Comparison of the effect for fracture resistance of different coronally extended post length with two different post materials

Aims and Objectives: To compare the effect for fracture resistance of different coronally extended post length with two different post materials. Materials and Methods: One hundred and sixty endodontically treated maxillary central incisors embedded in acrylic resin with decoronated root portion were taken for the study. The postspaces were prepared according to standard protocol. The samples were divided into two groups according to the post material: glass-fiber post and Quartz fiber post. These groups were further subdivided on the basis of coronal extension of 4 and 6 mm for glass fiber and Quartz fiber posts, respectively. The posts were then luted with dual-polymerizing resin cement followed by core buildup. Samples were subjected to increasing compressive oblique load until fracture occurred in a universal testing machine. Data were analyzed with one-way ANOVA and independent Student's t-test. Analysis was done using SPSS version 15 (SPSS Inc., Chicago, IL, USA) Windows software program. Results: Glass fiber post with coronal extension of 4 mm (182.8 N) showed better results than with 6-mm length (124.1 N). Similarly, in quartz fiber posts group, 4-mm postlength (314 N) was better when compared with 6 mm (160 N). The 4-mm coronal extension of quartz fiber post displayed superior fracture resistance. Conclusions: Glass fiber posts showed better fracture resistance than Quartz fiber posts. 4-mm coronal length showed more fracture resistance than 6 mm

Response of wheat (Triticum aestivum) landraces from north-western Himalaya to rice weevil (Sitophilus oryzae)

Wheat (Triticum aestivum L.) landraces (one hundred) from the north-western Himalayan region were evaluated for their response to rice weevil, Sitophilus oryzae L. under no-choice artificial infestation conditions in the laboratory. The differential reaction was based on the parameters, viz. number of adults emerged, development period, growth index (GI) and weight loss in seeds. On the basis of GI, these landraces were categorised as resistant (six landraces with GI from 0.33-0.49), moderately resistant (13 landraces, GI from 0.52-0.97), moderately susceptible (41 landraces, GI ranging from 1.01-1.50), and susceptible (41 with GI from 1.51-1.86). On the basis of seed weight loss, eight landraces were found resistant (8.3-18.5%), 32 as moderately resistant (20.1-39.5), 51 as moderately susceptible (40.3-59.9), and 10 landraces (61.4-68.8) were found susceptible. Four landraces, viz. IC393109, IC392578, IC444217 and IC589276 were found resistant both on the basis of GI and seed weight loss. These landraces can be used in breeding programme for development of wheat cultivars resistant to S. oryzae

Mycobacterium tuberculosis infection drives a type I IFN signature in lung lymphocytes

Mycobacterium tuberculosis (Mtb) infects 25% of the world\u27s population and causes tuberculosis (TB), which is a leading cause of death globally. A clear understanding of the dynamics of immune response at the cellular level is crucial to design better strategies to control TB. We use the single-cell RNA sequencing approach on lung lymphocytes derived from healthy and Mtb-infected mice. Our results show the enrichment of the type I IFN signature among the lymphoid cell clusters, as well as heat shock responses in natural killer (NK) cells from Mtb-infected mice lungs. We identify Ly6A as a lymphoid cell activation marker and validate its upregulation in activated lymphoid cells following infection. The cross-analysis of the type I IFN signature in human TB-infected peripheral blood samples further validates our results. These findings contribute toward understanding and characterizing the transcriptional parameters at a single-cell depth in a highly relevant and reproducible mouse model of TB

Source of Previous Treatment for Re-Treatment TB Cases Registered under the National TB Control Programme, India, 2010

