5,422 research outputs found
Bioproduction of L-piperazic acid in gram scale using Aureobasidium melanogenum
Currently, piperazic acid is chemically synthesized using ecologically unfriendly processes. Microbial synthesis from glucose is an attractive alternative to chemical synthesis. In this study, we report the production of L-piperazic acid via microbial fermentation with the first engineered fungal strain of Aureobasidium melanogenum; this strain was constructed by chassis development, genetic element reconstitution and optimization, synthetic rewiring and constitutive genetic circuit reconstitution, to build a robust L-piperazic acid synthetic cascade. These genetic modifications enable A. melanogenum to directly convert glucose to L-piperazic acid without relying on the use of either chemically synthesized precursors or harsh conditions. This bio-based process overcomes the shortcomings of the conventional synthesis routes. The ultimately engineered strain is a very high-efficient cell factory that can excrete 1.12 ± 0.05 g l-1 of L-piperazic acid after a 120-h 10.0-l fed-batch fermentation; this is the highest titre of L-piperazic acid reported using a microbial cell factory
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Malaria parasite translocon structure and mechanism of effector export.
The putative Plasmodium translocon of exported proteins (PTEX) is essential for transport of malarial effector proteins across a parasite-encasing vacuolar membrane into host erythrocytes, but the mechanism of this process remains unknown. Here we show that PTEX is a bona fide translocon by determining structures of the PTEX core complex at near-atomic resolution using cryo-electron microscopy. We isolated the endogenous PTEX core complex containing EXP2, PTEX150 and HSP101 from Plasmodium falciparum in the 'engaged' and 'resetting' states of endogenous cargo translocation using epitope tags inserted using the CRISPR-Cas9 system. In the structures, EXP2 and PTEX150 interdigitate to form a static, funnel-shaped pseudo-seven-fold-symmetric protein-conducting channel spanning the vacuolar membrane. The spiral-shaped AAA+ HSP101 hexamer is tethered above this funnel, and undergoes pronounced compaction that allows three of six tyrosine-bearing pore loops lining the HSP101 channel to dissociate from the cargo, resetting the translocon for the next threading cycle. Our work reveals the mechanism of P. falciparum effector export, and will inform structure-based design of drugs targeting this unique translocon
A similarity measure method of Chinese sentence structures
2005-2006 > Academic research: refereed > Publication in refereed journalVersion of RecordPublishe
Antiferromagnetic Critical Fluctuations in BaFeAs
Magnetic correlations near the magneto-structural phase transition in the
bilayer iron pnictide parent compound, BaFeAs, are measured. In close
proximity to the antiferromagnetic phase transition in BaFeAs, a
crossover to three dimensional critical behavior is anticipated and has been
preliminarily observed. Here we report complementary measurements of
two-dimensional magnetic fluctuations over a broad temperature range about
T. The potential role of two-dimensional critical fluctuations in the
magnetic phase behavior of BaFeAs and their evolution near the
anticipated crossover to three dimensional critical behavior and long-range
order are discussed.Comment: 6 pages, 4 figures; Accepted for publication in Physical Review
Thorium-doping induced superconductivity up to 56 K in Gd1-xThxFeAsO
Following the discovery of superconductivity in an iron-based arsenide
LaO1-xFxFeAs with a superconducting transition temperature (Tc) of 26 K[1], Tc
was pushed up surprisingly to above 40 K by either applying pressure[2] or
replacing La with Sm[3], Ce[4], Nd[5] and Pr[6]. The maximum Tc has climbed to
55 K, observed in SmO1-xFxFeAs[7, 8] and SmFeAsO1-x[9]. The value of Tc was
found to increase with decreasing lattice parameters in LnFeAsO1-xFx (Ln stands
for the lanthanide elements) at an apparently optimal doping level. However,
the F- doping in GdFeAsO is particularly difficult[10,11] due to the lattice
mismatch between the Gd2O2 layers and Fe2As2 layers. Here we report observation
of superconductivity with Tc as high as 56 K by the Th4+ substitution for Gd3+
in GdFeAsO. The incorporation of relatively large Th4+ ions relaxes the lattice
mismatch, hence induces the high temperature superconductivity.Comment: 4 pages, 3 figure
Formation and evolution of epitaxial Co5Ge7Co5Ge7 film on Ge (001) surface by solid-state reaction in an in situ ultrahigh-vacuum transmission electron microscope
