Life Tables of Bactrocera cucurbitae (Coquillett) (Diptera: Tephritidae): with a Mathematical Invalidation for Applying the Jackknife Technique to the Net Reproductive Rate

Life table data for the melon fly, Bactrocera cucurbitae (Coquillett), reared on cucumber (Cucumis sativus L.) were collected under laboratory and simulated field conditions. Means and standard errors of life table parameters were estimated for two replicates using the jackknife technique. At 25ºC, the intrinsic rates of increase (_r_) found for the two replicates were 0.1354 and 0.1002 day-1, and the net reproductive rates (_R_~0~) were 206.3 and 66.0 offspring, respectively. When the cucumbers kept under simulated field conditions were covered with leaves, the _r_ and _R_~0~ for the two replicates were 0.0935 and 0.0909 day-1, 17.5 and 11.4 offspring, respectively. However, when similar cucumbers were left uncovered, the _r_ and _R_~0~ for the two replicates were 0.1043 and 0.0904 day-1, and 27.7 and 10.1 offspring, respectively. Our results revealed that considerable variability between replicates in both laboratory and field conditions is possible; this variability should be taken into consideration in data collection and application of life tables. Mathematical analysis has demonstrated that applying the jackknife technique results in unrealistic pseudo-_R_~0~ and overestimation of its variance. We suggest that the jackknife technique should not be used for the estimation of variability of _R_~0~

"An Econometric Analysis of SARS and Avian Flu on International Tourist Arrivals to Asia"

This paper compares the impacts of SARS and human deaths arising from Avian Flu on international tourist arrivals to Asia. The effects of SARS and human deaths from Avian Flu will be compared directly according to human deaths. The nature of the short run and long run relationship is examined empirically by estimating a static line fixed effect model and a difference transformation dynamic model, respectively. Empirical results from the static fixed effect and difference transformation dynamic models are consistent, and indicate that both the short run and long run SARS effect have a more significant impact on international tourist arrivals than does Avian Flu. In addition, the effects of deaths arising from both SARS and Avian Flu suggest that SARS is more important to international tourist arrivals than is Avian Flu. Thus, while Avian Flu is here to stay, its effect is currently not as significant as that of SARS.

A balanced homodyne detector for high-rate Gaussian-modulated coherent-state quantum key distribution

We discuss excess noise contributions of a practical balanced homodyne detector in Gaussian-modulated coherent-state (GMCS) quantum key distribution (QKD). We point out the key generated from the original realistic model of GMCS QKD may not be secure. In our refined realistic model, we take into account excess noise due to the finite bandwidth of the homodyne detector and the fluctuation of the local oscillator. A high speed balanced homodyne detector suitable for GMCS QKD in the telecommunication wavelength region is built and experimentally tested. The 3dB bandwidth of the balanced homodyne detector is found to be 104MHz and its electronic noise level is 13dB below the shot noise at a local oscillator level of 8.5*10^8 photon per pulse. The secure key rate of a GMCS QKD experiment with this homodyne detector is expected to reach Mbits/s over a few kilometers.Comment: 22 pages, 11 figure

An econometric analysis of SARS and Avian flu on international tourist arrivals to Asia

This paper compares the impacts of SARS and human deaths arising from Avian Flu on international tourist arrivals to Asia. The effects of SARS and human deaths from Avian Flu will be compared directly according to human deaths. The nature of the short run and long run relationship is examined empirically by estimating a static line fixed effect model and a difference transformation dynamic model, respectively. Empirical results from the static fixed effect and difference transformation dynamic models are consistent, and indicate that both the short run and long run SARS effect have a more significant impact on international tourist arrivals than does Avian Flu. In addition, the effects of deaths arising from both SARS and Avian Flu suggest that SARS is more important to international tourist arrivals than is Avian Flu. Thus, while Avian Flu is here to stay, its effect is currently not as significant as that of SARS

Rechargeable Li/Cl2_2 battery down to -80 {\deg}C

Low temperature rechargeable batteries are important to life in cold climates, polar/deep-sea expeditions and space explorations. Here, we report ~ 3.5 - 4 V rechargeable lithium/chlorine (Li/Cl2) batteries operating down to -80 {\deg}C, employing Li metal negative electrode, a novel CO2 activated porous carbon (KJCO2) as the positive electrode, and a high ionic conductivity (~ 5 to 20 mS cm-1 from -80 {\deg}C to 25 {\deg}C) electrolyte comprised of 1 M aluminum chloride (AlCl3), 0.95 M lithium chloride (LiCl), and 0.05 M lithium bis(fluorosulfonyl)imide (LiFSI) in low melting point (-104.5 {\deg}C) thionyl chloride (SOCl2). Between room-temperature and -80 {\deg}C, the Li/Cl2 battery delivered up to ~ 30,000 - 4,500 mAh g-1 first discharge capacity and a 1,200 - 5,000 mAh g-1 reversible capacity (discharge voltages in ~ 3.5 to 3.1 V) over up to 130 charge-discharge cycles. Mass spectrometry and X-ray photoelectron spectroscopy (XPS) probed Cl2 trapped in the porous carbon upon LiCl electro-oxidation during charging. At lower temperature down to -80 {\deg}C, SCl2/S2Cl2 and Cl2 generated by electro-oxidation in the charging step were trapped in porous KJCO2 carbon, allowing for reversible reduction to afford a high discharge voltage plateau near ~ 4 V with up to ~ 1000 mAh g-1 capacity for SCl2/S2Cl2 reduction and up to ~ 4000 mAh g-1 capacity at ~ 3.1 V plateau for Cl2 reduction. Towards practical use, we made CR2032 Li/Cl2 battery cells to drive digital watches at -40 {\deg}C and light emitting diode at -80 {\deg}C, opening Li/Cl2 secondary batteries for ultra-cold conditions

Transcriptionally active HERV-H retrotransposons demarcate topologically associating domains in human pluripotent stem cells.

