Composers' Forum: Student Works, April 10, 1990

This is the concert program of the Composers' Forum: Student Works performance on Tuesday, April 10, 1990 at 12:30 p.m., at the Boston University Concert Hall, 855 Commonwealth Avenue, Boston, Massachusetts. Works performed were ME-A-RI by Nami Cho, Music Box by Juley B. Kurs, Control by Juley B. Kurs, Three Sketches for two pianos by Nami Cho, Preludes for piano by Juley B. Kurs, and Up and Down: Inner Rounds by John Saylor. Digitization for Boston University Concert Programs was supported by the Boston University Humanities Library Endowed Fund

Carbohydrate Mouth Rinse Improves 1.5 h Run Performance: Is There a Dose-Effect?

There is a substantial body of recent evidence showing ergogenic effects of carbohydrate (CHO) mouth rinsing on endurance performance. However, there is a lack of research on the dose-effect and the aim of this study was to investigate the effect of two different concentrations (6% and 12% weight/volume, w/v) on 90 minute treadmill running performance. Seven active males took part in one familiarization trial and three experimental trials (90-minute self-paced performance trials). Solutions (placebo, 6% or 12% CHO-electrolyte solution, CHO-E) were rinsed in the mouth at the beginning, and at 15, 30 and 45 minutes during the run. The total distance covered was greater during the CHO-E trials (6%, 14.6 ± 1.7 km; 12%, 14.9 ± 1.6 km) compared to the placebo trial (13.9 ± 1.7 km, P 0.05). There were no between trial differences (P > 0.05) in ratings of perceived exertion (RPE) and feeling or arousal ratings suggesting that the same subjective ratings were associated with higher speeds in the CHO-E trials. Enhanced performance in the CHO-E trials was due to higher speeds in the last 30 minutes even though rinses were not provided during the final 45 minutes, suggesting the effects persist for at least 20-45 minutes after rinsing. In conclusion, mouth rinsing with a CHO-E solution enhanced endurance running performance but there does not appear to be a dose-response effect with the higher concentration (12%) compared to a standard 6% solution

WSN and RFID integration to support intelligent monitoring in smart buildings using hybrid intelligent decision support systems

The real time monitoring of environment context aware activities is becoming a standard in the service delivery in a wide range of domains (child and elderly care and supervision, logistics, circulation, and other). The safety of people, goods and premises depends on the prompt reaction to potential hazards identified at an early stage to engage appropriate control actions. This requires capturing real time data to process locally at the device level or communicate to backend systems for real time decision making. This research examines the wireless sensor network and radio frequency identification technology integration in smart homes to support advanced safety systems deployed upstream to safety and emergency response. These systems are based on the use of hybrid intelligent decision support systems configured in a multi-distributed architecture enabled by the wireless communication of detection and tracking data to support intelligent real-time monitoring in smart buildings. This paper introduces first the concept of wireless sensor network and radio frequency identification technology integration showing the various options for the task distribution between radio frequency identification and hybrid intelligent decision support systems. This integration is then illustrated in a multi-distributed system architecture to identify motion and control access in a smart building using a room capacity model for occupancy and evacuation, access rights and a navigation map automatically generated by the system. The solution shown in the case study is based on a virtual layout of the smart building which is implemented using the capabilities of the building information model and hybrid intelligent decision support system.The Saudi High Education Ministry and Brunel University (UK

Varietal effects of barley carbohydrate composition on digestibility, fermentability and microbial ecophysiology in an in vitro model of the pig gastrointestinal tract.

Carbohydrate (CHO) composition can vary markedly between barley varieties. Their influence on digestibility, intestinal fermentation and microbiota in pigs was studied in vitro. Ten hulless (HLB) and 6 hulled barleys (HB) differing in B-glucan, non-starch polysaccharides (NSP), starch content, and amylose/amylopectin ratio, were hydrolyzed enzymatically and subsequently fermented for 72h. CHO fermentation kinetics were modeled; microbial composition and short-chain fatty acid (SCFA) production were analyzed. In HLB, in vitro DM digestibility was positively correlated to starch and amylopectin content and CP digestibility to amylopectin (P<0.05), whereas both were negatively correlated to insoluble NSP (P<0.05). Rate of fermentation was different (P<0.01) between barley types but not correlated to the CHO composition. However, high B-glucan contents induced faster fermentation (P<0.05, HLB; P<0.10, HB). SCFA molar ratios after fermentation of HLB were higher in propionate and branchedchain fatty acids and lower in acetate compared to HB (P<0.01). With HLB, amylose content was positively correlated to butyrate production and negatively to propionate, which was positively correlated to soluble NSP content (P<0.01). In HB, no correlation between SCFA production and the carbohydrate composition was found. TRFLP analysis revealed that Bacteroides and members of Clostridium cluster XIVa were differentially affected in HLB compared to HB as well as by the type and source of CHO. Microbial profiles were also correlated (P<0.05) to SCFA and fermentation parameters but response differed significantly between HB and HLB. The strongest correlation between CHO structure, microbial abundance and fermentation parameters was evident in HLB. Hulless barleys may offer the greatest opportunity to improve gut health in pigs

Quarkonium Wave Functions at the Origin

We tabulate values of the radial Schr\"{o}dinger wave function or its first nonvanishing derivative at zero quark-antiquark separation, for $c\bar{c}$, $c\bar{b}$, and $b\bar{b}$ levels that lie below, or just above, flavor threshold. These quantities are essential inputs for evaluating production cross sections for quarkonium states.Comment: 9 pages, RevTeX, no figure

