The complex of miRNA2861 and cell-penetrating, dimeric α-helical peptide accelerates the osteogenesis of mesenchymal stem cells

Abstract Background The restoration of the functional ability of mesenchymal stem cells (MSCs) using epigenetic modification is very promising for patients with weak osteogenesis ability. This study focused on the acceleration of osteogenesis from MSCs using microRNA (miRNA)2861 and a cell-penetrating peptide (CPP), LK. Methods We performed MSCs penetration test of complex between the LK peptides and miRNA 2861. Three different experiments were performed to investigate the effects of miRNA 2861 on osteogenic differentiation in MSCs: 1) intensity of alizarin red staining, which reflects the status of mineralization by osteoblasts; 2) gene expression related to osteoblast differentiation; and 3) confirmation of corresponding protein translation for comparison with RNA expression levels. Results We found that cLK effectively delivered miRNA 2861 into the cytoplasm of human MSCs and accelerated osteogenic differentiation from MSCs, as well as mineralization. Conclusion The complex of miRNA 2861 with LK may have a positive effect on the osteogenic differentiation from MSCs and mineralization. Therapies using miRNAs combined with LK may be good candidates for the augmentation of osteogenesis in patients

Early Outcome of Posterior Cervical Endoscopic Discectomy: An Alternative Treatment Choice for Physically/Socially Active Patients

Anterior cervical discectomy and fusion (ACDF) is currently the standard treatment for cervical disc disease. Some patients wish to be treated with a less invasive method, because of their social/physical situations. Here we present one method of treatments for socially/physically active patients. Three patients had triceps weakness and mild posterior neck pain. The offending lesions were at the C6-7 level. All were middle-aged soldiers with families. If conventional ACDF were performed, they would have to retire from the military according to the regulation. They had to be able to perform military drills after the treatment if they were going to be able to keep their jobs. Because of their social/physical situations, all wanted to choose method with that they could treat the disease and keep their jobs. For these reasons, the posterior cervical endoscopic discectomies were performed. Ruptured fragments were successfully removed in all. The arm pain improved by more than 90% in two patients by 7 days and in the other patient by 2 months, respectively (excellent outcome by Macnab's criteria). None of the operations caused instability. All of the patients are currently able to successfully perform their military drills without difficulty. The posterior cervical endoscopic discectomy may be a promising alternative for the physically/socially active patients

Pathological Classification of the Intramedullary Spinal Cord Tumors According to 2021 World Health Organization Classification of Central Nervous System Tumors, a Single-Institute Experience

According to the new 2021 World Health Organization (WHO) classification of tumors of the central nervous system (CNS) the classification of the primary intramedullary spinal cord tumors (IM-SCT) follows that of CNS tumors. However, since the genetics and methylation profile of ependymal tumors depend on the location of the tumor, the ‘spinal (SP)’ should be added for the ependymoma (EPN) and subependymoma (SubEPN). For an evidence-based review, the authors reviewed SCTs in the archives of the Seoul National University Hospital over the past decade. The frequent pathologies of primary IM-SCT were SP-EPN (45.1%), hemangioblastoma (20.0%), astrocytic tumors (17.4%, including pilocytic astrocytoma [4.6%] and diffuse midline glioma, H3 K27-altered [4.0%]), myxopapillary EPN (11.0%), and SP-subEPN (3.0%) in decreasing order. IDH-mutant astrocytomas, oligodendrogliomas, glioneuronal tumors, embryonal tumors, and germ cell tumors can occur but are extremely rare in the spinal cord. Genetic studies should support for the primary IM-SCT classification. In the 2021 WHO classifications, extramedullary SCT did not change significantly but contained several new genetically defined types of mesenchymal tumors. This article focused on primary IM-SCT for tumor frequency, age, sex difference, pathological features, and genetic abnormalities, based on a single-institute experience

Clinical Outcomes of Single-level Posterior Percutaneous Endoscopic Cervical Foraminotomy for Patients with Less Cervical Lordosis

