81 research outputs found
Origin and evolution of the RIG-I like RNA helicase gene family
Peer reviewedPublisher PD
Individual monitoring of immune response in Atlantic salmon Salmo salar following experimental infection with Infectious Salmon Anaemia Virus (ISAV)
This work was funded by the National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs, grant G1100675). The authors are grateful to the Marine Scotland aquarium staff (Ben Williamson, Louise Feehan and Mark Paterson) for help in the experimental work. Hendrix-Genetix (formerly Landcatch Natural Selection) is also acknowledged for providing PIT tagged fish for the purpose of this project.Peer reviewedPublisher PD
A putative serine protease, SpSsp1, from Saprolegnia parasitica is recognised by sera of rainbow trout, Oncorhynchus mykiss
Acknowledgements Our work was supported by the BBSRC (BB/C518457/1, BB/G012075/1, BB/J018333/1) (K.L.M., C.J.S., J.S.C., K.S.D., and P.v.W.), the University of Aberdeen (V.L.A., C.J.S., and P.v.W.), MSD Animal Health (J.S.C., K.S.D., and A.H.v.d.B), and The Royal Society (P.v.W.). This work was also supported by a Marie Curie Initial Training Networks with the SAPRO (sustainable approaches to reduce Oomycete (Saprolegnia) infections in aquacultures) grant PITN-GA-2009-238550 (A.H.v.d.B., L.L., C.J.S., P.v.W.). We would like to acknowledge Aberdeen Proteomics for carrying out LC–MS/MS and Laura Grenville-Briggs for valuable discussion and technical help. We are grateful to the Broad Institute (Carsten Russ, Rays Jiang, Brian Haas, and Chad Nusbaum), Brett Tyler (VBI), and P.v.W. for early release of draft supercontigs of the genome sequence of isolate CBS233.65, which helped us identify SpSsp1.Peer reviewedPublisher PD
Sequence and Expression Analysis of Interferon Regulatory Factor 10 (IRF10) in Three Diverse Teleost Fish Reveals Its Role in Antiviral Defense
Acknowledgments This research was supported financially by the National Natural Science Foundation of China (31101928), the National Science and Technology Support Program of China (2013BAD20B06), the State Key Laboratory of Freshwater Ecology and Biotechnology (2010FB02) and the MASTS pooling initiative (The Marine Alliance for Science and Technology for Scotland) funded by the Scottish Funding Council (grant reference HR09011). Q.X. and Y.J. were supported financially by the National Scholarship Council of China. Funding: This research was supported financially by the National Natural Science Foundation of China (31101928), the National Science and Technology Support Program of China (2013BAD20B06), the State Key Laboratory of Freshwater Ecology and Biotechnology (2010FB02) and the MASTS pooling initiative (The Marine Alliance for Science and Technology for Scotland) funded by the Scottish Funding Council (grant reference HR09011). Q.X. and Y.J. were supported financially by the National Scholarship Council of China. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Peer reviewedPublisher PD
Sequence and expression analysis of rainbow trout CXCR2, CXCR3a and CXCR3b aids interpretation of lineage-specific conversion, loss and expansion of these receptors during vertebrate evolution
Copyright © 2014 The Authors. Published by Elsevier Ltd.. All rights reserved. Open Access funded by Biotechnology and Biological Sciences Research Council This work received funding from the MASTS pooling initiative (The Marine Alliance for Science and Technology for Scotland). MASTS is funded by the Scottish Funding Council (grant reference HR09011) and contributing institutions. Q.X. and Y.J. were supported financially by the National Scholarship Council of China, J.W.H by the Biotechnology and Biological Sciences Research Council (BB/K009125/1), and M.M.M. by European Commision LIFECYCLE project (222919).Peer reviewedPublisher PD
CD4 homologue in sea bass (Dicentrarchus labrax): molecular characterization and structural analysis
CD4 is a transmembrane glycoprotein fundamental for cell-mediated immunity. Its action as a T cell coreceptor
increases the avidity of association between a T cell and an antigen-presenting cell by interacting
with portions of the complex between MHC class II and TR molecules. In this paper we report the cDNA
cloning, expression and structural analysis of a CD4 homologue from sea bass (Dicentrarchus labrax). The
sea bass CD4 cDNA consists of 2071 bp that translates in one reading frame to give the entire molecule
containing 480 amino acids. The analysis of the sequence shows the presence of four putative Ig-like
domains and that some fundamental structural features, like a disulphide bond in domain D2 and the CXC
signalling motif in the cytoplasmic tail, are conserved from sea bass to mammals. Real-time PCR analysis
showed that very high levels of CD4 mRNA transcripts are present in thymus, followed by gut and gills. In
vitro stimulation of head kidney leukocytes with LPS and PHA-L gave an increase of CD4 mRNA levels after
4 h and a decrease after 24 h. Homology modelling has been applied to create a 3D model of sea bass CD4
and to investigate its interaction with sea bass MHC-II. The analysis of the 3D complex between sea bass
CD4 and sea bass MHC-II suggests that the absence of a disulfide bond in the CD4 D1 domain could make
this molecule more flexible, inducing a different conformation and affecting the binding and the way of
interaction between CD4 and MHC-II. Our results will add new insights into the sea bass T cell immune
responses and will help in the identification of T cell subsets in teleost fishes to better understand the
evolution of cell-mediated immunity from fish to mammals.L'articolo è disponibile sul sito dell'editore http://www.sciencedirect.com
Characterisation of the TNF superfamily members CD40L and BAFF in the small-spotted catshark (Scyliorhinus canicula)
The tumour necrosis factor superfamily (TNFSF) members CD40L and BAFF play critical roles in mammalian B cell survival, proliferation and maturation, however little is known about these key cytokines in the oldest jawed vertebrates, the cartilaginous fishes. Here we report the cloning of CD40L and BAFF orthologues (designated ScCD40L and ScBAFF) in the small-spotted catshark (Scyliorhinus canicula). As predicted both proteins are type II membrane-bound proteins with a TNF homology domain in their extracellular region and both are highly expressed in shark immune tissues. ScCD40L transcript levels correlate with those of TCRα and transcription of both genes is modulated in peripheral blood leukocytes following in vitro stimulation. Although a putative CD40L orthologue was identified in the elephant shark genome the work herein is the first molecular characterisation and transcriptional analysis of CD40L in a cartilaginous fish. ScBAFF was also cloned and its transcription characterised in an attempt to resolve the discrepancies observed between spiny dogfish BAFF and bamboo shark BAFF in previously published studies
Distinct Differentiation Programs Triggered by IL-6 And LPS in Teleost IgM+ B Cells in the Absence of Germinal Centers
We would like to thank Lucia Gonzalez and Maria Sanz for technical assistance. Professor Øystein Evensen is also acknowledged for providing us with the inactivated IPNV. This work was supported by the European Research Council (ERC Starting Grant 2011 280469) and by the European Commission under the 7th Framework Programme for Research and Technological Development (FP7) of the European Union (Grant Agreement 311993 TARGETFISH). T. W. received funding from the MASTS pooling initiative (The Marine Alliance for Science and Technology for Scotland). MASTS is funded by the Scottish Funding Council (grant reference HR09011).Peer reviewedPublisher PD
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