Effect of Oral Rinsing with Pre-Workout on Cycling Time Trial Performance

There is evidence that oral rinsing with a carbohydrate solution can have similar effects on aerobic performance compared to consuming the beverage, but less is known of sugar-free and pre-workout beverages. PURPOSE: to examine the effects of oral rinsing with a pre-workout supplement on aerobic performance. METHODS: After recording age (32.4±12.1 yrs), height (173.2±11.2 cm), and weight (76.8±14.8 kg), eleven participants rinsed 10 sec with either 2.5 oz. of carbohydrate (CHO), sugar-free pre-workout (PWO), or a sugar-free placebo (CN) beverage. Participants were interfaced with a metabolic analyzer and resting measurements were collected after two minutes. Participants then cycled for 5 minutes on a stationary bike as a warm up, with load being adjusted to reach 70% of max heart rate. Participants then rode for 15 minutes at this intensity after which they received a five minute break followed by another 10 second oral rinse of the same solution. This was followed by a time trial, which consisted of 15 minutes on the bike at maximum intensity. This was a counter-balanced, repeated-measures design where participants performed the trials no fewer than two days and no more than seven days apart. Repeated measures ANOVA were used to determine differences in performances and physiological responses between trials, with Alpha set at .05 for all tests. RESULTS: There was no significant difference in distance during the time trial between CHO (7.7±1.2 km), PWO (7.6±1.4 km), and CN (7.4±1.2), F(2,9)=0.95, P=.419. There was also no significant difference in VO2 during the time trial between CHO (2.02±0.58 L/min), PWO (1.94±0.58 L/min), and CN (2.29±1.29 L/min), F(2,9) = 1.0, P=.388, as well as heart rate between CHO (155.6±18.2 b/min), PWO (152.6±19.8 b/min), and CN (150.3±19.1 b/min), F(2,9)=1.5, P=.260. Finally, there was no significant difference in RPE between CHO (14.0±2.6), PWO (13.6±2.3), and CN (13.6±2.1), F(2,9)=.23, P=.797. CONCLUSION: The results of the study suggest that oral rinsing does not enhance performance on a 15-min cycling time trial. For a short, acute bout of intense aerobic exercise, oral rinsing with a CHO or PWO drink will not provide any benefit

Cre-loxP-controlled cell-cycle checkpoint engineering in Chinese Hamster ovary cells

The gene amplification system is widely used in Chinese hamster ovary (CHO) cells for the productive cell line construction of therapeutic proteins. To enhance the efficiency of conventional gene amplification systems, we previously presented a novel method using cell-cycle checkpoint engineering1). Here, we constructed high-producing and stable cells by the conditional expression of mutant cell division cycle 25 homolog B (CDC25B) using the Cre-loxP system2). A bispecific antibody-producing CHO DG44-derived cell line was transfected with floxed mutant CDC25B. After inducing gene amplification in the presence of 250 nM methotrexate, mutant CDC25B sequence was removed by Cre recombinase protein expression. Overexpression of the floxed mutant CDC25B significantly enhanced the efficiency of transgene amplification and productivity. Moreover, the specific production rate of the isolated clone CHO Cre-1 and Cre-2 were approximately 11-fold and 15-fold higher than that of mock-transfected clone CHO Mock-S. Chromosomal aneuploidy was increased by mutant CDC25B overexpression, but Cre-1 and Cre-2 did not show any changes in chromosome number during long-term cultivation, as is the case with CHO Mock-S. Our results suggest that high-producing and stable cells can be constructed by conditionally controlling a cell-cycle checkpoint integrated in conventional gene amplification systems

The effect of carbohydrate dose and timing on timed effort and time to exhaustion within a simulated cycle race in male professional cyclists

A key performance limitation affecting professional endurance cycling is carbohydrate storage and utilisation (Pöchmüller, Schwingshack, Colombani & Hoffmann, 2016, Journal of the International Society of Sports Nutrition, 13). Muscle glycogen stores alone are inefficient at maintaining optimal blood glucose levels beyond two hours of exercise; consequently, exogenous CHO is commonly used to counteract this (Jeukendrup, 2011, Journal of Sports Sciences, 21, 91-99). High concentrations of CHO can cause drops in blood glucose, excessive glycogen utilisation and gastrointestinal discomfort (GID) (Jeukendrup, 2011). Therefore, the aim of this study was to determine if frequent, smaller CHO feedings would be preferable to large, bolus CHO feedings on time trial cycling performance. With institutional ethics approval, 5 professional cyclists completed a 4h simulated cycle ride with 3 timed efforts in a randomised, cross-over, double blind design study. Each timed effort occurred in the last 10 min of each hour (TE1, TE2, TE3); participants were asked to cycle with maximum effort for this time. There was also a final effort at the end of the 4th hour to replicate a sprint finish. This was measured as time to exhaustion (TTE). Two interventions were used; a frequent feed (F) where participants drank 20g maltodextrin in 300ml flavoured water solution 3 times per hour and a bolus feed (B) where participants drank 60g maltodextrin solution once per hour. Heart rate, power output, GID, perceived exertion (RPE), blood lactate and blood glucose were recorded before and after TE1, TE2, TE3 and TTE. Wilcoxen signed rank test and Cohen’s D was performed to study differences between interventions and effect sizes.In the F intervention, average watts were significantly higher at TE2 (P<0.05 d=0.75) and TE3 (P<0.05 d=1.21) and the RPE was lower TE1 (P≥0.05 d=1.12), TE2 (P<0.05, d=1.12) and TTE (P≥0.05 d=1.12) compared to B. There was no significant difference between any other variables. The results suggest that despite power output being higher, RPE was lower in the F intervention. Gut absorption of CHO is limited to 1g/h (Jeukendrup, 2011), which may help explain these findings. This is one of the first studies to look at concentration and timing of CHO consumption in endurance cycling. Regular feeds of 20g CHO may be more beneficial on power output and RPE in endurance cycling compared to hourly 60g feeds