BACKGROUND: In 2009, nearly half (289,756) of global re-treatment TB notifications are from India; no nationally-representative data on the source of previous treatment was available to inform strategies for improvement of initial TB treatment outcome. OBJECTIVES: To assess the source of previous treatment for re-treatment TB patients registered under India's Revised National TB control Programme (RNTCP). METHODOLOGY: A nationally-representative cross sectional study was conducted in a sample of 36 randomly-selected districts. All consecutively registered retreatment TB patients during a defined 15-day period in these 36 districts were contacted and the information on the source of previous treatment sought. RESULTS: Data was collected from all 1712 retreatment TB patients registered in the identified districts during the study period. The data includes information on 595 'relapse' cases, 105 'failure' cases, 437 'treatment after default (TAD)' cases and 575 're-treatment others' cases. The source of most recent previous anti-tuberculosis therapy for 754 [44% (95% CI, 38.2%-49.9%)] of the re-treatment TB patients was from providers outside the TB control programme. A higher proportion of patients registered as TAD (64%) and 'retreatment others' (59%) were likely to be treated outside the National Programme, when compared to the proportion among 'relapse' (22%) or 'failure' (6%). Extrapolated to national registration, of the 292,972 re-treatment registrations in 2010, 128,907 patients would have been most recently treated outside the national programme. CONCLUSIONS: Nearly half of the re-treatment cases registered with the national programme were most recently treated outside the programme setting. Enhanced efforts towards extending treatment support and supervision to patients treated by private sector treatment providers are urgently required to improve the quality of treatment and reduce the numbers of patients with recurrent disease. In addition, reasons for the large number of recurrent TB cases from those already treated by the national programme require urgent detailed investigation

Study of acoustic parameters of proline in lecithin-ethanol mixture at varying temperature

531-541The solution behaviour of proline in lecithin-ethanol mixture as a function of temperature and composition have been investigated by measuring velocity of sound in conjunction with density at different temperature in the range 25-40°C at an interval of 5<span style="font-family: Symbol;mso-ascii-font-family:" times="" new="" roman";mso-hansi-font-family:"times="" roman";="" mso-char-type:symbol;mso-symbol-font-family:symbol"="" lang="EN-GB">°C. Various acoustical parameters such as intermolecular free length (Lf), relative association (RA), specific acoustic impedance (z), relaxation time (<span style="font-family:Symbol; mso-bidi-font-family:" times="" new="" roman""="" lang="EN-GB">t), sound velocity number (U), molar volume (Vm) and internal pressure (pi) have been evaluated from velocity of sound, density and viscosity measurements. The intermolecular free length (Lf) varies linearly with the concentration of proline and increases with rise in temperature. The linear trend in Lf values with the concentration of proline has been supported by the trend obtained from <span style="font-family:Symbol; mso-bidi-font-family:" times="" new="" roman""="" lang="EN-GB">t values. The excess values of these parameters have also been calculated and their trends of variation are discussed in terms of the presence of significant molecular interactions between the component molecules in the mixtures. </span

Cyanuric Chloride Catalyzed Mild Protocol for Synthesis of Biologically Active Dihydro/Spiro Quinazolinones and Quinazolinone-glycoconjugates

We have developed an efficient cyanuric chloride (2,4,6-trichloro-1,3,5-triazine, TCT) catalyzed approach for the synthesis of 2,3-dihydroquinazolin-4­(1<i>H</i>)-one (<b>3a</b>–<b>3x</b>), 2-spiroquinazolinone (<b>5</b>, <b>7</b>), and glycoconjugates of 2,3-dihydroquinazolin-4­(1<i>H</i>)-one (<b>10a</b>, <b>10b</b>) derivatives. The reaction allows rapid cyclization (8–20 min) with 10 mol % cyanuric chloride to give skeletal complexity in good to excellent yield. We believe that this novel procedure may open the door for the easy generation of new and bioactive quinazolinones

Volumetric, Compressibility and Viscometric Approach to Study the Interactional Behaviour of Sodium Cholate and Sodium Deoxycholate in Aqueous Glycyl Glycine

