10 research outputs found

    The Student Movement Volume 106 Issue 8: Cardinals Cheer, Thanksgiving is Here!

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    HUMANS Meet Your 2021-2022 AU Cardinals Men\u27s Basketball Team, Interviewed by: Timmy Duado What Are You Thankful For?, Interviewed by: Grace No Meet Your 2021-2022 AU Cardinals Women\u27s Basketball Team, Interviewed by: Taylor Uphus ARTS & ENTERTAINMENT Thanksgiving Film Recommendations!, Megan Napod The Harder They Fall , Hannah Cruse What is CATHARSIS?, Solana Campbell NEWS Andrews Autumn Conference on Science & Religion, Abigail Lee AUSA Hosts Open Gym, Karenna Lee Campus Concert Crawl, Abigail Lee IDEAS Hidden out of Season, Evin-Nazya Musgrove Risk and Reward in Squid Game , Yoel Kim The Necessity of Firearm Safety Education, Nathan Cheng PULSE Honors Testimony: Worship in the Church, Honors Student Productivity... (and Pronouns ), T Bruggemann Thanksgiving Traditions of Your Student Movement Editors, Alannah Tjhatra THE LAST WORD Thanksgiving Dinner and Communion, Alyssa Henriquezhttps://digitalcommons.andrews.edu/sm-106/1007/thumbnail.jp

    Robust estimation of bacterial cell count from optical density

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    Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

    Automated Navigation and Mobile Vehicle Control using Wireless Sensor Network Technology

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    This paper introduces a novel concept for automated navigation and control of a mobile platform. It utilizes an ad-hoc mobile wireless sensor network to provide navigational information to the mobile platform embedded control system. The embedded controller is realized with an ARM9 SOC based single board computer, with real time, multi-thread software implementation using a real time Linux operating system framwork. We have repeatedly tested the automated navigation and control concept on a wheel based mobile platform in an indoor environment, and found the performance meeting our original design goals

    A single dose of K11777 protects chicken embryos in an <i>in vivo</i> model of toxoplasmosis.

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    <p>Fourteen day old chick eggs (N = 10/ treatment) were injected through the chorioallantoic vein with 10<sup>4</sup> RH tachyzoites in media alone (Control) or media containing K11777 for a final blood concentration of 20 uM). The Kaplan-Meier Survival curve of the chick embryos (N = 10 per group) showed that 100% of the chick embryos treated with a single dose of K11777 embryos survived until Day 6 vs. only 20% of the control embryos (p = 0.015 by Cox regression analysis).</p

    Two key cathepsins, TgCPB and TgCPL, are targeted by the vinyl sulfone inhibitor K11777 in <i>in vitro</i> and <i>in vivo</i> models of toxoplasmosis - Fig 3

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    <p>(A) A single dose of K11777 reduces parasite load in the brain of chicken embryos. K11777 treated chicken embryos (N = 10) (final blood concentration 20 μM) showed a 3-log reduction in parasite burden in brain tissue as measured by quantitative PCR compared to chicken embryos injected with parasites in media alone (N = 10) (Control). Error bars reflect SEM. (B) A single dose of K11777 reduces parasite load in the liver of chick embryos. K11777 treated chicken embryos (final blood concentration 20 μM) showed a 4-log reduction in parasite burden in liver tissue as measured by quantitative PCR compared to chicken embryos injected with parasites in media alone (Control). Error bars reflect SEM.</p

    Two key cathepsins, TgCPB and TgCPL, are targeted by the vinyl sulfone inhibitor K11777 in <i>in vitro</i> and <i>in vivo</i> models of toxoplasmosis

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    <div><p>Although toxoplasmosis is one of the most common parasitic infections worldwide, therapeutic options remain limited. Cathepsins, proteases that play key roles in the pathogenesis of toxoplasmosis and many other protozoan infections, are important potential therapeutic targets. Because both TgCPB and TgCPL play a role in <i>T</i>. <i>gondii</i> invasion, we evaluated the efficacy of the potent, irreversible vinyl sulfone inhibitor, K11777 (<i>N-</i>methyl-piperazine-Phe-homoPhe-vinylsulfone-phenyl). The inhibitor’s toxicity and pharmacokinetic profile have been well-studied because of its <i>in vitro</i> and <i>in vivo</i> activity against a number of parasites. We found that it inhibited both TgCPB (EC50 = 114 nM) and TgCPL (EC50 = 71 nM) <i>in vitro</i>. K11777 also inhibited invasion of human fibroblasts by RH tachyzoites by 71% (p = 0.003) and intracellular replication by >99% (p<0.0001). <i>In vivo</i>, a single dose of K11777 led to 100% survival of chicken embryos in an model of acute toxoplasmosis (<i>p</i> = 0.015 Cox regression analysis). Therefore, K11777 shows promise as a novel therapeutic agent in the treatment of toxoplasmosis, and may prove to be a broadly effective anti-parasitic agent.</p></div
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