Age related histological and histochemical studies on the testis of Aseel and Vanaraja breeds of poultry

Birds (10) of 2 age group, viz. 5 months (grower) and 13 months (adult) of Aseel and Vanaraja breeds of poultry were used. The thickness of tunica albuginea, maximum and minimum diameter of seminiferous tubules, diameter of primary spermatocytes and number of sustentacular cells or Sertoli cells per seminiferous tubule were significantly higher in growers and adults of Vanaraja than Aseel whereas, number of interstitial cells per field were significantly higher in growers and adults of Aseel than Vanaraja. The density of connective tissue fibers, PAS activity and AB-PAS activity was more in both groups of Vanaraja than Aseel

Second release of the CoRe database of binary neutron star merger waveforms

We present the second data release of gravitational waveforms from binaryneutron star merger simulations performed by the Computational Relativity(CoRe) collaboration. The current database consists of 254 different binaryneutron star configurations and a total of 590 individual numerical-relativitysimulations using various grid resolutions. The released waveform data containthe strain and the Weyl curvature multipoles up to $\ell=m=4$. They span asignificant portion of the mass, mass-ratio,spin and eccentricity parameterspace and include targeted configurations to the events GW170817 and GW190425.CoRe simulations are performed with 18 different equations of state, seven ofwhich are finite temperature models, and three of which account fornon-hadronic degrees of freedom. About half of the released data are computedwith high-order hydrodynamics schemes for tens of orbits to merger; the otherhalf is computed with advanced microphysics. We showcase a standard waveformerror analysis and discuss the accuracy of the database in terms offaithfulness. We present ready-to-use fitting formulas for equation ofstate-insensitive relations at merger (e.g. merger frequency), luminosity peak,and post-merger spectrum.<br

Exploration of two methods for quantitative Mitomycin C measurement in tumor tissue in vitro and in vivo

Two methods of quantifying Mitomycin C in tumor tissue are explored. A method of ultraviolet-visible absorption microscopy is developed and applied to measure the concentration of Mitomycin C in preserved mouse tumor tissue, as well as in gelatin samples. Concentrations as low as 60 μM can be resolved using this technique in samples that do not strongly scatter light. A novel method for monitoring the Mitomycin C concentrations inside a tumor is developed, based on microdialysis and ultraviolet-visible spectroscopy. A pump is used to perfuse a microdialysis probe with Ringer’s solution, which is fed to a flow cell to determine intratumor concentrations in real time to within a few μM. The success and limitations of these techniques are identified, and suggestions are made as to further development. To the authors’ knowledge these are the first attempts made to quantify Mitomycin C concentrations in tumor tissue

Capture, Reconstruction, and Representation of the Visual Real World for Virtual Reality

We provide an overview of the concerns, current practice, and limitations for capturing, reconstructing, and representing the real world visually within virtual reality. Given that our goals are to capture, transmit, and depict complex real-world phenomena to humans, these challenges cover the opto-electro-mechanical, computational, informational, and perceptual fields. Practically producing a system for real-world VR capture requires navigating a complex design space and pushing the state of the art in each of these areas. As such, we outline several promising directions for future work to improve the quality and flexibility of real-world VR capture systems

Application of prolonged microdialysis sampling in carboplatin-treated cancer patients

Purpose: To better understand the mechanisms underlying (in)sensitivity of tumors to anticancer drugs, assessing intra-tumor drug pharmacokinetics (PKs) could be important. We explored the feasibility of microdialysis in tumor tissue for multiple days in a clinical setting, using carboplatin as model drug. Methods: Plasma and microdialysate samples from tumor and adipose normal tissues were collected up to 47 h after dosing in eight carboplatin-treated patients with an accessible (sub)cutaneous tumor. Results: Pharmacokinetics were evaluable in tumor tissue in 6/8 patients and in adipose normal tissue in 3/8 patients. Concentration-time curves of unbound platinum in both the tissues followed the pattern of the curves in plasma, with exposure ratios of tissue versus plasma ranging from 0.64 to 1.46. Conclusions: Microdialysis can be successfully employed in ambulant patients for multiple days, which enables one to study tissue PK of anticancer drugs in normal and malignant tissues in more detail

Maize for Changing Climate - Chasing the Moving Target

The average annual growth rate of harvested maize area from 1993 to 2013 was 2.7% in Africa, 3.1% in Asia, and 4.6% in Latin America (FAOSTAT, 2018). Maize has emerged as the cereal with largest global production, which surpassed rice in 1996 and wheat in 1997, and its production is increasing at twice the annual rate of rice and three times that of wheat (Fischer et al., 2014). Among cereals, including rice, wheat and other coarse cereal, maize has recorded highest increase in area and productivity during 2006-2015 and is projected to keep the momentum during 2016-2025 (OECD/FAO, 2016). Asia, with its 31% share in global maize production from about 34.0% of the total global area harvested, is the second largest maize producer in the world. The current decade continued impressive growth in maize production, as all the sub-regions showed significant increase in maize production (Figure 1), including Southeast Asia -10.8%, Southern Asia - 27.3% and East Asia - 30.6%, which resulted in an overall 27.7% maize production increase in Asia within a short period of 2010-2016 (FAOSTAT, 2018). These gains in maize production were contributed by increase in productivity per unit area and increase in maize growing areas in some countries

