1,235 research outputs found
Cell sorting in a Petri dish controlled by computer vision.
Fluorescence-activated cell sorting (FACS) applying flow
cytometry to separate cells on a molecular basis is a widespread
method. We demonstrate that both fluorescent and unlabeled live
cells in a Petri dish observed with a microscope can be
automatically recognized by computer vision and picked up by a
computer-controlled micropipette. This method can be routinely
applied as a FACS down to the single cell level with a very
high selectivity. Sorting resolution, i.e., the minimum distance
between two cells from which one could be selectively removed
was 50-70 micrometers. Survival rate with a low number of 3T3
mouse fibroblasts and NE-4C neuroectodermal mouse stem cells was
66 +/- 12% and 88 +/- 16%, respectively. Purity of sorted
cultures and rate of survival using NE-4C/NE-GFP-4C co-cultures
were 95 +/- 2% and 62 +/- 7%, respectively. Hydrodynamic
simulations confirmed the experimental sorting efficiency and a
cell damage risk similar to that of normal FACS
Far-infrared surface-plasmon quantum-cascade lasers at 21.5 mu m and 24 mu m wavelengths
Quantum-cascade lasers operating above 20 mum (at lambda similar to 21.5 mum and lambda similar to 24 mum) wavelength are reported. Pulsed operation was obtained up to 140 K and with a peak power of a few milliwatts at cryogenic temperatures. Laser action originates from interminiband transitions in "chirped" superlattice active regions. The waveguides are based on surface-plasmon modes confined at a metal-semiconductor interface. The wavelengths were chosen in order to avoid major phonon absorption bands, which are particularly strong at energies just above the reststrahlen band. We also report on a 21.5-mum-wavelength laser based on a two-sided interface-plasmon waveguide. (C) 2001 American Institute of Physics
Fibronectin-Containing Extracellular Vesicles Protect Melanocytes against Ultraviolet Radiation-Induced Cytotoxicity.
Skin melanocytes are activated by exposure to UV radiation to secrete melanin-containing melanosomes to protect the skin from UV-induced damage. Despite the continuous renewal of the epidermis, the turnover rate of melanocytes is very slow, and they survive for long periods. However, the mechanisms underlying the survival of melanocytes exposed to UV radiation are not known. Here, we investigated the role of melanocyte-derived extracellular vesicles in melanocyte survival. Network analysis of the melanocyte extracellular vesicle proteome identified the extracellular matrix component fibronectin at a central node, and the release of fibronectin-containing extracellular vesicles was increased after exposure of melanocytes to UVB radiation. Using an anti-fibronectin neutralizing antibody and specific inhibitors of extracellular vesicle secretion, we demonstrated that extracellular vesicles enriched in fibronectin were involved in melanocyte survival after UVB radiation. Furthermore, we observed that in the hyperpigmented lesions of patients with melasma, the extracellular space around melanocytes contained more fibronectin compared with normal skin, suggesting that fibronectin is involved in maintaining melanocytes in pathological conditions. Collectively, our findings suggest that melanocytes secrete fibronectin-containing extracellular vesicles to increase their survival after UVB radiation. These data provide important insight into how constantly stimulated melanocytes can be maintained in pathological conditions such as melasma.1166Ysciescopu
Geometry of the Grosse-Wulkenhaar Model
We define a two-dimensional noncommutative space as a limit of finite-matrix
spaces which have space-time dimension three. We show that on such space the
Grosse-Wulkenhaar (renormalizable) action has natural interpretation as the
action for the scalar field coupled to the curvature. We also discuss a natural
generalization to four dimensions.Comment: 16 pages, version accepted in JHE
Membrane-Associated Transporter Protein (MATP) Regulates Melanosomal pH and Influences Tyrosinase Activity
The SLC45A2 gene encodes a Membrane-Associated Transporter Protein (MATP). Mutations of this gene cause oculocutaneous albinism type 4 (OCA4). However, the molecular mechanism of its action in melanogenesis has not been elucidated. Here, we discuss the role of MATP in melanin production. The SLC45A2 gene is highly enriched in human melanocytes and melanoma cell lines, and its protein, MATP, is located in melanosomes. The knockdown of MATP using siRNAs reduced melanin content and tyrosinase activity without any morphological change in melanosomes or the expression of melanogenesis-related proteins. Interestingly, the knockdown of MATP significantly lowered the melanosomal pH, as verified through DAMP analysis, suggesting that MATP regulates melanosomal pH and therefore affects tyrosinase activity. Finally, we found that the reduction of tyrosinase activity associated with the knockdown of MATP was readily recovered by copper treatment in the in vitro L-DOPA oxidase activity assay of tyrosinase. Considering that copper is an important element for tyrosinase activity and that its binding to tyrosinase depends on melanosomal pH, MATP may play an important role in regulating tyrosinase activity via controlling melanosomal pH.112820Ysciescopu
Microfluidic cell sorter with integrated piezoelectric actuator
