Synthesis, spectral studies, in vitro and molecular docking studies of novel hydrazinyl carbothioamide derivatives

Five novel compounds possessing hydrazinyl carbothioamide moiety were designed and synthesized. All the compounds were tested for in vitro biological activities. Most of the tested compounds displayed strong antibacterial and antifungal activities. Molecular docking studies suggested that the hydrazinyl carbothioamide moiety of compounds (6–10) can in general be accommodated the binding pocket of the breast cancer protein (1JNX) and are responsible for the activity of the whole of the molecule. The docking results provide a new insight into the design of hydrazinyl carbothioamide derivatives as breast cancer drug

A facile, solvent and catalyst free, microwave assisted one pot synthesis of hydrazinyl thiazole derivatives

A rapid synthesis of hydrazinyl thiazoles under solvent and catalyst free condition is reported within 30 s. A series of aryl ketones/4-benzoyl pyridine thiosemicarbazone, thiosemicarbazide and α-haloketones were used. This is an environmentally benign microwave assisted and efficient method for rapid synthesis of hydrazinyl thiazoles

Synthesis, spectral characterization and biological evaluation of 1-thiocarbamoyl-3-phenyl-5-hydroxy-5-(-2-pyridyl)-4-pyrazolines via Michael addition

A series of 1-thiocarbamoyl-3-phenyl-5-hydroxy-5-(2-pyridyl)-4-pyrazolines derivatives have been synthesized using sodium acetate as a catalyst. The spectral characterization and structure of 1-thiocarbamoyl-3-phenyl-5-hydroxy-5-(2-pyridyl)-4-pyrazolines are reported. Spectral techniques employed include 1H NMR, 13C NMR, 1H–1H COSY, HSQC, HMBC, D2O exchange, Mass and IR. Compounds 12–22 exhibited potent antibacterial activity against Salmonella typhi and Pseudomonas aeruginosa whereas the same set of compounds exerted potent antifungal activity against Aspergillus niger and Aspergillus fumigatus

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Not AvailableYellow leaf (YL) caused by Sugarcane yellow leaf virus (SCYLV) is a serious viral disease affecting production and productivity in many ruling sugarcane varieties in India. Usually the characteristic disease symptoms appear during maturity phases of the crop; also many of the infected varieties don’t exhibit disease symptoms and disease expression is influenced by virus titre and other factors including prevailing climate. The present study was taken up to quantify and compare SCYLV in meristem derived tissue culture raised in vitro plantlets and asymptomatic sugarcane plants in RT-qPCR by relative standard curve method. The standard curve was prepared with serial dilutions of plasmid standards from 3x107 to 3x103 copy numbers of virus target gene. In this assay, copy number of virus population in in vitro plantlets and asymptomatic plants was estimated from 20,314.58 to 4,330.87 and from 8.96 to 0.27 million copy of viruses, respectively. Relative expression level of the virus between in vitro plantlets and asymptomatic plants was in the ratio of 73.7:243393.1 based on 2^ (-(ΔΔCt)). The results clearly established that meristem derived tissue culture significantly reduced SCYLV population and it is concluded that the relative standard curve method efficiently detect the copy numbers of target virus in different sugarcane samples.Not Availabl

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Not AvailableYellow leaf (YL) of sugarcane caused by Sugarcane yellow leaf virus (ScYLV, a Polerovirus of the Luteoviridae family) is a serious disease affecting the crop production and productivity in India. Although impact of the disease on cane growth is observed, no systematic study has been done so far for the tropical Asian region to establish its impact on various physiological parameters, cane yield and juice quality. We have assessed physiological parameters in symptomatic and asymptomatic plants of 10 different cultivars and a genotype. In addition, similar comparisons were made between virus infected and virus free plants derived through meristem culture. Our studies established that among several physiological parameters, photosynthetic rate (A), stomatal conductance (gs) and SPAD meter values were significantly reduced in cultivars severely infected with ScYLV. Virus-infected cultivars exhibited significant reduction in growth/yield parameters, viz. stalk height, stalk thickness and number of internodes. Plant growth reductions were found to be 42.9, 42.3 and 38.9% in susceptible cultivars CoPant 84211, Co 86032 and CoC 671, respectively. In addition to reduction in stalk weight, height and girth, YL disease also reduced juice yield in the affected canes up to 34.15%. Similarly, comparison of diseased (virus-infected) and virus-free plants derived through meristem culture also revealed a drastic reduction in cane growth/physiological parameters and juice yield due to virus infection. The present study is the first comprehensive report demonstrating that YL disease caused by ScYLV seriously affects cane and juice yield in major sugarcane varieties under tropical climatic conditions (India). Consequently, this situation warrants a massive programme to provide healthy seed material and initiate breeding for YL resistance in sugarcane.Not Availabl