Objective Posterior percutaneous endoscopic cervical foraminotomy (P-PECF) is a minimally invasive surgical technique for treatment of cervical radiculopathies. Application of P-PECF to patients with preexisting loss of cervical curvature (<10°) is still controversial because violation to facet joint may lead to kyphotic change. Clinical outcomes of P-PECF was analyzed and compared according to preoperative cervical curvature. Methods In this retrospective nested case-control study, 71 patients who underwent P-PECF due to foraminal soft disc herniation or bony stenosis were reviewed. P-PECF was performed by a single senior surgeon, and surgical methods were as previously described. Visual analogue pain scale on arm (Arm-VAS) was assessed preoperatively and postoperatively (1, 3, 6, 12 month and yearly thereafter). All patients were clinically followed for 24.5±20.0 months. The minimal clinically important difference of the Arm-VAS was set at 2.5. Patients with preoperative cervical curvature ≥10° were included in group I (n=32) and cervical curvature <10° or kyphosis were included in the group II (n=39). Results At the last follow up, 68/71 (96%) patients showed significant reduction of arm pain (Pre-operation, 7.4±2.0; post-operation, 1.5±2.0) after 1.74±0.29 months (95% CI; 1.18-2.31). The preoperative cervical curvature did not influence the outcome (p=0.4, T-test) and time to reach the clinical endpoint (p=0.34, Cox-logistic regression analysis). Conclusion P-PECF effectively reduced radicular pain due to foraminal soft disc herniation or stenosis. Preexisting loss of lordosis is not a risk factor for outcomes of P-PECF

High peak inspiratory pressure may be associated with intraoperative coughing during neurosurgery under general anesthesia without neuromuscular blockade: a retrospective study

Background The endotracheal cuff pressure depends on the airway pressure during positive-pressure ventilation. A high endotracheal cuff pressure may be related to intraoperative coughing, which can be detrimental during neurosurgery. We investigated the incidence of intraoperative coughing and its association with peak inspiratory pressure (PIP) during neurosurgery under general anesthesia without neuromuscular blockade. Methods This retrospective study divided 1656 neurosurgical patients who underwent total intravenous anesthesia without additional neuromuscular blockade after tracheal intubation into high (PIP > 21.6 cmH2O, n = 318) and low (PIP ≤ 21.6 cmH2O, n = 1338) PIP groups. After propensity score matching, 206 patients were selected in each group. Demographic, preoperative, surgical, and anesthetic data were collected retrospectively from electronic medical records and continuous ventilator, infusion pump, and bispectral index data from a data registry. Results Intraoperative coughing occurred in 30 (1.8%) patients, including 9 (0.5%) during the main surgical procedure. Intraoperative coughing was more frequent in the high PIP group than in the low PIP group before (14/318 [4.4%] vs. 16/1338 [1.2%], P < 0.001) and after (13/206 [6.3%] vs. 1/206 [0.5%], P = 0.003) propensity score matching. In multivariable logistic regression analysis after propensity score matching, a high PIP (odds ratio [95% confidence interval] 14.22 [1.81-111.73], P = 0.012), tidal volume divided by predicted body weight (mL/kg, 1.36 [1.09–1.69], P = 0.006), and surgical duration (min, 1.01 [1.00–1.01], P = 0.025) predicted intraoperative coughing. Conclusion The incidence of intraoperative coughing was 1.8% in neurosurgical patients undergoing general anesthesia without neuromuscular blockade and might be associated with a high PIP

Numerical Study on the Absorption Characteristics of Subwavelength Metallic Gratings Covered with a Lossy Dielectric Layer

Optical absorbers have been a topic of intense research due to their importance in many applications. In particular, multi-band and perfect absorption features in a desired frequency range are essential in broadband applications. In this work, we numerically studied the absorption properties of subwavelength metallic gratings coated with a dielectric layer. Here, the structure is considered to be an integration between a resonant cavity and a subwavelength metallic grating. Two appropriately designed structures can exhibit multi-band absorption properties. In addition to the numerical simulation results, we elaborate on determining the appropriate structural parameters that yield the desired spectral absorption profile in the visible range. We also numerically identify critical coupling conditions for perfect absorption

