Generalized self-driven AC-DC synchronous rectification techniques for single- and multiphase systems

This paper extends the single-phase self-driven synchronous rectification (SDSR) technique to multiphase ac-dc systems. Power MOSFETs with either voltage- or current-sensing self-driven gate drives are used to replace the diodes in the rectifier circuits. The generalized methodology allows multiphase SDSRs to be designed to replace the multiphase diode rectifiers. Unlike the traditional SR that is designed for high-frequency power converters, the SDSR proposed here can be a direct replacement of the power diode bridges for both low- and high-frequency operations. The SDSR utilizes its output dc voltage to supply power to its control circuit. No start-up control is needed because the body diodes of the power MOSFETs provide the diode rectifier for the initial start-up stage. The generalized method is demonstrated in 2-kW one-phase and three-phase SDSRs for inductive, capacitive, and resistive loads. Power loss reduction in the range of 50%-69% has been achieved for the resistive load. © 2009 IEEE.published_or_final_versio

Fiber-coupled light-emitting diode with a capillary-bonded hemispherical lens

A hemispherical lens capillary-bonded to an InGaN flip-chip light-emitting diode (LED) is demonstrated to efficiently couple light to a plastic optical fiber. The BK-7 hemispherical lens is bonded onto a circularly shaped LED chip with inclined sidewalls cut by laser-micromachining, so that lateral emissions are effectively suppressed. Capillary bonding minimizes air-gap between chip and lens enabling transmission of evanescent waves, thus maximizing overall optical transmission. With the lens attached, emission divergence from the assembly is significantly reduced, diverting rays into the acceptance cone of the fiber. Fiber coupling efficiency as high as 53.8% has been demonstrated. © 2011 IEEE.published_or_final_versio

Structural And Functional Prediction Of Hypothetical Proteins From Klebsiella Pneumoniae MGH78578: Molecular Modelling Studies

Twenty percent of the genes from Klebsiella pneumonaie MGH78578 coded for hypothetical protein. Two particular hypothetical proteins KPN00728 and KPN00729 were identified using bioinformatics approaches. Both open reading frames showed high sequence homology to succinate dehydrogenase Chain C (SdhC) and D (SdhD) from Escherichia coli KPN00729 was annotated as SdhD in May 2008. Thus, investigation on KPN00728 remained as no annotation for SdhC gene in the complete genome sequence of Klebsiella pneumoniae MGH78578. In this study, KPN00728 has a missing region with conserved residues which is important for ubiquinone (UQ) and heme group binding. Structure and function prediction of KPN00728 coupled with secondary structure analysis and transmembrane topology showed KPN00728 adopts SDH-(subunit C)-like structure

A pancake-shaped nano-aggregate for focusing surface plasmons

We proposed a pancake-shaped nano-aggregate that highly focuses surface plasmons. The structure is a superposition of bowtie-shaped dimers, where surface plasmons are excited, resonated with the structure, and coupled. Surface integral equation method (Poggio-Miller-Chang-Harrington-Wu-Tsai method) is used to predict the performance of the proposed structure. It is a method which can accurately calculate the near-fields of nanoparticles. Based on the numerical prediction, the proposed structure shows an electric field (E-field) enhancement of more than 400 times, which is equivalent to a Raman enhancement factor of more than 2.5 e 10 times. It is promising for single molecule detections using surface-enhanced Raman scattering. The physics of the proposed structure are revealed. It is useful to design nanostructures for high E-field enhancement. © 2012 American Institute of Physics.published_or_final_versio

Potential impact of reactive vaccination in controlling cholera outbreaks: An exploratory analysis using a Zimbabwean experience

Background. To contain ongoing cholera outbreaks, the World Health Organization has suggested that reactive vaccination should be considered in addition to its previous control measures. Objectives. To explore the potential impact of a hypothetical reactive oral cholera vaccination using the example of the recent large-scale cholera outbreak in Zimbabwe. Methods. This was a retrospective cost-effectiveness analysis calculating the health and economic burden of the cholera outbreak in Zimbabwe with and without reactive vaccination. The primary outcome measure was incremental cost per disability-adjusted life year (DALY) averted. Results. Under the base-case assumptions (assuming 50% coverage among individuals aged ≥2 years), reactive vaccination could have averted 1 320 deaths and 23 650 DALYs. Considering herd immunity, the corresponding values would have been 2 920 deaths and 52 360 DALYs averted. The total vaccination costs would have been ~$74 million and ~$21 million, respectively, with per-dose vaccine price of US$5 and$1. The incremental costs per DALY averted of reactive vaccination were $2 770 and$370, respectively, for vaccine price set at $5 and$1. Assuming herd immunity, the corresponding cost was $980 with vaccine price of$5, and the programme was cost-saving with a vaccine price of $1. Results were most sensitive to case-fatality rate, per-dose vaccine price, and the size of the outbreak. Conclusions. Reactive vaccination has the potential to be a costeffective measure to contain cholera outbreaks in countries at high risk. However, the feasibility of implementation should be further evaluated, and caution is warranted in extrapolating the findings to different settings in the absence of other in-depth studies

