LOS PROCESOS DE EVALUACIÓN DE LA CALIDAD EN LOS ESTUDIOS DE ENFERMERÍA EN EL CONTEXTO DEL ESPACIO EUROPEO DE EDUCACIÓN SUPERIOR.

The objective of this article is to make an approach between the institutional evaluation development in our country, the new programmes and the challenges that are being proposed in the qualifications. The European convergence has one of the most important bases in the quality process of the formation programmes and centres. During the last few years, all around Europe, Spain included, it has been developed a severe quality process which facilitate the introduction of improvements and the corresponding explanations to society.El objetivo de este artículo es realizar una aproximación sobre el desarrollo de la evaluación institucional en nuestro país, nuevos programas y retos que se están planteando en las titulaciones. La convergencia europea tiene en los procesos de calidad de los programas de formación y de los centros uno de los pilares más importantes. En Europa, incluida España, se han desarrollado en los últimos años intensos procesos de evaluación de la calidad propiciando la introducción de mejoras y la rendición de cuentas a la sociedad

Hereditary Leiomyomatosis and Renal Cell Cancer Syndrome in Spain: Clinical and Genetic Characterization

Simple Summary Hereditary leiomyomatosis and renal cell cancer (HLRCC) syndrome is a very rare hereditary disorder characterized by cutaneous leiomyomas (CLMs), uterine leiomyomas (ULMs), renal cysts (RCys) and renal cell cancer (RCC), with no data on its prevalence worldwide. No genotype-phenotype associations have been described. The aim of our study was to describe the genotypic and phenotypic features of the largest series of patients with HLRCC from Spain reported to date. Of 27 FH germline pathogenic variants, 12 were not previously reported in databases. Patients with missense pathogenic variants showed higher frequencies of CLMs, ULMs and RCys, than those with loss-of-function variants. The frequency of RCCs (10.9%) was lower than those reported in the previously published series. Hereditary leiomyomatosis and renal cell cancer syndrome (HLRCC) is a very rare hereditary disorder characterized by cutaneous leiomyomas (CLMs), uterine leiomyomas (ULMs), renal cysts (RCys) and renal cell cancers (RCCs). We aimed to describe the genetics, clinical features and potential genotype-phenotype associations in the largest cohort of fumarate hydratase enzyme mutation carriers known from Spain using a multicentre, retrospective study of individuals with a genetic or clinical diagnosis of HLRCC. We collected clinical information from medical records, analysed genetic variants and looked for genotype-phenotype associations. Analyses were performed using R 3.6.0. software. We included 197 individuals: 74 index cases and 123 relatives. CLMs were diagnosed in 65% of patients, ULMs in 90% of women, RCys in 37% and RCC in 10.9%. Twenty-seven different pathogenic variants were detected, 12 (44%) of them not reported previously. Patients with missense pathogenic variants showed higher frequencies of CLMs, ULMs and RCys, than those with loss-of-function variants (p = 0.0380, p = 0.0015 and p = 0.024, respectively). This is the first report of patients with HLRCC from Spain. The frequency of RCCs was lower than those reported in the previously published series. Individuals with missense pathogenic variants had higher frequencies of CLMs, ULMs and RCys

The quality evaluation processes at the nursing studies in the frame of the European Space of Higher Education.

El objetivo de este artículo es realizar una aproximación sobre el desarrollo de la evaluación institucional en nuestro país, nuevos programas y retos que se están planteando en las titulaciones. La convergencia europea tiene en los procesos de calidad de los programas de formación y de los centros uno de los pilares más importantes. En Europa, incluida España, se han desarrollado en los últimos años intensos procesos de evaluación de la calidad propiciando la introducción de mejoras y la rendición de cuentas a la sociedad.ABSTRACT The objective of this article is to make an approach between the institutional evaluation development in our country, the new programmes and the challenges that are being proposed in the qualifications. The European convergence has one of the most important bases in the quality process of the formation programmes and centres. During the last few years, all around Europe, Spain included, it has beendeveloped a severe quality process which facilitate the introduction of improvements and the corresponding explanations to society

Peritoneal fluid reduces angiogenesis-related microRNA expression in cell cultures of endometrial and endometriotic tissues from women with endometriosis.

