247 research outputs found
Golgi Outpost Synthesis Impaired by Toxic Polyglutamine Proteins Contributes to Dendritic Pathology in Neurons
Dendrite aberration is a common feature of neurodegenerative diseases caused by protein toxicity, but the underlying mechanisms remain largely elusive. Here, we show that nuclear polyglutamine (polyQ) toxicity resulted in defective terminal dendrite elongation accompanied by a loss of Golgi outposts (GOPs) and a decreased supply of plasma membrane (PM) in Drosophila class IV dendritic arborization (da) (C4 da) neurons. mRNA sequencing revealed that genes downregulated by polyQ proteins included many secretory pathway-related genes, including COPII genes regulating GOP synthesis. Transcription factor enrichment analysis identified CREB3L1/CrebA, which regulates COPII gene expression. CrebA overexpression in C4 da neurons restores the dysregulation of COPII genes, GOP synthesis, and PM supply. Chromatin immunoprecipitation (ChIP)-PCR revealed that CrebA expression is regulated by CREB-binding protein (CBP), which is sequestered by polyQ proteins. Furthermore, co-overexpression of CrebA and Rac1 synergistically restores the polyQ-induced dendrite pathology. Collectively, our results suggest that GOPs impaired by polyQ proteins contribute to dendrite pathology through the CBP-CrebA-COPII pathway. ? 2017 The Author(s)113Ysciescopu
Probabilistic reasoning with a bayesian DNA device based on strand displacement
We present a computing model based on the DNA strand displacement technique which performs Bayesian inference. The model will take single stranded DNA as input data, representing the presence or absence of a specific molecular signal (evidence). The program logic encodes the prior probability of a disease and the conditional probability of a signal given the disease playing with a set of different DNA complexes and their ratios. When the input and program molecules interact, they release a different pair of single stranded DNA species whose relative proportion represents the application of Bayes? Law: the conditional probability of the disease given the signal. The models presented in this paper can empower the application of probabilistic reasoning in genetic diagnosis in vitro
Search for and Using Genetic Programming Event Selection
We apply a genetic programming technique to search for the double Cabibbo
suppressed decays and .
We normalize these decays to their Cabibbo favored partners and find
\Lambda_c^+ \to p K^+ \pi^-\Lambda_c^+ \to p K^-
\pi^+ and D_s^+ \to K^+ K^+
\pi^-D_s^+ \to K^+ K^- \pi^+ where
the first errors are statistical and the second are systematic. Expressed as
90% confidence levels (CL), we find and respectively.
This is the first successful use of genetic programming in a high energy
physics data analysis.Comment: 10 page
Measurement of the D+ and Ds+ decays into K+K-K+
We present the first clear observation of the doubly Cabibbo suppressed decay
D+ --> K-K+K+ and the first observation of the singly Cabibbo suppressed decay
Ds+ --> K-K+K+. These signals have been obtained by analyzing the high
statistics sample of photoproduced charm particles of the FOCUS(E831)
experiment at Fermilab. We measure the following relative branching ratios:
Gamma(D+ --> K-K+K+)/Gamma(D+ --> K-pi+pi+) = (9.49 +/- 2.17(statistical) +/-
0.22(systematic))x10^-4 and Gamma(Ds+ --> K-K+K+)/Gamma(Ds+ --> K-K+pi+) =
(8.95 +/- 2.12(statistical) +2.24(syst.) -2.31(syst.))x10^-3.Comment: 10 pages, 8 figure
A Non-parametric Approach to the D+ to K*0bar mu+ nu Form Factors
Using a large sample of D+ -> K- pi+ mu+ nu decays collected by the FOCUS
photoproduction experiment at Fermilab, we present the first measurements of
the helicity basis form factors free from the assumption of spectroscopic pole
dominance. We also present the first information on the form factor that
controls the s-wave interference discussed in a previous paper by the FOCUS
collaboration. We find reasonable agreement with the usual assumption of
spectroscopic pole dominance and measured form factor ratios.Comment: 14 pages, 5 figures, and 2 tables. We updated the previous version by
changing some words, removing one plot, and adding two tables. These changes
are mostly stylisti
Measurements of Branching Ratios
Using data collected by the fixed target Fermilab experiment FOCUS, we
measure the branching ratios of the Cabibbo favored decays , , and relative to to be
, , and ,
respectively. We report the first observation of the Cabibbo suppressed decay
and we measure the branching ratio relative to
to be . We also set 90%
confidence level upper limits for and relative to to
be 0.12 and 0.05, respectively. We find an indication of the decays and and set
90% confidence level upper limits for the branching ratios with respect to
to be 0.12 and 1.72, respectively. Finally, we
determine the 90% C.L. upper limit for the resonant contribution relative to to be 0.10.Comment: 14 pages, 8 figure
Dalitz plot analysis of D_s+ and D+ decay to pi+pi-pi+ using the K-matrix formalism
FOCUS results from Dalitz plot analysis of D_s+ and D+ to pi+pi-pi+ are
presented. The K-matrix formalism is applied to charm decays for the first time
to fully exploit the already existing knowledge coming from the light-meson
spectroscopy experiments. In particular all the measured dynamics of the S-wave
pipi scattering, characterized by broad/overlapping resonances and large
non-resonant background, can be properly included. This paper studies the
extent to which the K-matrix approach is able to reproduce the observed Dalitz
plot and thus help us to understand the underlying dynamics. The results are
discussed, along with their possible implications on the controversial nature
of the sigma meson.Comment: To be submitted to Phys.Lett.B A misprint corrected in formula
Measurement of the branching ratio of the decay D^0 -> \pi^-\mu^+\nu relative to D^0 -> K^-\mu^+\nu
We present a new measurement of the branching ratio of the Cabibbo suppressed
decay D^0\to \pi^-\mu^+\nu relative to the Cabibbo favored decay D^0\to
K^-\mu^+\nu and an improved measurement of the ratio
|\frac{f_+^{\pi}(0)}{f_+^{K}(0)}|. Our results are 0.074 \pm 0.008 \pm 0.007
for the branching ratio and 0.85 \pm 0.04 \pm 0.04 \pm 0.01 for the form factor
ratio, respectively.Comment: 13pages, 3 figure
New Measurements of the D+ to K* mu nu Form Factor Ratios
Using a large sample of D+ to K- pi+ mu+ nu decays collected by the FOCUS
photoproduction experiment at Fermilab, we present new measurements of two
semileptonic form factor ratios: rv and r2. We find rv = 1.504 \pm 0.057 \pm
0.039 and r2 = 0.875 \pm 0.049 \pm 0.064. Our form factor results include the
effects of the s-wave interference discussed in a previous paper.Comment: 12 pages, 5 figure
Study of the D^0 \to pi^-pi^+pi^-pi^+ decay
Using data from the FOCUS (E831) experiment at Fermilab, we present new
measurements for the Cabibbo-suppressed decay mode . We measure the branching ratio .
An amplitude analysis has been performed, a first for this channel, in order to
determine the resonant substructure of this decay mode. The dominant component
is the decay , accounting for 60% of the decay rate.
The second most dominant contribution comes from the decay , with a fraction of 25%. We also study the
line shape and resonant substructure. Using the helicity formalism for the
angular distribution of the decay , we measure
a longitudinal polarization of %.Comment: 38 pages, 8 figures. accepted for publication in Physical Review
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