Viscosity, speed of sound (u), and density (&rho;) have been measured in aqueous glycyl glycine solution over a temperature range from 293.15 to 313.15 K with a 5 K interlude to evaluate the volumetric and compressibility properties of bio-surfactants, namely sodium cholate (NaC; 1&ndash;20 mmol&#8729;kg&minus;1) and sodium deoxycholate (NaDC; 1&ndash;10 mmol&#8729;kg&minus;1). Density and viscosity findings provide information on both solute&ndash;solute and solute&ndash;solvent types of interactions. Many other metrics, such as apparent molar adiabatic compression (&kappa;S,&phi;), isentropic compressibility (&kappa;S), and apparent molar volume (V&phi;), have been calculated from speed of sound and density measurements, utilising experimental data. The results show that the zwitterionic end group in the glycyl glycine strongly interacts with NaDC and NaC, promoting its micellization. Since the addition of glycyl glycine causes the bio-surfactant molecules to lose their hydrophobic hydration, the observed concentration-dependent changes in apparent molar volume and apparent molar adiabatic compression are likely attributable to changes in water&ndash;water interactions. Viscous relaxation time (&tau;) increases significantly with a rise in bio-surfactant concentration and decreases with increasing temperature, which may be because of structural relaxation processes resulting from molecular rearrangement. All of the estimated parameters have been analysed for their trends with regard to the different patterns of intermolecular interaction present in an aqueous glycyl glycine solution and bio-surfactant system

Screening of water-efficient rice genotypes for dry direct seeding in South Asia

<p>Rice genotypes having high crop water productivity in dry direct-seeded rice (DDSR) need to be assessed while promoting labor- and water-saving technology. The objective of this study was to evaluate the ability of several selection indices to identify genotypes with high crop water productivity in DDSR. A set of 16 genotypes [13 recombinant inbred lines (RILs) and three genotypes as check for control] were evaluated under DDSR for two consecutive years (2013 and 2014) using two irrigation regimes [-10 kPa (non-stress) and -20 kPa (stress)]. Grain yield varied from 5.3 to 8.4 t ha^-1 and 5.9 to 8.7 t ha^-1 for irrigation regimes of -20 and -10 kPa, respectively. Yield reductions caused by water stress ranged from 2.9 to 25.1%; being lowest in RIL1649 and highest in hybrid SVH-127. The genotypes with high stress-tolerance index (STI), mean productivity (MP), geometric mean productivity (GMP) and harmonic mean productivity (HAR) were identified as the most productive genotypes for high crop water productivity in DDSR. The results implied that selection based on stress tolerance indices likes STI, MP, GMP and HAR was useful in identifying genotypes with high crop water productivity under DDSR and RIL 1649 and SVH-127 were identified as superior genotypes in this regard.</p

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Not AvailableA total eight polymorphic microsatellite loci were obtained from genomic library of Indian feather back, Chitala chitala (order Osteoglossiformes, family Notopteridae) and the 46 samples were analysed to determine genetic variation. The mean number of alleles per locus ranged from 4.50 to 5.25, and expected heterozygosity ranged from 0.124 to 0.852. Deviation from Hardy–Weinberg equilibrium expectations (P < 0.002) was observed at loci Cch2, Cch9 (Bhaghirathi) and Cch9 (Brahmaputra). The identified microsatellite loci were found promising for population genetics studies of C. chitala and related species Notopterus notopterus (family Notopteridae).ICAR-APCess (F. no. 4(44)/2002-ARS-I), New Delhi

A protective role for type I interferon signaling following infection with <i>Mycobacterium tuberculosis</i> carrying the rifampicin drug resistance-conferring RpoB mutation H445Y

Interleukin-1 (IL-1) signaling is essential for controlling virulent Mycobacterium tuberculosis (Mtb) infection since antagonism of this pathway leads to exacerbated pathology and increased susceptibility. In contrast, the triggering of type I interferon (IFN) signaling is associated with the progression of tuberculosis (TB) disease and linked with negative regulation of IL-1 signaling. However, mice lacking IL-1 signaling can control Mtb infection if infected with an Mtb strain carrying the rifampin-resistance conferring mutation H445Y in its RNA polymerase β subunit (rpoB-H445Y Mtb). The mechanisms that govern protection in the absence of IL-1 signaling during rpoB-H445Y Mtb infection are unknown. In this study, we show that in the absence of IL-1 signaling, type I IFN signaling controls rpoB-H445Y Mtb replication, lung pathology, and excessive myeloid cell infiltration. Additionally, type I IFN is produced predominantly by monocytes and recruited macrophages and acts on LysM-expressing cells to drive protection through nitric oxide (NO) production to restrict intracellular rpoB-H445Y Mtb. These findings reveal an unexpected protective role for type I IFN signaling in compensating for deficiencies in IL-1 pathways during rpoB-H445Y Mtb infection