We demonstrate a low-power (<0.1 mW), low-voltage (<10 Vp-p) on-chip piezoelectrically actuated micro-sorter that can deflect single particles and cells at high-speed. With rhodamine in the stream, switching of flow between channels can be visualized at high actuation frequency (~1.7 kHz). The magnitude of the cell deflection can be precisely controlled by the magnitude and waveform of input voltage. Both simulation and experimental results indicate that the drag force imposed on the suspended particle/cell by the instantaneous fluid displacement can alter the trajectory of the particle/cell of any size, shape, and density of interest in a controlled manner. The open-loop E. Coli cell deflection experiment demonstrates that the sorting mechanism can produce a throughput of at least 330 cells/s, with a promise of a significantly higher throughput for an optimized design. To achieve close-loop sorting operation, fluorescence detection, real-time signal processing, and field-programmable-gate-array (FPGA) implementation of the control algorithms were developed to perform automated sorting of fluorescent beads. The preliminary results show error-free sorting at a sorting efficiency of ~70%. Since the piezoelectric actuator has an intrinsic response time of 0.1–1 ms and the sorting can be performed under high flowrate (particle speed of ~1–10 cm/s), the system can achieve a throughput of >1,000 particles/s with high purity
An Open-System Quantum Simulator with Trapped Ions
The control of quantum systems is of fundamental scientific interest and
promises powerful applications and technologies. Impressive progress has been
achieved in isolating the systems from the environment and coherently
controlling their dynamics, as demonstrated by the creation and manipulation of
entanglement in various physical systems. However, for open quantum systems,
engineering the dynamics of many particles by a controlled coupling to an
environment remains largely unexplored. Here we report the first realization of
a toolbox for simulating an open quantum system with up to five qubits. Using a
quantum computing architecture with trapped ions, we combine multi-qubit gates
with optical pumping to implement coherent operations and dissipative
processes. We illustrate this engineering by the dissipative preparation of
entangled states, the simulation of coherent many-body spin interactions and
the quantum non-demolition measurement of multi-qubit observables. By adding
controlled dissipation to coherent operations, this work offers novel prospects
for open-system quantum simulation and computation.Comment: Pre-review submission to Nature. For an updated and final version see
publication. Manuscript + Supplementary Informatio
Anyonic interferometry and protected memories in atomic spin lattices
Strongly correlated quantum systems can exhibit exotic behavior called
topological order which is characterized by non-local correlations that depend
on the system topology. Such systems can exhibit remarkable phenomena such as
quasi-particles with anyonic statistics and have been proposed as candidates
for naturally fault-tolerant quantum computation. Despite these remarkable
properties, anyons have never been observed in nature directly. Here we
describe how to unambiguously detect and characterize such states in recently
proposed spin lattice realizations using ultra-cold atoms or molecules trapped
in an optical lattice. We propose an experimentally feasible technique to
access non-local degrees of freedom by performing global operations on trapped
spins mediated by an optical cavity mode. We show how to reliably read and
write topologically protected quantum memory using an atomic or photonic qubit.
Furthermore, our technique can be used to probe statistics and dynamics of
anyonic excitations.Comment: 14 pages, 6 figure
Interleukin-17D and Nrf2 mediate initial innate immune cell recruitment and restrict MCMV infection.
Innate immune cells quickly infiltrate the site of pathogen entry and not only stave off infection but also initiate antigen presentation and promote adaptive immunity. The recruitment of innate leukocytes has been well studied in the context of extracellular bacterial and fungal infection but less during viral infections. We have recently shown that the understudied cytokine Interleukin (IL)-17D can mediate neutrophil, natural killer (NK) cell and monocyte infiltration in sterile inflammation and cancer. Herein, we show that early immune cell accumulation at the peritoneal site of infection by mouse cytomegalovirus (MCMV) is mediated by IL-17D. Mice deficient in IL-17D or the transcription factor Nuclear factor (erythroid-derived 2)-like 2 (Nrf2), an inducer of IL-17D, featured an early decreased number of innate immune cells at the point of viral entry and were more susceptible to MCMV infection. Interestingly, we were able to artificially induce innate leukocyte infiltration by applying the Nrf2 activator tert-butylhydroquinone (tBHQ), which rendered mice less susceptible to MCMV infection. Our results implicate the Nrf2/IL-17D axis as a sensor of viral infection and suggest therapeutic benefit in boosting this pathway to promote innate antiviral responses
Spatio-temporal Models of Lymphangiogenesis in Wound Healing
Several studies suggest that one possible cause of impaired wound healing is
failed or insufficient lymphangiogenesis, that is the formation of new
lymphatic capillaries. Although many mathematical models have been developed to
describe the formation of blood capillaries (angiogenesis), very few have been
proposed for the regeneration of the lymphatic network. Lymphangiogenesis is a
markedly different process from angiogenesis, occurring at different times and
in response to different chemical stimuli. Two main hypotheses have been
proposed: 1) lymphatic capillaries sprout from existing interrupted ones at the
edge of the wound in analogy to the blood angiogenesis case; 2) lymphatic
endothelial cells first pool in the wound region following the lymph flow and
then, once sufficiently populated, start to form a network. Here we present two
PDE models describing lymphangiogenesis according to these two different
hypotheses. Further, we include the effect of advection due to interstitial
flow and lymph flow coming from open capillaries. The variables represent
different cell densities and growth factor concentrations, and where possible
the parameters are estimated from biological data. The models are then solved
numerically and the results are compared with the available biological
literature.Comment: 29 pages, 9 Figures, 6 Tables (39 figure files in total
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