Longitudinal Change in Outcome of Frontal Lobe Epilepsy Surgery

BACKGROUND: Although the outcome of epilepsy surgery changes with time, few studies have considered longitudinal changes after frontal lobe epilepsy (FLE) surgery. OBJECTIVE: To assess the longitudinal changes after FLE surgery. METHODS: Resection of the seizure onset zone was performed in 76 patients with FLE. Invasive monitoring was performed in 56 of these 76. Awake craniotomy was performed in 43 of the 76 patients. More than 50% of patients were followed up for at least 7 years. The mean follow-up was 81 months. RESULTS: For all patients, the seizure-free rate was 79% at 6 months, 64% at 1 year, 55% at 2 years, and 55% at 7 years. For patients with cortical dysplasia, the seizure-free rate was 72% at 6 months, 53% at 1 year, 51% at 2 years, and 46% at 7 years. For patients with tumor, the seizure-free rate was 89% at 6 months, 83% at 1 year, 83% at 2 years, and 74% at 7 years. Patients with tumor showed better outcome than those with cortical dysplasia (P = .04). Although the overall seizure-free rate became stable after 2 years, individual status changed for up to 5 years. Seizures recurred in 11 patients within 1 year (early recurrence) and in 12 patients by 1 to 5 years (late recurrence). Antiepileptic drug (AED) medication was adjusted in all patients with recurrence. Patients with late recurrence had a more favorable response (Engel class I or II) than early recurrence (P < .01). CONCLUSION: The overall seizure outcome changes mostly during the first year. However, individual seizure status changes for up to 5 years. The outcome of late recurrence is favorable to AED adjustment.Elsharkawy AE, 2008, EPILEPSY RES, V81, P97, DOI 10.1016/j.eplepsyres.2008.04.017Lee JJ, 2008, SEIZURE-EUR J EPILEP, V17, P514, DOI 10.1016/j.seizure.2008.01.007Schramm J, 2008, NEUROSURGERY, V62, P463, DOI 10.1227/01.NEU.0000297106.65002.1FLee SY, 2008, SEIZURE-EUR J EPILEP, V17, P11, DOI 10.1016/j.seizure.2007.05.014Bonelli SB, 2007, EPILEPSIA, V48, P517, DOI 10.1111/j.1528-1167.2006.00943.xJeha LE, 2007, BRAIN, V130, P574, DOI 10.1093/brain/awl364Prevost J, 2006, EPILEPSIA, V47, P2198, DOI 10.1111/j.1528-1167.2006.00714.xYun CH, 2006, EPILEPSIA, V47, P574Lee SK, 2005, ANN NEUROL, V58, P525, DOI 10.1002/ana.20569Chung CK, 2005, EPILEPSIA, V46, P25Palmini A, 2004, NEUROLOGY, V62, pS2Kim CH, 2004, STEREOT FUNCT NEUROS, V82, P175, DOI 10.1159/000082206Hosking PG, 2003, SEIZURE-EUR J EPILEP, V12, P160, DOI 10.1016/S1059-1311(02)00189-9Lee DS, 2002, EUR J NUCL MED MOL I, V29, P607, DOI 10.1007/s00259-002-0774-9Kim YJ, 2002, YONSEI MED J, V43, P43Urbach H, 2002, EPILEPSIA, V43, P33Kim SK, 2001, EPILEPSIA, V42, P1531Lee SK, 2001, EPILEPSIA, V42, P850Kanemoto K, 2000, EPILEPSY RES, V39, P33Salanova V, 1996, BRAIN, V119, P989MISCHEL PS, 1995, J NEUROPATH EXP NEUR, V54, P137HENRY TR, 1993, J NUCL MED, V34, P1892ENGEL J, 1993, SURG TREATMENT EPILE, P553HENRY TR, 1991, EPILEPSY RES, V10, P174WINGKUN EC, 1991, EPILEPSIA, V32, P851

Evolution of spatial light modulator for high‐definition digital holography

Since the late 20th century, there has been rapid development in the display industry. Only 30 years ago, we used big cathode ray tube displays with poor resolution, but now most people use televisions or smartphones with very high‐quality displays. People now want images that are more realistic, beyond the two‐dimensional images that exist on the flat screen, and digital holography—one of the next‐generation displays—is expected to meet that need. The most important parameter that determines the performance of a digital hologram is the pixel pitch. The smaller the pixel pitch, the higher the level of hologram implementation possible. In this study, we fabricated the world‐smallest 3‐μm‐pixel‐pitch holographic backplane based on the spatial light modulator technology. This panel could display images with a viewing angle of more than 10°. Furthermore, a comparative study was conducted on the fabrication processes and the corresponding holographic results from the large to the small pixel‐pitch panels