1,638 research outputs found
Isolation of 39 polymorphic microsatellite loci and the development of a fluorescently labelled marker set for the Eurasian badger
We have isolated 78 microsatellite loci from the Eurasian badger (Meles meles). Of the 52 loci characterized, 39 were found to be polymorphic. A fluorescently labelled primer set was developed to enable individual-specific 17-locus genotypes to be obtained efficiently
Estimation of badger abundance using faecal DNA typing
1.Wildlife management and conservation programmes often require accurate information
on population density, but this can be difficult to obtain, particularly when the species in question is nocturnal or cryptic. Badger populations in Britain are of intense management interest because they are a wildlife reservoir host of bovine tuberculosis (TB). Attempts to manage this infection in badgers, whether by population control or vaccination, require reliable methods of estimating population size. In addition, such estimates are also required to support research into badger ecology and TB epidemiology. Currently, the most accurate estimates of local badger population size are
obtained from labour-intensive and time-consuming markârecapture studies. 2. In recent years, DNA has been successfully extracted from the faeces of certain mammals,
and used to generate a genetic profile of the defecating individual. Here we report on an application of this technology to estimate badger abundance.3.Faecal samples were collected on 10 consecutive days from every freshly deposited dropping at latrine sites close to occupied setts in three badger social groups. Badger
DNA was extracted from 89% of samples, and 20 different individuals were reliably identified. The genotypes derived from the faecal samples were compared with those
obtained from blood or samples from badgers live trapped at the same setts.4.The faecal genotypes from badgers with known trap histories revealed that latrines were used equally by males and females, and by badgers ranging in age from cubs(< 1 year old) to 9 years old. Individual badgers used the latrines on between one and six different nights. Rarefaction analysis produced abundance estimates that closely matched those obtained from live trapping.
5.Synthesis and applications. Systematic sampling and genetic typing of fresh faeces from badger latrines can provide data that can be used to estimate abundance accurately.This approach requires considerably less human resources than repeated live trapping
and markârecapture. The technique may be valuable for future badger research and management in relation to bovine TB, where accurate estimates of abundance at a local
scale are required
Single-Step Quantum Search Using Problem Structure
The structure of satisfiability problems is used to improve search algorithms
for quantum computers and reduce their required coherence times by using only a
single coherent evaluation of problem properties. The structure of random k-SAT
allows determining the asymptotic average behavior of these algorithms, showing
they improve on quantum algorithms, such as amplitude amplification, that
ignore detailed problem structure but remain exponential for hard problem
instances. Compared to good classical methods, the algorithm performs better,
on average, for weakly and highly constrained problems but worse for hard
cases. The analytic techniques introduced here also apply to other quantum
algorithms, supplementing the limited evaluation possible with classical
simulations and showing how quantum computing can use ensemble properties of NP
search problems.Comment: 39 pages, 12 figures. Revision describes further improvement with
multiple steps (section 7). See also
http://www.parc.xerox.com/dynamics/www/quantum.htm
Effect of tartaric acid and phosphoric acid on the water resistance of magnesium oxychloride (MOC) cement
Magnesium oxychloride (MOC) cement is featured with high early strength, low thermal conductivity and low density, but is not widely applied in construction engineering due to its poor water resistance capability. This research has studied the effect of phosphoric acid and tartaric acid additions on the water resistance of MOC cement pastes, in which also reports the effects on setting time, hydration reactions, compressive strength, phase composition, thermal stability and microstructure. 1 wt% of phosphoric acid and tartaric acid additions can improve the water resistance and reduce thermal stability of MOC cement pastes, which is associated with formation of gel-like 5Mg(OH) 2 ·MgCl 2 ·8H 2 O. Moreover, these additions reduce the compressive strength and prolong the setting time of MOC cement pastes, as well as increase the total porosity, the volume fraction of gel pores (100 nm), however, decrease the volume fraction of small capillary pores (10â100 nm) of MOC cement pastes. These effects are caused by both additives but are most pronounced for MOC cement pastes containing phosphoric acid. In addition, 2Mg(OH) 2 ·MgCl 2 ·2H 2 O is a transitional phase in the formative stage of 5Mg(OH) 2 ·MgCl 2 ·8H 2 O in MOC cement
Control of drying shrinkage of magnesium silicate hydrate gel cements
