A novel pair of inducible expression vectors for use in Methylobacterium extorquens

Background: Due to the ever increasing use of diverse microbial taxa in basic research and industrial settings, there is a growing need for genetic tools to alter the physiology of these organisms. In particular, there is a dearth of inducible expression systems available for bacteria outside commonly used γ-proteobacteria, such as Escherichia coli or Pseudomonas species. To this end, we have sought to develop a pair of inducible expression vectors for use in the α-proteobacterium Methylobacterium extorquens, a model methylotroph. Findings: We found that the P R promoter from rhizobial phage 16-3 was active in M. extorquens and engineered the promoter to be inducible by either p-isopropyl benzoate (cumate) or anhydrotetracycline. These hybrid promoters, P R/cmtO and P R/tetO, were found to have high levels of expression in M. extorquens with a regulatory range of 10-fold and 30-fold, respectively. Compared to an existing cumate-inducible (10-fold range), high-level expression system for M. extorquens, P R/cmtO and P R/tetO have 33% of the maximal activity but were able to repress gene expression 3 and 8-fold greater, respectively. Both promoters were observed to exhibit homogeneous, titratable activation dynamics rather than on-off, switch-like behavior. The utility of these promoters was further demonstrated by complementing loss of function of ftfL - essential for growth on methanol - where we show P R/tetO is capable of not only fully complementing function but also producing a conditional null phenotype. These promoters have been incorporated into a broad-host-range backbone allowing for potential use in a variety of bacterial hosts. Conclusions: We have developed two novel expression systems for use in M. extorquens. The expression range of these vectors should allow for increased ability to explore cellular physiology in M. extorquens. Further, the P R/tetO promoter is capable of producing conditional null phenotypes, previously unattainable in M. extorquens. As both expression systems rely on the use of membrane permeable inducers, we suspect these expression vectors will be useful for ectopic gene expression in numerous proteobacteria

Can Community Policing Increase Residents\u27 Informal Social Control? Testing the Impact of the Chicago Alternative Policing Strategy

This study examines whether community policing can build informal social control. Specifically, this paper assesses the impact of the Chicago Alternative Policing Strategy (CAPS) in Chicago neighborhoods. The data for this research are drawn from both the Community Survey of the Project on Human Development in Chicago Neighborhoods (PHDCN) and the CAPS Prototype Panel Survey. Bivariate and multivariate methods are used to analyze data gathered from 8782 residents nested within 343 neighborhood clusters. Initially, community policing was found to increase informal social control, but this effect was rendered non-significant after controlling for theoretically and empirically relevant variables. Several social (dis)organization variables, as well as satisfaction with policing services, yielded significant effects in a multilevel regression model. Further analysis found that the community policing effect on informal social control was mediated through satisfaction with the police. These findings indicate indirect support for the ability of community policing to build informal social control and suggest that general satisfaction with the police is important to neighborhood crime control strategies

The role of religion in the longer-range future, April 6, 7, and 8, 2006

This repository item contains a single issue of the Pardee Conference Series, a publication series that began publishing in 2006 by the Boston University Frederick S. Pardee Center for the Study of the Longer-Range Future. This conference that took place during April 6, 7, and 8, 2006. Co-organized by David Fromkin, Director, Frederick S. Pardee Center for the Study of the Longer-Range Future, and Ray L. Hart, Dean ad interim Boston University School of TheologyThe conference brought together some 40 experts from various disciplines to ponder upon the “great dilemma” of how science, religion, and the human future interact. In particular, different panels looked at trends in what is happening to religion around the world, questions about how religion is impacting the current political and economic order, and how the social dynamics unleashed by science and by religion can be reconciled.Carnegie Council on Ethics and International Affair

A reappraisal of the Carboniferous macrofloras of the Zonguldak – Amasra Coal Basin, north-western Turkey

The Zonguldak – Amasra Coal Basin in north-western Turkey has Carboniferous terrestrial deposits ranging in age from Arnsbergian to late Asturian or possibly early Cantabrian. They yield macrofloras that allow detailed biostratigraphical correlations with sequences in Europe. These correlations suggest there are substantial gaps in the Zonguldak – Amasra succession, with middle to upper Namurian, upper Langsettian, Duckmantian and lower Asturian strata apparently being missing. This in turn suggests the area was subjected to significant tectonic instability during Pennsylvanian (late Carboniferous) times and that this might have been instrumental in initiating the progressive change in composition and eventual collapse of the coal swamp biome across Variscan Euramerica during Westphalian times.</p

Race/ethnicity, citizenship status, and crime examined through trauma experiences among young adults in the United States

Race/ethnicity, citizenship status, and trauma, have significant impact on delinquency and crime outcomes; though the rea- sons for some expected and unexpected crime pathways are still unanswered. Using data from the National Longitudinal Survey of Youth 1997 (n = 7,103), this study found the follow- ing results: no difference in the likelihood of engagement in delinquency and crime between blacks and whites; cumulative trauma increased delinquency and crime rates for all racial and ethnic groups; racial and ethnic minority groups compared to whites reported a significantly higher level of child- hood trauma experiences; and native-born female immigrant groups (but not male) were more likely to engage in delin- quency and crime than first-generation female immigrant groups. Implications and recommendations are set forth