UNLABELLED: Endometriosis, defined as the presence of endometrium outside the uterus, is one of the most frequent gynecological diseases. It has been suggested that modifications of both endometrial and peritoneal factors could be implicated in this disease. Endometriosis is a multifactorial disease in which angiogenesis and proteolysis are dysregulated. MicroRNAs (miRNAs) are small non-coding RNAs that regulate the protein expression and may be the main regulators of angiogenesis. Our hypothesis is that peritoneal fluid from women with endometriosis could modify the expression of several miRNAs that regulate angiogenesis and proteolysis in the endometriosis development. The objective of this study has been to evaluate the influence of endometriotic peritoneal fluid on the expression of six miRNAs related to angiogenesis, as well as several angiogenic and proteolytic factors in endometrial and endometriotic cell cultures from women with endometriosis compared with women without endometriosis. METHODS: Endometrial and endometriotic cells were cultured and treated with endometriotic and control peritoneal fluid pools. We have studied the expression of six miRNAs (miR-16, -17-5p, -20a, -125a, -221, and -222) by RT-PCR and protein and mRNA levels of vascular endothelial growth factor-A, thrombospondin-1, urokinase plasminogen activator and plasminogen activator inhibitor-1 by ELISA and qRT-PCR respectively. RESULTS: Control and endometriotic peritoneal fluid pools induced a significant reduction of all miRNAs levels in endometrial and endometriotic cell cultures. Moreover, both peritoneal fluids induced a significant increase in VEGF-A, uPA and PAI-1 protein levels in all cell cultures without significant increase in mRNA levels. Endometrial cell cultures from patients treated with endometriotic peritoneal fluid showed lower expression of miRNAs and higher expression of VEGF-A protein levels than cultures from controls. In conclusion , this "in vitro" study indicates that peritoneal fluid from women with endometriosis modulates the expression of miRNAs that could contribute to the angiogenic and proteolytic disequilibrium observed in this disease

Peritoneal fluid (PF) effects on VEGF-A and TSP-1 expression in stromal cell cultures from control endometrial tissue, and patient endometrial and endometriotic tissues from women with endometriosis.

<p>ØPF, without PF; CPF, control PF; EPF, endometriotic PF. Data are expressed as Mean ± SEM. * <i>p</i><0.05; ** <i>p</i><0.01; *** <i>p</i><0.001; vs ØPF from same tissue culture, ψ <i>p</i><0.05 vs ØPF from control endometrium culture. A: VEGF-A protein; B: VEGF-A mRNA; C: TSP-1 protein; D: TSP-1 mRNA.</p

Peritoneal fluid (PF) effects on miRNA expression in stromal cell cultures from control endometrial tissue, and patient endometrial and endometriotic tissues from women with endometriosis.

<p>ØPF, without PF; CPF, control PF; EPF, endometriotic PF. *<i>p</i><0.05; **<i>p</i><0.01; <i>p</i><0.001 vs ØPF same tissue culture. miRNA expression is presented as fold change relative to control stromal cell culture without peritoneal fluid (control endometrium ØPF = 1). A: miR-16; B: miR-17-5p; C: miR-20a; D: miR-125a; E: miR-221; F: miR-222.</p

Correlation between changes in miR-16 vs. VEGF-A protein levels in eutopic endometrial (A) and endometriotic cell cultures (B) from women with endometriosis after treatment with both peritoneal fluid pools compared to cell cultures without peritoneal fluid treatment.

<p>Change: differences between parameter levels with and without peritoneal fluid treatment.</p

Peritoneal fluid (PF) effects on uPA and PAI-1 expression in stromal cell cultures from control endometrial tissue, and patient endometrial and endometriotic tissues from women with endometriosis.

<p>ØPF, without PF; CPF, control PF; EPF, endometriotic PF. Data are expressed as Mean ± SEM. * <i>p</i><0.05; ** <i>p</i><0.01; *** <i>p</i><0.001; vs ØPF from same tissue culture, ψ <i>p</i><0.05 vs ØPF from control endometrium culture. A: uPA protein; B: uPA mRNA; C: PAI-1 protein; D: PAI-1 mRNA.</p