Étude bactériologique comparative des fromages frais marocains commercialisés (Mahlabats) et des fromages fabriqués au laboratoire

Vingt échantillons de fromage frais traditionnel marocain (jben) fabriqué à partir du lait cru, prélevés de cinq laiteries traditionnelles (Mahlabats) de la ville de Kenitra ;ont été soumis à des analyses microbiologiques, à fin de mettre en évidence leur qualité bactériologique et de les comparer avec le fromage contrôlé préparé au laboratoire . Les résultats obtenus montrent que le pH moyen de ces échantillons commercialisés est de 4,25 et l'acidité moyenne est de 87,4D°. Ces valeurs sont respectivement de l’ordre de 4,18 et 83D au niveau des fromages contrôlés (10 échantillons). La charge microbienne aérobie totale est en moyenne de 1,14 107UFC/g, alors que pour les fromages contrôlés les valeurs oscillent entre 3 et 9.106UFC/g. La flore d'origine fécale (coliformes totaux et fécaux) est plus importante dans les fromages commercialisés et en moyenne respectives de 1,04.103 UFC/g et 5,7.104 UFC/g. Les fromages contrôlés sont dépourvus de toute contamination d’origine fécale. Nous avons souligné également l’absence totale des Staphylocoques, Salmonelles et clolésstriduims dans tous les échantillons analysés.Mots-clés : fromage frais, qualité bactériologique

Evaluation Du Système D’hygiène Dans Les Locaux Des Restaurationsscolaires, À La Région d’El Gharb Cherardabnihssen, Par Rapport Auxexigences Normatives Internationales

The implementation of hygiene system in the school catering is based on the application of good hygienic practice and regulations of food safety (security). These measures of hygiene contribute absolutely, in the elimination of the potential food risks susceptible to be at the origin of several diseases. This article presents the results of a study carried out to evaluate the degree of the respect for the sanitary conditions at the level of three school restaurants. The result of our study aims to determine the level of compliance of their hygienic systems to international hygienic standards. The rates of conformity of the sites of the study were of the order of 33, 60 %, 33, 00 % and of 48, 58 % respectively at the level of the establishment n°1, n°2 and n°3. The recorded results show apparently, the level of conformity with regard to the standards. In front of this situation, it is essential to plan and to implement relevant actions of improvement and to engage ways and resources to eliminate the noticed gaps and increase the level of conformit

Dual Infection and Superinfection Inhibition of Epithelial Skin Cells by Two Alphaherpesviruses Co-Occur in the Natural Host

Hosts can be infected with multiple herpesviruses, known as superinfection; however, superinfection of cells is rare due to the phenomenon known as superinfection inhibition. It is believed that dual infection of cells occurs in nature, based on studies examining genetic exchange between homologous alphaherpesviruses in the host, but to date, this has not been directly shown in a natural model. In this report, gallid herpesvirus 2 (GaHV-2), better known as Marek’s disease virus (MDV), was used in its natural host, the chicken, to determine whether two homologous alphaherpesviruses can infect the same cells in vivo. MDV shares close similarities with the human alphaherpesvirus, varicella zoster virus (VZV), with respect to replication in the skin and exit from the host. Recombinant MDVs were generated that express either the enhanced GFP (eGFP) or monomeric RFP (mRFP) fused to the UL47 (VP13/14) herpesvirus tegument protein. These viruses exhibited no alteration in pathogenic potential and expressed abundant UL47-eGFP or -mRFP in feather follicle epithelial cells in vivo. Using laser scanning confocal microscopy, it was evident that these two similar, but distinguishable, viruses were able to replicate within the same cells of their natural host. Evidence of superinfection inhibition was also observed. These results have important implications for two reasons. First, these results show that during natural infection, both dual infection of cells and superinfection inhibition can co-occur at the cellular level. Secondly, vaccination against MDV with homologous alphaherpesvirus like attenuated GaHV-2, or non-oncogenic GaHV-3 or meleagrid herpesvirus (MeHV-1) has driven the virus to greater virulence and these results implicate the potential for genetic exchange between homologous avian alphaherpesviruses that could drive increased virulence. Because the live attenuated varicella vaccine is currently being administered to children, who in turn could be superinfected by wild-type VZV, this could potentiate recombination events of VZV as well

Late Holocene flood magnitudes in the Lower Rhine river valley and upper delta resolved by a two‐dimensional hydraulic modelling approach

Palaeoflood hydraulic modelling is essential for quantifying ‘millennial flood’ events not covered in the instrumental record. Palaeoflood modelling research has largely focused on one‐dimensional analysis for geomorphologically stable fluvial settings because two‐dimensional analysis for dynamic alluvial settings is time consuming and requires a detailed representation of the past landscape. In this study, we make the step to spatially continuous palaeoflood modelling for a large and dynamic lowland area. We applied advanced hydraulic model simulations (1D–2D coupled set‐up in HEC‐RAS with 950 channel sections and 108 × 103 floodplain grid cells) to quantify the extent and magnitude of past floods in the Lower Rhine river valley and upper delta. As input, we used a high‐resolution terrain reconstruction (palaeo‐DEM) of the area in early mediaeval times, complemented with hydraulic roughness values. After conducting a series of model runs with increasing discharge magnitudes at the upstream boundary, we compared the simulated flood water levels with an inventory of exceeded and non‐exceeded elevations extracted from various geological, archaeological and historical sources. This comparison demonstrated a Lower Rhine millennial flood magnitude of approximately 14,000 m3/s for the Late Holocene period before late mediaeval times. This value exceeds the largest measured discharges in the instrumental record, but not the design discharges currently accounted for in flood risk management