Long -term feeding with high plant protein based diets in gilthead seabream (Sparus aurata, L.) leads to changes in the inflammatory and immune related gene expression at intestinal level

[EN] Background: In order to ensure sustainability of aquaculture production of carnivourous fish species such as the gilthead seabream (Sparus aurata, L.), the impact of the inclusion of alternative protein sources to fishmeal, including plants, has been assessed. With the aim of evaluating long-term effects of vegetable diets on growth and intestinal status of the on-growing gilthead seabream (initial weight = 129 g), three experimental diets were tested: a strict plant protein-based diet (VM), a fishmeal based diet (FM) and a plant protein-based diet with 15% of marine ingredients (squid and krill meal) alternative to fishmeal (VM+). Intestines were sampled after 154 days. Besides studying growth parameters and survival, the gene expression related to inflammatory response, immune system, epithelia integrity and digestive process was analysed in the foregut and hindgut sections, as well as different histological parameters in the foregut. Results: There were no differences in growth performance (p = 0.2703) and feed utilization (p = 0.1536), although a greater fish mortality was recorded in the VM group (p = 0.0141). In addition, this group reported a lower expression in genes related to pro-inflammatory response, as Interleukine-1 beta (il1 beta, p = 0.0415), Interleukine-6 (il6, p = 0.0347) and cyclooxigenase-2 (cox2, p = 0.0014), immune-related genes as immunoglobulin M (igm, p = 0.0002) or bacterial defence genes as alkaline phosphatase (alp, p = 0.0069). In contrast, the VM+ group yielded similar survival rate to FM (p = 0.0141) and the gene expression patterns indicated a greater induction of the inflammatory and immune markers (il1 beta, cox2 and igm). However, major histological changes in gut were not detected. Conclusions: Using plants as the unique source of protein on a long term basis, replacing fishmeal in aqua feeds for gilthead seabream, may have been the reason of a decrease in the level of different pro-inflammatory mediators (il1 beta, il6 and cox2) and immune-related molecules (igm and alp), which reflects a possible lack of local immune response at the intestinal mucosa, explaining the higher mortality observed. Krill and squid meal inclusion in vegetable diets, even at low concentrations, provided an improvement in nutrition and survival parameters compared to strictly plant protein based diets as VM, maybe explained by the maintenance of an effective immune response throughout the assay.The research has been partially funded by Vicerrectorat d'Investigacio, Innovacio i Transferencia of the Universitat Politecnica de Valencia, which belongs to the project Aquaculture feed without fishmeal (SP20120603). 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The flexibility of corporate payouts vis-à-vis capital investment: some UK evidence

Purpose: This study investigates the relative flexibility of payouts vis-à-vis investment in the UK, motivated by concerns regarding this market's distinct payout characteristics and limited relevant research. It addresses the information gap related to the use of conditional mean estimations and examines firm behavior across the investment distribution. Design/methodology/approach: The sample is an unbalanced panel of 6,173 firm-year observations, from 271 non-financial firms in the FTSE-All Share Index, during 1990–2019. Estimation methods include pooled- ordinary least squares (OLS) and firm fixed-effects regressions as well as unconditional quantile regressions with firm fixed effects. Findings: For the “average” firm results show a negative relationship between share repurchases and investment, amplified in the presence of financial constraints and growth opportunities. Quantile regressions analysis reveals heterogeneous firm behavior as this relationship becomes stronger in successive quantiles of the investment distribution and disappears at the upper/lower extremes. Results suggest that UK firms exploit the inherent flexibility of share repurchases to facilitate investment. However, this flexibility appears irrelevant to firms with extremely high/low investment, characterized by significant differences in growth opportunities, cash flows and external financing cost. Dividends and investment are independent across the investment distribution, underlining the rigidity of dividends in the UK. Originality/value: To the best of our knowledge, this is the first study to investigate the relative flexibility of payouts vis-à-vis investment in the UK, using firm-level financial data and at points other than the conditional mean. Its value lies in that it shows that share repurchases facilitate rather than impede investment and thus do not corroborate relevant concerns by economists and policymakers. Additionally, by utilizing a relatively new methodology it uncovered heterogeneous firm behavior across the investment distribution suggesting that conditional mean estimations should be applied with caution at least for highly heterogeneous samples. © 2021, Emerald Publishing Limited

Strain rate sensitivity of nanocrystalline Au films at room temperature

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Strength and Fracture Resistance of Amorphous Diamond-Like Carbon Films for MEMS

The mechanical strength and mixed mode I/II fracture toughness of hydrogen-free tetrahedral amorphous diamond-like carbon (ta-C) films, grown by pulsed laser deposition, are discussed in connection to material flaws and its microstructure. The failure properties of ta-C were obtained from films with thicknesses 0.5–3 μm and specimen widths 10–20 μm. The smallest test samples with 10 μm gage section averaged a strength of 7.3 ± 1.2 GPa, while the strength of 20-μm specimens with thicknesses 0.5–3 μm varied between 2.2–5.7 GPa. The scaling of the mechanical strength with specimen thickness and dimensions was owed to deposition-induced surface flaws, and, only in the smallest specimens, RIE patterning generated specimen sidewall flaws. The mode I fracture toughness of ta-C films is KIc=4.4±0.4 MPam, while the results from mixed mode I/II fracture experiments with cracks arbitrarily oriented in the plane of the film compared very well with theoretical predictions