Identification and characterization of bacteria from the skin of jackfruit

Fresh-cut fruits industry is developing extensively in this global era due to busy lifestyle. However, the fresh-cut processing could be contaminated by microorganisms; resulting in food poisoning outbreaks. This study presented about the characterization and inhibition of bacteria isolated from the fresh skin of jackfruit. The isolation of single colonies was done by spread plate and streak plate method. The isolates were identified by 16S rRNA analysis. Gram staining, growth profiling and 15 biochemical tests were carried out to characterize each isolates.Lastly, the isolates were treated with various antimicrobial agents by disc agar diffusion technique and the fresh skin of jackfruit was treated with antimicrobialagent, XY-12 with different conditions by spread plate technique to study the effects of antimicrobial agents on microbial growth. Experimental results demonstrated that four types of bacterial single colonies were isolated with different morphology and designated as CE1, CE2, CE3 and CE4. The 16S rRNA analysis showed that the isolates of CE1 and CE4 were Bacillus sp. strain CY-b33. Isolates of CE2 and CE3were identified as Bacillus pumilus strain SBTBP-008 and Bacillus thuringiensis strain EA26.1 respectively. All the isolates were Gram positive and in rod shaped.The growth kinetics of CE3 was highest (0.2280 h-1) compared to CE1 (0.1317 h-1)which was the lowest. Bacteria CE1 and CE2 have the highest sensitivity againstXY-12 (0.6 mL/L) and Kanamycin Sulfate respectively. Bacteria CE3 and CE4 have the highest sensitivity against Ampicillin Trihydrate and Tetracycline Hydrochloride respectively. The fresh skin of jackfruit treated with XY-12 (0.6 mL/L) and incubated at 4°C revealed high efficiency in microbial reduction at day 2 (100%) and 4 (99.72%)

Genomic analyses of five Roseivirga species: Insights into marine adaptation

To date, the genus Roseivirga consists of six species with one subspecies and is one of the least-studied genera among the family Flammeovirgaceae. In order to further explore this genus, the genome sequences of five Roseivirga spp. were compared and described in this study. The Roseivirga genomes have similar sizes in the range of 4.08–4.47 Mb with an average of 4.22 Mb. Several key proteins related to osmotic stress adaptation were identified in Roseivirga spp. including betaine transporter, choline dehydrogenase, and glutamate synthases. Significant amount of proteins associated with amino acid transport and metabolism were also present in Roseivirga genome. All five Roseivirga spp. were able to grow in medium contained casamino acids (mixture of amino acids) as sole carbon or nitrogen sources. Taken together, these findings suggested the potential role of Roseivirga in decomposing organic nitrogen matter in marine environment

Draft genome sequence of Vitellibacter vladivostokensis KMM 3516T: a protease-producing bacterium

Type strain Vitellibacter vladivostokensis KMM 3516T (= NBRC 16718T) belongs to the phylum Cytophaga–Flavobacterium–Bacteroides. To date, no genomes of the Vitellibacter spp. have been reported, and their metabolic pathways are unknown. This study reports the draft genome sequence of V. vladivostokensis. Moreover, mining of genes associated with proteolytic enzymes was performed to provide insights for further enzyme characterization