Cracks were observed when the magnesium silicate hydrate gel cement (prepared by 40% MgO/60% silica fume) was dried. This drying cracking is believed to be caused when unbound water evaporates from the binder. The shrinkage upon forced drying to 200 °C of mortars made up from a reactive magnesium oxide, silica fume and sand was measured using dilatometry. The magnitude of the drying shrinkage was found to decrease when more sand or less water was added to the mortars and can be as low as 0.16% for a mortar containing 60 wt% sand and a water to cement ratio of 0.5, which is of a similar order of magnitude as observed in Portland cement based mortars and concretes. A simple geometrical interpretation based on packing of the particles in the mortar can explain the observed drying shrinkages and based on this analysis the drying shrinkage of the hydration products at zero added solid is estimated to be 7.3% after 7 days of curing
Extremal Optimization at the Phase Transition of the 3-Coloring Problem
We investigate the phase transition of the 3-coloring problem on random
graphs, using the extremal optimization heuristic. 3-coloring is among the
hardest combinatorial optimization problems and is closely related to a 3-state
anti-ferromagnetic Potts model. Like many other such optimization problems, it
has been shown to exhibit a phase transition in its ground state behavior under
variation of a system parameter: the graph's mean vertex degree. This phase
transition is often associated with the instances of highest complexity. We use
extremal optimization to measure the ground state cost and the ``backbone'', an
order parameter related to ground state overlap, averaged over a large number
of instances near the transition for random graphs of size up to 512. For
graphs up to this size, benchmarks show that extremal optimization reaches
ground states and explores a sufficient number of them to give the correct
backbone value after about update steps. Finite size scaling gives
a critical mean degree value . Furthermore, the
exploration of the degenerate ground states indicates that the backbone order
parameter, measuring the constrainedness of the problem, exhibits a first-order
phase transition.Comment: RevTex4, 8 pages, 4 postscript figures, related information available
at http://www.physics.emory.edu/faculty/boettcher
Eulerian simulation of the fluid dynamics of helicopter brownout
A computational model is presented that can be used to simulate the development of the dust cloud
that can be entrained into the air when a helicopter is operated close to the ground in desert or dusty
conditions. The physics of this problem, and the associated pathological condition known as âbrownoutâ
where the pilot loses situational awareness as a result of his vision being occluded by dust suspended in the
flow around the helicopter, is acknowledged to be very complex. The approach advocated here involves
an approximation to the full dynamics of the coupled particulate-air system. Away from the ground, the
model assumes that the suspended particles remain in near equilibrium under the action of aerodynamic
forces. Close to the ground, this model is replaced by an algebraic sublayer model for the saltation and
entrainment process. The origin of the model in the statistical mechanics of a distribution of particles
governed by aerodynamic forces allows the validity of the method to be evaluated in context by comparing
the physical properties of the suspended particulates to the local properties of the flow field surrounding
the helicopter. The model applies in the Eulerian frame of reference of most conventional Computational
Fluid Dynamics codes and has been coupled with Brownâs Vorticity Transport Model. Verification of the
predictions of the coupled model against experimental data for particulate entrainment and transport in
the flow around a model rotor are encouraging. An application of the coupled model to analyzing the
differences in the geometry and extent of the dust clouds that are produced by single main rotor and
tandem-rotor configurations as they decelerate to land has shown that the location of the ground vortex
and the size of any regions of recirculatory flow, should they exist, play a primary role in governing the
extent of the dust cloud that is created by the helicopter
Developing circular concrete: Acid treatment of waste concrete fines
The development of circular concrete, to enable key components to be extracted and reused, is a key requirement to achieve sustainability in the built environment. Current industry practice for end-of-life concrete is best described as down-cycling because recycled concrete aggregate has limited use, with disposal of the associated crushed concrete fines. Acid treatment of waste concrete is being investigated to allow key concrete components to become circular and, in this work, the effect of acetic acid concentration, liquid/solid (L/S) ratio, reaction time and temperature on the leaching of waste concrete fines is reported. An acid concentration of 0.6 mol/L, an L/S ratio of 7 ml/g, and a reaction time of 6 h at ambient temperature allows clean sand to be extracted from concrete fines. This performs identically to new sand in mortar samples. We show for the first time that the dried and ground silica-rich residue produced by acid leaching has pozzolanic properties comparable to commercially available supplementary cementitious materials (SCM) such as blast furnace slag and coal fly ash. The potential for CO2 sequestration using the Ca2+-rich leached solution to form CaCO3 is calculated. The research shows that acid leaching of concrete fines can produce clean reusable sand, generates a viable SCM and sequester significant amounts of CO2 by forming precipitated calcium carbonate
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