Identification of precipitation onset based on Cloudsat observations

AbstractObservations of cloud vertical structure by Cloud Profiling Radar on CloudSat satellite provide a unique opportunity to globally identify the onset of precipitation. In this study, based on a conceptual model for an adiabatic cloud, a new method to determine the onset of precipitation in marine warm clouds is developed. The new method uses the slope of radar reflectivities near the cloud top, which gradually reverses its signs as drizzle occurs. By analyzing multiyear CloudSat data, it is found that globally the radar reflectivity threshold for precipitation onset varies from −18 to −13dBZ with an average value of −16dBZ. The corresponding liquid water path threshold for precipitation onset is also studied by analyzing satellite microwave observations collocated with CloudSat data. Results show that the liquid water path threshold is 190gm−2 as a global mean, varying from 150 to over 300gm−2 depending on regions

Historical reconstructions of water quality in the Kimberley using sediment records. Report of 2.2.9 prepared for the Kimberley Marine Research Program

The Kimberley Marine Research Project 2.2.9 made use of a suite of palaeoecological approaches to reconstruct a chronology of change in coastal water quality over the last approximately 100 years. The biogeochemical proxies addressed phytoplankton composition and biomass, temperature and terrestrial influences. Where possible these were matched to historical land/water use, meteorological or hydrological observational records. The project examined sediment cores from three coastal locations in the Kimberley Region of Western Australia, Koolama Bay (King George River), Cygnet Bay and Roebuck Bay. Each sampling location provided a contrast with which to evaluate changes over either a spatial or temporal gradient of human or natural influences

Anthropogenic impacts on hyperbenthos in the coastal waters of Sishili Bay, Yellow Sea

A survey on the hyperbenthic communities was carried out in the coastal waters of Sishili Bay in the Yellow Sea in July 2009, to investigate the impact of putative anthropogenic activities related to the presence of a sewage outfall, a harbor and an aquaculture site on the benthic ecosystem. An Agassiz net trawl was used to collect hyperbenthos at 10 sampling stations. Species, diversity, abundance, and biomass were analyzed against water sample data and historical data obtained from records from nearby Yantai City. Fifty-two species were identified in the region, of which Crustacea were the most abundant taxon, followed by Echinodermata. Dominant species included five crustaceans, two echinoderms, one mollusk and one fish species. The results of a BIOENV analysis show that the concentrations of NO3-N and Chl-a were slightly positively correlated with hyperbenthic community structure, while other factors were negatively correlated, including sediment grain size and the percentage of TN and TOC in sediment. Abundance-Biomass Comparison (ABC method) curves indicate that the hyperbenthos in Sishili Bay had been disturbed by putative sources of human activities. Eight out of 10 stations were classified as "moderately perturbed" to "perturbed"

Production of Recombinant Human DNA Polymerase Delta in a Bombyx mori Bioreactor

Eukaryotic DNA polymerase δ (pol δ) plays a crucial role in chromosomal DNA replication and various DNA repair processes. It is thought to consist of p125, p66 (p68), p50 and p12 subunits. However, rigorous isolation of mammalian pol δ from natural sources has usually yielded two-subunit preparations containing only p125 and p50 polypeptides. While recombinant pol δ isolated from infected insect cells have some problems of consistency in the quality of the preparations, and the yields are much lower. To address these deficiencies, we have constructed recombinant BmNPV baculoviruses using MultiBac system. This method makes the generation of recombinant forms of pol δ containing mutations in any one of the subunits or combinations thereof extremely facile. From about 350 infected larvae, we obtained as much as 4 mg of pol δ four-subunit complex. Highly purified enzyme behaved like the one of native form by rigorous characterization and comparison of its activities on poly(dA)/oligo(dT) template-primer and singly primed M13 DNA, and its homogeneity on FPLC gel filtration. In vitro base excision repair (BER) assays showed that pol δ plays a significant role in uracil-intiated BER and is more likely to mediate LP BER, while the trimer lacking p12 is more likely to mediate SN BER. It seems likely that loss of p12 modulates the rate of SN BER and LP BER during the repair process. Thus, this work provides a simple, fast, reliable and economic way for the large-scale production of human DNA polymerase δ with a high activity and purity, setting up a new platform for our further research on the biochemical properties of pol δ, its regulation and the integration of its functions, and how alterations in pol δ function could contribute to the etiology of human cancer or other diseases that can result from loss of genomic stability

P50, the Small Subunit of DNA Polymerase Delta, Is Required for Mediation of the Interaction of Polymerase Delta Subassemblies with PCNA

Mammalian DNA polymerase δ (pol δ), a four-subunit enzyme, plays a crucial and versatile role in DNA replication and various DNA repair processes. Its function as a chromosomal DNA polymerase is dependent on the association with proliferating cell nuclear antigen (PCNA) which functions as a molecular sliding clamp. All four of the pol δ subunits (p125, p50, p68, and p12) have been reported to bind to PCNA. However, the identity of the subunit of pol δ that directly interacts with PCNA and is therefore primarily responsible for the processivity of the enzyme still remains controversial. Previous model for the network of protein-protein interactions of the pol δ-PCNA complex showed that pol δ might be able to interact with a single molecule of PCNA homotrimer through its three subunits, p125, p68, and p12 in which the p50 was not included in. Here, we have confirmed that the small subunit p50 of human pol δ truthfully interacts with PCNA by the use of far-Western analysis, quantitative ELISA assay, and subcellular co-localization. P50 is required for mediation of the interaction between pol δ subassemblies and PCNA homotrimer. Thus, pol δ interacts with PCNA via its four subunits

Corrosion Activity on CFRP-Strengthened RC Piles of High-Pile Wharf in a Simulated Marine Environment

We report test results from an experimental study to investigate the effectiveness of carbon fiber-reinforced polymer (CFRP) against reinforcing steel bar corrosion. Twelve reinforced-concrete pile specimens of 180 mm square by 1,600 mm long were cast. Three pile specimens were corroded to 5% steel mass loss and then strengthened with CFRP sheets; four specimens were strengthened by using CFRP sheets, whereas the remaining five specimens were not strengthened. The specimens were placed in a simulated marine environment, and corrosion was induced by an impressed current technique. At different theoretical corrosion degrees, nondestructive tests were performed to investigate the corrosion activity of the pile specimens, and destructive tests were performed to determine reinforcing steel bar mass loss. Based on the findings, the effectiveness of the CFRP-strengthened RC piles under aggressive marine environmental conditions was established