ABC transporters as multidrug resistance mechanisms and the development of chemosensitizers for their reversal

One of the major problems related with anticancer chemotherapy is resistance against anticancer drugs. The ATP-binding cassette (ABC) transporters are a family of transporter proteins that are responsible for drug resistance and a low bioavailability of drugs by pumping a variety of drugs out cells at the expense of ATP hydrolysis. One strategy for reversal of the resistance of tumor cells expressing ABC transporters is combined use of anticancer drugs with chemosensitizers. In this review, the physiological functions and structures of ABC transporters, and the development of chemosensitizers are described focusing on well-known proteins including P-glycoprotein, multidrug resistance associated protein, and breast cancer resistance protein

Malakoplakia of the Kidney Extending to the Descending Colon in a Patient with Secondary Adrenal Insufficiency: A Case Report

Malakoplakia is an uncommon but distinctive type of chronic granulomatous inflammation that occurs most commonly in the genitourinary tract, especially the urinary bladder. Most patients have associated conditions characterized by some degree of immunosuppression, as seen in solid-organ transplants, autoimmune diseases requiring steroid use, chemotherapy, chronic systemic diseases, alcohol abuse and poorly controlled diabetes. We report an unusual case of the renal malakoplakia that involved the perirenal space, extending to the descending colon in a 65-year-old Korean woman with secondary adrenal insufficiency and diabetes mellitus

Infrared phases of 3D class R theories

We study the IR phases of 3D class R theories associated with closed non-hyperbolic 3-manifolds. Non-hyperbolic 3-manifolds can be obtained by performing Dehn fillings on 1-cusped hyperbolic 3-manifolds along exceptional slopes. In 3D-3D correspondence, the ‘exceptional’ Dehn filling corresponds to the gauging of an SU(2) flavor symmetry in a superconformal field theory associated with a 1-cusped 3-manifold with ‘small’ Chern-Simons levels. With several explicit examples, we analyze various interesting non-perturbative IR phenomena (such as spontaneous SUSY breaking, generation of mass gap and supersymmetry enhancement) from the ‘exceptional’ gaugings. Interestingly, distinguished features of the IR phases can be captured by simple topological properties of non-hyperbolic 3-manifolds. We also find that 3D class R theories associated with certain classes of atoroidal non-hyperbolic 3-manifolds always exhibit supersymmetry enhancement at low energy and actually flow to 3D rank-0 N= 4 SCFTs with trivial vacuum moduli space

Utilizing ECG Waveform Features as New Biometric Authentication Method

In this study, we are proposing a practical way for human identification based on a new biometric method. The new method is built on the use of the electrocardiogram (ECG) signal waveform features, which are produced from the process of acquiring electrical activities of the heart by using electrodes placed on the body. This process is launched over a period of time by using a recording device to read and store the ECG signal. On the contrary of other biometrics method like voice, fingerprint and iris scan, ECG signal cannot be copied or manipulated. The first operation for our system is to record a portion of 30 seconds out of whole ECG signal of a certain user in order to register it as user template in the system. Then the system will take 7 to 9 seconds in authenticating the template using template matching techniques. 44 subjects‟ raw ECG data were downloaded from Physionet website repository. We used a template matching technique for the authentication process and Linear SVM algorithm for the classification task. The accuracy rate was 97.2% for the authentication process and 98.6% for the classification task; with false acceptance rate 1.21%

Antimicrobial peptide from Bacillus subtilis CSB138: characterization, killing kinetics, and synergistic potency

We studied the prospect of synergy between the antimicrobial peptide p138c and non-peptide antibiotics for increasing the potency and bacterial killing kinetics of these agents. The production of p138c was maximized in the late exponential growth phase of Bacillus subtilis CSB138. Purification of p138c resulted in a total of 4800 arbitrary units (AU) with 19.15-fold and 3.2% recovery. Peptide p138c was thermo-tolerant up to 50 °C and stable at pH 5.8 to 11. The biochemical nature of p138c was determined by a bioassay, similar to tricine-SDS-PAGE, indicating inhibition at 3 kDa. The amino acid sequence of p138c was Gly-Leu-Glu-Glu-Thr-Val-Tyr-Ile-Tyr-Gly-Ala-Asn-Met-X-Ser. Potency and killing kinetics against vancomycin-resistant Staphylococcus aureus improved considerably when p138c was synergized with oxacillin, ampicillin, and penicillin G. The minimal inhibitory concentration (MIC) of p138c showed a 4-, 8-, and 16-fold improvement when p138c was combined with oxacillin, ampicillin, and penicillin G, respectively. The fractional inhibitory concentration index for the combination of p138c and oxacillin, ampicillin, and penicillin G was 0.3125, 0.25, and 0.09, respectively. Synergy with non-peptide antibiotics resulted in enhanced killing kinetics of p138c. Hence, the synergy between antimicrobial peptide and non-peptide antibiotics may enhance the potency and bacterial killing kinetics, providing more potent and rapidly acting agents for therapeutic use. [Int Microbiol 20(1):43-53 (2017)]Keywords: Bacillus subtilis · antimicrobial peptides · killing kinetic