First Estimates of the Radiative Forcing of Aerosols Generated from Biomass Burning using Satellite Data

Collocated measurements from the Advanced Very High Resolution Radiometer (AVHRR) and the Earth Radiation Budget Experiment (ERBE) scanner are used to examine the radiative forcing of atmospheric aerosols generated from biomass burning for 13 images in South America. Using the AVHRR, Local Area Coverage (LAC) data, a new technique based on a combination of spectral and textural measures is developed for detecting these aerosols. Then, the instantaneous shortwave, longwave, and net radiative forcing values are computed from the ERBE instantaneous scanner data. Results for the selected samples from 13 images show that the mean instantaneous net radiative forcing for areas with heavy aerosol loading is about -36 W/sq m and that for the optically thin aerosols are about -16 W/sq m. These results, although preliminary, provide the first estimates of radiative forcing of atmospheric aerosols from biomass burning using satellite data

A cross-cultural, participatory approach for measuring and cultivating resilience on small and medium farms

One of the greatest leverage points in fostering the transition to sustainability can be found in the realm of food systems. The COVID-19 pandemic has brought into sharp focus the critical importance of small farm resilience to the well-being of communities around the world. We explored the role of small and medium farms in promoting sustainable social-agricultural systems, and investigated how the resilience of these farms can be both measured and amplified. W e integrated concepts from the fields of food systems sustainability and resilience, agroecology, and positive deviance to identify indicators that can help measure and track farm resilience. Our aims were to 1) investigate the diversity of ecological and social factors that impact the vulnerability, resilience, and long-term health of small-medium farms and farm systems, exploring similarities and differences between the two cultures/geographies; 2) incorporate farmers’ personal experiences together with knowledge in the academic literature to enrich understanding about food systems sustainability; and 3) develop an indicator tool for evaluating farm resilience that empowers farmers to both assess their local farm system and to implement and document change over time. Our research process employed a cross-cultural, mixed-methods, Participatory Action Research (PAR) approach that engaged farmers from diverse geographic settings in Puerto Rico and Vermont. The project was organized into four phases implemented over a six month period from July through December 2020. These included identifying farmer participants, selecting key farm resilience frameworks, distilling a preliminary list of indicators, and validating those indicators with farmer feedback through surveys, interviews, and group meetings. The phases culminated in the creation of a Farm Resilience Tool for conducting rapid assessments using 20 indicators organized into four categories of Growth Mindset, Strong Relationships, Sustainable Farming Practices, and Sustainable Business Management. We further outlined a proposal for how to implement and refine the tool with farmer participants through subsequent PAR activities. Refinement is essential, given that our proposal is place-based and local, yet also modular and globally scalable to help advance planetary sustainability. More research is necessary to understand potential tradeoffs and synergies that can occur from trying to optimize multiple outcomes in tandem, and how to transition small farm resilience to broader-scale landscape planning and management strategies. Among the important lessons learned from this project are A) the importance of farmer-to-farmer knowledge sharing, B) the value of an authentic and reciprocal PAR process in expanding the range and depth of understanding beyond the academic literature, C) the utility of integrating positive deviants for helping identify indicators and examples of resilience outside the box of traditional thinking, and D) the rich interaction across cultures and geographies that enlivens the research and enlightens the outcomes. All these lessons converge on the critical insight that sustainability is ultimately about the quality of relationships within food systems. Thus, food systems research must integrate objective and subjective methods in order to cultivate the relational synergy needed to address and transcend the complex problems we face in the 21st Century

A Randomized Controlled Trial to Compare Computer-assisted Motivational Intervention with Didactic Educational Counseling to Reduce Unprotected Sex in Female Adolescents

Study Objective: To examine a computer-assisted, counselor-guided motivational intervention (CAMI) aimed at reducing the risk of unprotected sexual intercourse. Design, Setting, Participants, Interventions, and Main Outcome Measures: We conducted a 9-month, longitudinal randomized controlled trial with a multisite recruitment strategy including clinic, university, and social referrals, and compared the CAMI with didactic educational counseling in 572 female adolescents with a mean age of 17 years (SD = 2.2 years; range = 13-21 years; 59% African American) who were at risk for pregnancy and sexually transmitted diseases. The primary outcome was the acceptability of the CAMI according to self-reported rating scales. The secondary outcome was the reduction of pregnancy and sexually transmitted disease risk using a 9-month, self-report timeline follow-back calendar of unprotected sex. Results: We conducted a 9-month, longitudinal randomized controlled trial with a multisite recruitment strategy including clinic, university, and social referrals, and compared the CAMI with didactic educational counseling in 572 female adolescents with a mean age of 17 years (SD = 2.2 years; range = 13-21 years; 59% African American) who were at risk for pregnancy and sexually transmitted diseases. The primary outcome was the acceptability of the CAMI according to self-reported rating scales. The secondary outcome was the reduction of pregnancy and sexually transmitted disease risk using a 9-month, self-report timeline follow-back calendar of unprotected sex. Conclusion: Among those who completed the intervention, the CAMI reduced unprotected sex among an at-risk, predominantly minority sample of female adolescents. Modification of the CAMI to address methodological issues that contributed to a high drop-out rate are needed to make the intervention more acceptable and feasible for use among sexually active predominantly minority, at-risk, female adolescents