TRPCs: Influential Mediators in Skeletal Muscle

Ca2+ itself or Ca2+-dependent signaling pathways play fundamental roles in various cellular processes from cell growth to death. The most representative example can be found in skeletal muscle cells where a well-timed and adequate supply of Ca2+ is required for coordinated Ca2+-dependent skeletal muscle functions, such as the interactions of contractile proteins during contraction. Intracellular Ca2+ movements between the cytosol and sarcoplasmic reticulum (SR) are strictly regulated to maintain the appropriate Ca2+ supply in skeletal muscle cells. Added to intracellular Ca2+ movements, the contribution of extracellular Ca2+ entry to skeletal muscle functions and its significance have been continuously studied since the early 1990s. Here, studies on the roles of channel proteins that mediate extracellular Ca2+ entry into skeletal muscle cells using skeletal myoblasts, myotubes, fibers, tissue, or skeletal muscle-originated cell lines are reviewed with special attention to the proposed functions of transient receptor potential canonical proteins (TRPCs) as store-operated Ca2+ entry (SOCE) channels under normal conditions and the potential abnormal properties of TRPCs in muscle diseases such as Duchenne muscular dystrophy (DMD)

Controlled release of human growth hormone fused with a human hybrid Fc fragment through a nanoporous polymer membrane

Nanotechnology has been applied to the development of more effective and compatible drug delivery systems for therapeutic proteins. Human growth hormone (hGH) was fused with a hybrid Fc fragment containing partial Fc domains of human IgD and IgG(4) to produce a long-acting fusion protein. The fusion protein, hGH-hyFc, resulted in the increase of the hydrodynamic diameter (ca. 11 nm) compared with the diameter (ca. 5 nm) of the recombinant hGH. A diblock copolymer membrane with nanopores (average diameter of 14.3 nm) exhibited a constant release rate of hGH-hyFc. The hGH-hyFc protein released in a controlled manner for one month was found to trigger the phosphorylation of Janus kinase 2 (JAK2) in human B lymphocyte and to exhibit an almost identical circular dichroism spectrum to that of the original hGH-hyFc, suggesting that the released fusion protein should maintain the functional and structural integrity of hGH. Thus, the nanoporous release device could be a potential delivery system for the long-term controlled release of therapeutic proteins fused with the hybrid Fc fragment.X111313sciescopu

Higgs Physics at the Large Hadron Collider

In this talk I will begin by summarising the importance of the Higgs physics studies at the LHC. I will then give a short description of the pre-LHC constraints on the Higgs mass and the theoretical predictions for the LHC along with a discussion of the current experimental results, ending with prospects in the near future at the LHC. In addition to the material covered in the presented talk, I have included in the writeup, a critical appraisal of the theoretical uncertainties in the Higgs cross-sections at the Tevatron as well as a discussion of the recent experimental results from the LHC which have become available since the time of the workshop.Comment: LateX, 12 figures, 15 pages, Presented at the XIth Workshop on High Energy Physics Phenomenology, 2010, Ahmedabad, Indi

Bioluminescence-Activated Deep-Tissue Photodynamic Therapy of Cancer

Optical energy can trigger a variety of photochemical processes useful for therapies. Owing to the shallow penetration of light in tissues, however, the clinical applications of light-activated therapies have been limited. Bioluminescence resonant energy transfer (BRET) may provide a new way of inducing photochemical activation. Here, we show that efficient bioluminescence energy-induced photodynamic therapy (PDT) of macroscopic tumors and metastases in deep tissue. For monolayer cell culture in vitro incubated with Chlorin e6, BRET energy of about 1 nJ per cell generated as strong cytotoxicity as red laser light irradiation at 2.2 mW/cm(2) for 180 s. Regional delivery of bioluminescence agents via draining lymphatic vessels killed tumor cells spread to the sentinel and secondary lymph nodes, reduced distant metastases in the lung and improved animal survival. Our results show the promising potential of novel bioluminescence-activated PDT.111813Ysciescopu