Protandim, a Fundamentally New Antioxidant Approach in Chemoprevention Using Mouse Two-Stage Skin Carcinogenesis as a Model

Oxidative stress is an important contributor to cancer development. Consistent with that, antioxidant enzymes have been demonstrated to suppress tumorigenesis when being elevated both in vitro and in vivo, making induction of these enzymes a more potent approach for cancer prevention. Protandim, a well-defined combination of widely studied medicinal plants, has been shown to induce superoxide dismutase (SOD) and catalase activities and reduce superoxide generation and lipid peroxidation in healthy human subjects. To investigate whether Protandim can suppress tumor formation by a dietary approach, a two-stage mouse skin carcinogenesis study was performed. At the end of the study, the mice on a Protandim-containing basal diet had similar body weight compared with those on the basal diet, which indicated no overt toxicity by Protandim. After three weeks on the diets, there was a significant increase in the expression levels of SOD and catalase, in addition to the increases in SOD activities. Importantly, at the end of the carcinogenesis study, both skin tumor incidence and multiplicity were reduced in the mice on the Protandim diet by 33% and 57% respectively, compared with those on basal diet. Biochemical and histological studies revealed that the Protandim diet suppressed tumor promoter-induced oxidative stress (evidenced by reduction of protein carbonyl levels), cell proliferation (evidenced by reduction of skin hyperplasia and suppression of PKC/JNK/Jun pathway), and inflammation (evidenced by reduction of ICAM-1/VCAM-1 expression, NF-κB binding activity, and nuclear p65/p50 levels). Overall, induction of antioxidant enzymes by Protandim may serve as a practical and potent approach for cancer prevention

Global Perspectives on Task Shifting and Task Sharing in Neurosurgery.

BACKGROUND: Neurosurgical task shifting and task sharing (TS/S), delegating clinical care to non-neurosurgeons, is ongoing in many hospital systems in which neurosurgeons are scarce. Although TS/S can increase access to treatment, it remains highly controversial. This survey investigated perceptions of neurosurgical TS/S to elucidate whether it is a permissible temporary solution to the global workforce deficit. METHODS: The survey was distributed to a convenience sample of individuals providing neurosurgical care. A digital survey link was distributed through electronic mailing lists of continental neurosurgical societies and various collectives, conference announcements, and social media platforms (July 2018-January 2019). Data were analyzed by descriptive statistics and univariate regression of Likert Scale scores. RESULTS: Survey respondents represented 105 of 194 World Health Organization member countries (54.1%; 391 respondents, 162 from high-income countries and 229 from low- and middle-income countries [LMICs]). The most agreed on statement was that task sharing is preferred to task shifting. There was broad consensus that both task shifting and task sharing should require competency-based evaluation, standardized training endorsed by governing organizations, and maintenance of certification. When perspectives were stratified by income class, LMICs were significantly more likely to agree that task shifting is professionally disruptive to traditional training, task sharing should be a priority where human resources are scarce, and to call for additional TS/S regulation, such as certification and formal consultation with a neurosurgeon (in person or electronic/telemedicine). CONCLUSIONS: Both LMIC and high-income countries agreed that task sharing should be prioritized over task shifting and that additional recommendations and regulations could enhance care. These data invite future discussions on policy and training programs

Postsynaptic nigrostriatal dopamine receptors and their role in movement regulation

The article presents the hypothesis that nigrostriatal dopamine may regulate movement by modulation of tone and contraction in skeletal muscles through a concentration-dependent influence on the postsynaptic D1 and D2 receptors on the follow manner: nigrostriatal axons innervate both receptor types within the striatal locus somatotopically responsible for motor control in agonist/antagonist muscle pair around a given joint. D1 receptors interact with lower and D2 receptors with higher dopamine concentrations. Synaptic dopamine concentration increases immediately before movement starts. We hypothesize that increasing dopamine concentrations stimulate first the D1 receptors and reduce muscle tone in the antagonist muscle and than stimulate D2 receptors and induce contraction in the agonist muscle. The preceded muscle tone reduction in the antagonist muscle eases the efficient contraction of the agonist. Our hypothesis is applicable for an explanation of physiological movement regulation, different forms of movement pathology and therapeutic drug effects. Further, this hypothesis provides a theoretical basis for experimental investigation of dopaminergic motor control and development of new strategies for treatment of movement disorders