Chromatin architecture has been implicated in cell type-specific gene regulatory programs, yet how chromatin remodels during development remains to be fully elucidated. Here, by interrogating chromatin reorganization during human pluripotent stem cell (hPSC) differentiation, we discover a role for the primate-specific endogenous retrotransposon human endogenous retrovirus subfamily H (HERV-H) in creating topologically associating domains (TADs) in hPSCs. Deleting these HERV-H elements eliminates their corresponding TAD boundaries and reduces the transcription of upstream genes, while de novo insertion of HERV-H elements can introduce new TAD boundaries. The ability of HERV-H to create TAD boundaries depends on high transcription, as transcriptional repression of HERV-H elements prevents the formation of boundaries. This ability is not limited to hPSCs, as these actively transcribed HERV-H elements and their corresponding TAD boundaries also appear in pluripotent stem cells from other hominids but not in more distantly related species lacking HERV-H elements. Overall, our results provide direct evidence for retrotransposons in actively shaping cell type- and species-specific chromatin architecture

Concurrent image-guided intensity modulated radiotherapy and chemotherapy following neoadjuvant chemotherapy for locally advanced nasopharyngeal carcinoma

<p>Abstract</p> <p>Background</p> <p>To evaluate the experience of induction chemotherapy followed by concurrent chemoradiationwith helical tomotherapy (HT) for nasopharyngeal carcinoma (NPC).</p> <p>Methods</p> <p>Between August 2006 and December 2009, 28 patients with pathological proven nonmetastatic NPC were enrolled. All patients were staged as IIB-IVB. Patients were first treated with 2 to 3 cycles of induction chemotherapy with EP-HDFL (Epirubicin, Cisplatin, 5-FU, and Leucovorin). After induction chemotherapy, weekly based PFL was administered concurrent with HT. Radiation consisted of 70 Gy to the planning target volumes of the primary tumor plus any positive nodal disease using 2 Gy per fraction.</p> <p>Results</p> <p>After completion of induction chemotherapy, the response rates for primary and nodal disease were 96.4% and 80.8%, respectively. With a median follow-up after 33 months (Range, 13-53 months), there have been 2 primary and 1 nodal relapse after completion of radiotherapy. The estimated 3-year progression-free rates for local, regional, locoregional and distant metastasis survival rate were 92.4%, 95.7%, 88.4%, and 78.0%, respectively. The estimated 3-year overall survival was 83.5%. Acute grade 3, 4 toxicities for xerostomia and dermatitis were only 3.6% and 10.7%, respectively.</p> <p>Conclusion</p> <p>HT for locoregionally advanced NPC is feasible and effective in regard to locoregional control with high compliance, even after neoadjuvant chemotherapy. None of out-field or marginal failure noted in the current study confirms the potential benefits of treating NPC patients by image-guided radiation modality. A long-term follow-up study is needed to confirm these preliminary findings.</p

Peptide modified mesenchymal stem cells as targeting delivery system transfected with miR-133b for the treatment of cerebral ischemia.

Mesenchymal stem cells (MSCs) have been regarded as potential targeting vehicles and demonstrated to exert therapeutic benefits for brain diseases. Direct homing to diseased tissue is crucial for stem cell-based therapy. In this study, a peptide-based targeting approach was established to enhance cell homing to cerebral ischemic lesion. Palmitic acid-peptide painted onto the cell membrane was able to direct MSCs to ischemic tissues without any observed cell cytotoxicity and influence on differentiation, thus reducing accumulation of cells in peripheral organs and increasing engraftment of cells in the targeted tissues. With enhanced cell homing, MSCs were used to deliver miR-133b to increase the expression level of miR-133b in an ischemic lesion and further improve therapeutic effects. This study is the first to develop MSCs co-modified with targeting peptide and microRNAs as potential targeting therapeutic agents. This targeting delivery system is expected to be applicable to other cell types and other diseases aside from stroke

Apoptosis signal-regulating kinase 1 mediates denbinobin-induced apoptosis in human lung adenocarcinoma cells

In the present study, we explore the role of apoptosis signal-regulating kinase 1 (ASK1) in denbinobin-induced apoptosis in human lung adenocarcinoma (A549) cells. Denbinobin-induced cell apoptosis was attenuated by an ASK1 dominant-negative mutant (ASK1DN), two antioxidants (N-acetyl-L-cysteine (NAC) and glutathione (GSH)), a c-Jun N-terminal kinase (JNK) inhibitor (SP600125), and an activator protein-1 (AP-1) inhibitor (curcumin). Treatment of A549 cells with denbinobin caused increases in ASK1 activity and reactive oxygen species (ROS) production, and these effects were inhibited by NAC and GSH. Stimulation of A549 cells with denbinobin caused JNK activation; this effect was markedly inhibited by NAC, GSH, and ASK1DN. Denbinobin induced c-Jun phosphorylation, the formation of an AP-1-specific DNA-protein complex, and Bim expression. Bim knockdown using a bim short interfering RNA strategy also reduced denbinobin-induced A549 cell apoptosis. The denbinobin-mediated increases in c-Jun phosphorylation and Bim expression were inhibited by NAC, GSH, SP600125, ASK1DN, JNK1DN, and JNK2DN. These results suggest that denbinobin might activate ASK1 through ROS production to cause JNK/AP-1 activation, which in turn induces Bim expression, and ultimately results in A549 cell apoptosis