A thin metallic cobalt (Co) layer was deposited on a single-crystal Ge (001) surface at room temperature by the electron-beam evaporation of a pure Co metal source in an ultrahigh-vacuum transmission electron microscope. The formation and epitaxial growth of a cobalt germanide Co5Ge7Co5Ge7 phase on the Ge (001) surface was studied in situ by gradually heating the sample from room temperature to ∼ 350 °C∼350°C. The occurrence of an epitaxial hexagonal-close-packed Co and the reaction between Co and Ge were observed at ∼ 225 °C∼225°C. After annealing at ∼ 300 °C∼300°C for 26.5 h, a continuous epitaxial Co5Ge7Co5Ge7 film formed on the Ge (001) substrate. With further annealing at a higher temperature, the continuous Co5Ge7Co5Ge7 layer broke up and formed three-dimensional islands in order to relieve the strain energy in the epitaxial Co5Ge7Co5Ge7 layer. Two epitaxial relationships between Co5Ge7Co5Ge7 and Ge, i.e., Co5Ge7〈110〉(001)//Ge〈100〉(001)Co5Ge7〈110〉(001)∕∕Ge〈100〉(001) and Co5Ge7〈001〉(110)//Ge〈100〉(001)Co5Ge7〈001〉(110)∕∕Ge〈100〉(001) were found by electron diffraction.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/87837/2/211909_1.pd
57Fe Mossbauer spectroscopy and magnetic measurements of oxygen deficient LaFeAsO
We report on the magnetic behavior of oxygen deficient LaFeAsO1-x (x-0.10)
compound, prepared by one-step synthesis, which crystallizes in the tetragonal
(S.G. P4/nmm) structure at room temperature. Resistivity measurements show a
strong anomaly near 150 K, which is ascribed to the spin density wave (SDW)
instability. On the other hand, dc magnetization data shows paramagnetic-like
features down to 5 K, with an effective moment of 0.83 mB/Fe. 57Fe Mossbauer
studies (MS) have been performed at 95 and 200 K. The spectra at both
temperatures are composed of two sub-spectra. At 200 K the major one (88%), is
almost a singlet, and corresponds to those Fe nuclei, which have two oxygen
ions in their close vicinity. The minor one, with a large quadrupole splitting,
corresponds to Fe nuclei, which have vacancies in their immediate neighborhood.
The spectrum at 95 K, exhibits a broadened magnetic split major (84%)
sub-spectrum and a very small magnetic splitting in the minor subspectrum. The
relative intensities of the subspectra facilitate in estimating the actual
amount of oxygen vacancies in the compound to be 7.0(5)%, instead of the
nominal LaFeAsO0.90. These results, when compared with reported 57Fe MS of
non-superconducting LaFeAsO and superconducting LaFeAsO0.9F0.1, confirm that
the studied LaFeAsO0.93 is a superconductivity-magnetism crossover compound of
the newly discovered Fe based superconducting family.Comment: 7 pages text + Figs : Comments/suggestions welcome
([email protected]
Inelastic neutron scattering studies of Crystal Field Levels in PrOsAs
We use neutron scattering to study the Pr crystalline electric field
(CEF) excitations in the filled skutterudite PrOsAs. By comparing
the observed levels and their strengths under neutron excitation with the
theoretical spectrum and neutron excitation intensities, we identify the
Pr CEF levels, and show that the ground state is a magnetic
triplet, and the excited states ,
and are at 0.4, 13 and 23 meV, respectively. A comparison of the
observed CEF levels in PrOsAs with the heavy fermion superconductor
PrOsSb reveals the microscopic origin of the differences in the
ground states of these two filled skutterudites.Comment: 7 pages, 7 figure
Transient heat generation in a quantum dot under a step-like pulse bias
We study the transient heat generation in a quantum dot system driven by a
step-like or a square-shaped pulse bias. We find that a periodically
oscillating heat generation arises after adding the sudden bias. One
particularly surprising result is that there exists a heat absorption from the
zero-temperature phonon subsystem. Thus the phonon population in
non-equilibrium can be less than that of the equilibrium electron-phonon
system. In addition, we also ascertain the optimal conditions for the operation
of a quantum dot with the minimum heat generation.Comment: 6 pages, 4 figure
Boundary energy of the general open XXZ chain at roots of unity
We have recently proposed a Bethe Ansatz solution of the open spin-1/2 XXZ
quantum spin chain with general integrable boundary terms (containing six free
boundary parameters) at roots of unity. We use this solution, together with an
appropriate string hypothesis, to compute the boundary energy of the chain in
the thermodynamic limit.Comment: 22 pages, 6 figures; v2: some comments, a reference and a footnote
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