D- dashed bars. One way ANOVA was performed for group comparison * = statistically different value among concentrations (p≤0.05). Data are expressed as mean of three geometric means ± standard deviation.</p

Evaluation of different codes of microspheres (#012, #035 and #070).

<p>A: Microspheres (#012, #035 and #070) coupled with 5 μg/mL of A1 (black bars) and A4 (dashed bars) conjugates. Fluorescence intensity represented by the means of three geometric means ± standard deviation. B: Representative histograms of one experiment. PE-fluorescence intensity (X axis) vs. count (Y axis). One way ANOVA was performed for group comparison and significant differences were observed among all groups (p≤0.05).</p