A many-analysts approach to the relation between religiosity and well-being

The relation between religiosity and well-being is one of the most researched topics in the psychology of religion, yet the directionality and robustness of the effect remains debated. Here, we adopted a many-analysts approach to assess the robustness of this relation based on a new cross-cultural dataset (N=10,535 participants from 24 countries). We recruited 120 analysis teams to investigate (1) whether religious people self-report higher well-being, and (2) whether the relation between religiosity and self-reported well-being depends on perceived cultural norms of religion (i.e., whether it is considered normal and desirable to be religious in a given country). In a two-stage procedure, the teams first created an analysis plan and then executed their planned analysis on the data. For the first research question, all but 3 teams reported positive effect sizes with credible/confidence intervals excluding zero (median reported β=0.120). For the second research question, this was the case for 65% of the teams (median reported β=0.039). While most teams applied (multilevel) linear regression models, there was considerable variability in the choice of items used to construct the independent variables, the dependent variable, and the included covariates

Marine volcanosedimentary basins hosting porphyry Au-Cu deposits, Cadia Valley, New South Wales, Australia

The volcanosedimentary rocks that host the Au-rich porphyry Cu deposits of the Cadia Valley preserve the products of episodic volcanism that erupted into a large sedimentary basin. Volcanogenic sedimentation, including the Forest Reefs Volcanics, overwhelmed the fine-grained sedimentary component that characterized much of the Weemalla Formation. The Forest Reefs Volcanics evolved as a relatively low relief, multiple-vent submarine volcanic complex. The vents comprised mafic to intermediate lava flows, cryptodomes, and subvolcanic intrusions (dikes and sills). Stacked lava sequences, including hyaloclastites, massive lavas, and their reworked equivalents, are up to 1 km thick, forming significant intrabasinal topography. Explosive volcanism occurred during the late stages of Forest Reefs Volcanics deposition. These air-fall deposits, combined with coexisting shallow-water faunal assemblages, imply that volcanism became locally emergent. Continuity of sedimentation between underlying deep marine basin deposits of the Weemalla Formation and Forest Reefs Volcanics, coupled with the predominance of sheet-like, laterally continuous debris flow and other coarse-grained sedimentary deposits, implies that volcanism and related sedimentation persisted in an active sedimentary basin marginal to an oceanic island arc. Deposition of the Forest Reefs Volcanics spanned the Late Ordovician to Early Silurian. Monzonite fragments (identical to the ore-related intrusions) are abundant in sedimentary breccias found at the top of the preserved volcanic stratigraphy. This finding, combined with available absolute ages of crosscutting intrusions and associated hydrothermal alteration and mineralization, suggests that some volcanosedimentary units were deposited synchronously with or immediately after the last known porphyry-related hydrothermal event in the Cadia Valley. The Au-rich porphyry deposits were therefore emplaced into an evolving sedimentary basin with episodic intrabasinal magmatism. Permeable horizons and volcanic lithofacies can preferentially host alteration and mineralization that can extend over several kilometers in lateral extent. This finding suggests that hydrological models of fluid flow in porphyry systems need to take basin architecture into account

A full-coverage, high-resolution human chromosome 22 genomic microarray for clinical and research applications.

We have constructed the first comprehensive microarray representing a human chromosome for analysis of DNA copy number variation. This chromosome 22 array covers 34.7 Mb, representing 1.1% of the genome, with an average resolution of 75 kb. To demonstrate the utility of the array, we have applied it to profile acral melanoma, dermatofibrosarcoma, DiGeorge syndrome and neurofibromatosis 2. We accurately diagnosed homozygous/heterozygous deletions, amplifications/gains, IGLV/IGLC locus instability, and breakpoints of an imbalanced translocation. We further identified the 14-3-3 eta isoform as a candidate tumor suppressor in glioblastoma. Two significant methodological advances in array construction were also developed and validated. These include a strictly sequence defined, repeat-free, and non-redundant strategy for array preparation. This approach allows an increase in array resolution and analysis of any locus; disregarding common repeats, genomic clone availability and sequence redundancy. In addition, we report that the application of phi29 DNA polymerase is advantageous in microarray preparation. A broad spectrum of issues in medical research and diagnostics can be approached using the array. This well annotated and gene-rich autosome contains numerous uncharacterized disease genes. It is therefore crucial to associate these genes to specific 22q-related conditions and this array will be instrumental towards this goal. Furthermore, comprehensive epigenetic profiling of 22q-located genes and high-resolution analysis of replication timing across the entire chromosome can be studied using our array

The evolutionary dynamics of influenza A virus adaptation to mammalian hosts

Few questions on infectious disease are more important than understanding how and why avian influenza A viruses successfully emerge in mammalian populations, yet little is known about the rate and nature of the virus’ genetic adaptation in new hosts. Here, we measure, for the first time, the genomic rate of adaptive evolution of swine influenza viruses (SwIV) that originated in birds. By using a curated dataset of more than 24 000 human and swine influenza gene sequences, including 41 newly characterized genomes, we reconstructed the adaptive dynamics of three major SwIV lineages (Eurasian, EA; classical swine, CS; triple reassortant, TR). We found that, following the transfer of the EA lineage from birds to swine in the late 1970s, EA virus genes have undergone substantially faster adaptive evolution than those of the CS lineage, which had circulated among swine for decades. Further, the adaptation rates of the EA lineage antigenic haemagglutinin and neuraminidase genes were unexpectedly high and similar to those observed in human influenza A. We show that the successful establishment of avian influenza viruses in swine is associated with raised adaptive evolution across the entire genome for many years after zoonosis, reflecting the contribution of multiple mutations to the coordinated optimization of viral fitness in a new environment. This dynamics is replicated independently in the polymerase genes of the TR lineage, which established in swine following separate transmission from non-swine hosts